Zhang_1992_Immunology_77_571

Twenty-six monoclonal antibodies of five different isotypes and reactive against distinct parts (alpha, beta, gamma, delta-subunits) of the nicotinic acetylcholine receptor (AChR) from Torpedo californica were screened for their capacity to enhance activation of AChR-specific CD4+ autoreactive T cells. The T-cell line (LR) used in this study recognized an epitope (98-116) on the alpha-subunit of Torpedo AChR. Four monoclonal antibodies bearing a gamma 2b isotype and recognizing an epitope on the Torpedo AChR alpha-subunit, especially the main immunogenic region (MIR), were able to enhance T-cell activation in a dose-response manner. Four further gamma 2b isotype monoclonal antibodies, recognizing epitopes other than the AChR alpha-subunit, had no effect. Monoclonal antibodies of other isotypes (IgM, IgG1, IgG2a, IgG2c), irrespective of their subunit specificity, were unable to influence the T-cell response. Thus, the enhancement requires a IgG2b isotype, and both the antibody and the T-cell recognize an epitope on the same subunit. We have previously shown that AChR-specific B cells are directly able to present antigen to AChR-specific T-cell lines in a privileged way. The present data demonstrate that B cells are also capable of enhancing indirectly the immunogenicity of autoantigens via their humoral antibodies.