Zhang_2021_Appl.Biochem.Biotechnol__

Reference

Title : Identification and Characterization of a Novel Carboxylesterase Belonging to Family VIII with Promiscuous Acyltransferase Activity Toward Cyanidin-3-O-Glucoside from a Soil Metagenomic Library - Zhang_2021_Appl.Biochem.Biotechnol__
Author(s) : Zhang Y , Ding L , Yan Z , Zhou D , Jiang J , Qiu J , Xin Z
Ref : Appl Biochem Biotechnol , : , 2021
Abstract :

An alkaline esterase, designated as EstXT1, was identified through functional screening from a metagenomic library. Sequence analysis revealed that EstXT1 belonged to the family VIII carboxylesterases and contained a characteristic conserved S-x-x-K motif and a deduced catalytic triad Ser56-Lys59-Tyr165. EstXT1 exhibited the strongest activity toward methyl ferulate at pH 8.0 and temperature 55 degreesC and retained over 80% of its original activity after incubation in the pH range of 7.0-10.6 buffers. Biochemical characterization of the recombinant enzyme showed that it was activated by Zn(2+) and Co(2+) metal ion, while inhibited by Cu(2+) and CTAB. EstXT1 exhibited significant promiscuous acyltransferase activity preferred to the acylation of benzyl alcohol acceptor using short-chain pNP-esters (C2-C8) as acyl-donors. A structure-function analysis indicated that a WAG motif is essential to acyltransferase activity. This is the first report example that WAG motif plays a pivotal role in acyltransferase activity in family VIII carboxylesterases beside WGG motif. Further experiment indicated that EstXT1 successfully acylated cyanidin-3-O-glucoside in aqueous solution. The results from the current investigation provided new insights for the family VIII carboxylesterase and lay a foundation for the potential applications of EstXT1 in food and biotechnology fields.

PubMedSearch : Zhang_2021_Appl.Biochem.Biotechnol__
PubMedID: 34255285

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Citations formats

Zhang Y, Ding L, Yan Z, Zhou D, Jiang J, Qiu J, Xin Z (2021)
Identification and Characterization of a Novel Carboxylesterase Belonging to Family VIII with Promiscuous Acyltransferase Activity Toward Cyanidin-3-O-Glucoside from a Soil Metagenomic Library
Appl Biochem Biotechnol :

Zhang Y, Ding L, Yan Z, Zhou D, Jiang J, Qiu J, Xin Z (2021)
Appl Biochem Biotechnol :