Zhang_2002_Neurosci.Lett_319_107

Reference

Title : Spermidine antagonizes the inhibitory effect of huperzine A on [3H]dizocilpine (MK-801) binding in synaptic membrane of rat cerebral cortex - Zhang_2002_Neurosci.Lett_319_107
Author(s) : Zhang YH , Zhao XY , Chen XQ , Wang Y , Yang HH , Hu GY
Ref : Neuroscience Letters , 319 :107 , 2002
Abstract :

Huperzine A, a novel cholinesterase inhibitor, was found to inhibit the N-methyl-D-aspartate (NMDA) receptors in the brain. In this study, the mechanisms of the NMDA receptor inhibition were investigated using [3H]dizocilpine (MK-801) binding in synaptic membrane of rat cerebral cortex. Changing the concentrations of L-glutamate and L-glycine did not alter the potency of huperzine A. Spermidine caused rightward shift of the concentration-response curve of huperzine A, and considerably increased its IC(50) value. Huperzine A did not affect the potency of unlabeled (+)-MK-801 in [3H]MK-801 binding. Saturation binding studies reveal that huperzine A exerts a negative allosteric modulation on the MK-801 binding site within the NMDA receptor-channel. The results suggest that huperzine A is a non-competitive antagonist of the NMDA receptors, acting at one of the polyamine binding sites.

PubMedSearch : Zhang_2002_Neurosci.Lett_319_107
PubMedID: 11825682

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Citations formats

Zhang YH, Zhao XY, Chen XQ, Wang Y, Yang HH, Hu GY (2002)
Spermidine antagonizes the inhibitory effect of huperzine A on [3H]dizocilpine (MK-801) binding in synaptic membrane of rat cerebral cortex
Neuroscience Letters 319 :107

Zhang YH, Zhao XY, Chen XQ, Wang Y, Yang HH, Hu GY (2002)
Neuroscience Letters 319 :107