| Title : Carboxylesterase, a de-esterification enzyme, catalyzes the degradation of chlorimuron-ethyl in Rhodococcus erythropolis D310-1 - Zang_2019_J.Hazard.Mater__121684 |
| Author(s) : Zang H , Wang H , Miao L , Cheng Y , Zhang Y , Liu Y , Sun S , Wang Y , Li C |
| Ref : J Hazard Mater , :121684 , 2019 |
| Abstract : Microbial degradation is considered to be the most acceptable method for degradation of chlorimuron-ethyl, a typical long-term residual sulfonylurea herbicide, but the underlying mechanism at the genetic and biochemical levels is unclear. In this work, the genome sequence of the chlorimuron-ethyl-degrading bacterium Rhodococcus erythropolis D310-1 was completed, and the gene clusters responsible for the degradation of chlorimuron-ethyl in D310-1 were predicted. A carboxylesterase gene, carE, suggested to be responsible for carboxylesterase de-esterification, was cloned from D310-1. CarE was expressed in Escherichia coli BL21 and purified to homogeneity. The active site of the chlorimuron-ethyl-degrading enzyme CarE and the biochemical activities of CarE were elucidated. The results demonstrated that CarE is involved in catalyzing the de-esterification of chlorimuron-ethyl. A carE deletion mutant strain, D310-1DeltacarE, was constructed, and the chlorimuron-ethyl degradation rate in the presence of 100mgL(-1) chlorimuron-ethyl within 120h decreased from 86.5 % (wild-type strain D310-1) to 58.2 % (mutant strain D310-1DeltacarE). Introduction of the plasmid pNit-carE restored the ability of the mutant strain to utilize chlorimuron-ethyl. This study is the first to demonstrate that carboxylesterase can catalyze the de-esterification reaction of chlorimuron-ethyl and provides new insights into the mechanism underlying the degradation of sulfonylurea herbicides and a theoretical basis for the utilization of enzyme resources. |
| ESTHER : Zang_2019_J.Hazard.Mater__121684 |
| PubMedSearch : Zang_2019_J.Hazard.Mater__121684 |
| PubMedID: 31784128 |
| Gene_locus related to this paper: rhoe4-c1a280 |
| Gene_locus related to this paper: rhoe4-c1a280 |
Zang H, Wang H, Miao L, Cheng Y, Zhang Y, Liu Y, Sun S, Wang Y, Li C (2019)
Carboxylesterase, a de-esterification enzyme, catalyzes the degradation of chlorimuron-ethyl in Rhodococcus erythropolis D310-1
J Hazard Mater
:121684
Zang H, Wang H, Miao L, Cheng Y, Zhang Y, Liu Y, Sun S, Wang Y, Li C (2019)
J Hazard Mater
:121684