Zhang_2006_Nan.Fang.Yi.Ke.Da.Xue.Xue.Bao_26_1053

OBJECTIVE To screen the peptide inhibitor of acetylcholinesterase (AChE) from 12-mer random phage display peptide library. METHODS: Human AChE was used as the target to screen its binding peptides from 12-mer random phage display peptide library. The positive phage clones were isolated after three rounds of biopanning followed then by sequence analysis and their activity evaluation. RESULTS: Six positive phage clones binding to human AChE were obtained, and 4 of them sharing the conservative sequence W(S/P)HY inhibited the enzyme activity of AChE. CONCLUSION: Acquisition of AChE inhibitor from phage display library provides clues for designing peptide inhibitors of AChE.