| Title : Disodium cromoglycate suppresses the induction of cysteinyl leukotriene synthesis during granulocytic differentiation in HL-60 cells - Zaitsu_2004_Respir.Med_98_235 |
| Author(s) : Zaitsu M , Honjo K , Ishii E , Hamasaki Y |
| Ref : Respir Med , 98 :235 , 2004 |
| Abstract : OBJECTIVES Disodium cromoglycate (DSCG) and nedocromil sodium are anti-asthma drugs that have a variety of physiological and biological effects. We examined whether DSCG affects the induction of cysteinyl Leukotriene (cysLT) synthesis during dimethyl sulfoxide (DMSO)-induced granulocytic differentiation in HL-60 cells.
METHODS:
HL-60 cells were differentiated to mature granulocyte-like cells by DMSO in the presence or absence of DSCG for 5 days. Then, we measured A23187-stimulated production of LTC4, an initial product of cysLTs. We also examined the mRNA expression and enzyme activity of LTC4 synthase and other LT-synthetic enzymes.
RESULTS:
The amount of LTC4 production was 732.0+/-19.0 pg/10(6) cells in DMSO-differentiated HL-60 cells. The value was significantly decreased to 420.7+/-22.7 pg/10(6) cells in the presence of DSCG at 100 microg/ml. The DMSO-induced mRNA expression and enzyme activity of LTC4 synthase was also suppressed by DSCG.
CONCLUSIONS: Our results indicate that DSCG suppresses the DMSO-induced LTC4 synthase-activity by inhibiting mRNA expression of LTC4 synthase, which might be a novel anti-allergic action of DSCG. |
| ESTHER : Zaitsu_2004_Respir.Med_98_235 |
| PubMedSearch : Zaitsu_2004_Respir.Med_98_235 |
| PubMedID: 15002759 |
Zaitsu M, Honjo K, Ishii E, Hamasaki Y (2004)
Disodium cromoglycate suppresses the induction of cysteinyl leukotriene synthesis during granulocytic differentiation in HL-60 cells
Respir Med
98 :235
Zaitsu M, Honjo K, Ishii E, Hamasaki Y (2004)
Respir Med
98 :235