Title : Calmodulin interaction with peptides from G-protein coupled receptors measured with S-Tag labeling - Zhang_2005_Biochem.Biophys.Res.Commun_333_390 |
Author(s) : Zhang Y , Wang D , Sadee W |
Ref : Biochemical & Biophysical Research Communications , 333 :390 , 2005 |
Abstract :
We have developed a quantitative assay of calmodulin (CaM) binding to S-Tag labeled peptides derived from G-protein coupled receptor (GPCR) sequences. CaM binding of peptides derived from the third intracellular loop (i3) of mu opioid receptor (MOR) was confirmed and the CaM-binding motif refined. A MORi3 peptide with a Lys > Ala substitution--shown to reduce CaM-binding of intact MOR--bound fivefold less avidly than the wild-type peptide. Screening peptides derived from i3 loops of other GPCR families confirmed 5HT1A, and identified muscarinic receptor 3, and melanocortin receptor 1, as proteins carrying CaM-binding domains. The use of S-Tag labeling can serve for rapid screening of putative CaM-binding domains in GPCRs. |
PubMedSearch : Zhang_2005_Biochem.Biophys.Res.Commun_333_390 |
PubMedID: 15950946 |
Zhang Y, Wang D, Sadee W (2005)
Calmodulin interaction with peptides from G-protein coupled receptors measured with S-Tag labeling
Biochemical & Biophysical Research Communications
333 :390
Zhang Y, Wang D, Sadee W (2005)
Biochemical & Biophysical Research Communications
333 :390