Zhang_2024_Neurochem.Res__

Reference

Title : Sulphated Fucooligosaccharide from Sargassum Horneri: Structural Analysis and Anti-Alzheimer Activity - Zhang_2024_Neurochem.Res__
Author(s) : Zhang LJ , Zhang HZ , Liu YW , Tang M , Jiang YJ , Li FN , Guan LP , Jin QH
Ref : Neurochem Res , : , 2024
Abstract :

In the present study, sulfated polysaccharides were obtained by digestion of Sargassum horneri and preparation with enzyme-assisted extraction using three food-grade enzymes, and their anti- Alzheimer's activities were investigated. The results demonstrated that the crude sulfated polysaccharides extracted using AMGSP, CSP and VSP dose-dependently (25-100 microg.mL(- 1)) raised the spontaneous alternating manner (%) in the Y maze experiment of mice and reduced the escape latency time in Morris maze test. AMGSP, CSP and VSP also exhibited good anti-AChE and moderate anti-BuChE activities. CSP displayed the best inhibitory efficacy against AChE. with IC(50) values of 9.77 microM. And, CSP also exhibited good inhibitory selectivity of AChE over BuChE. Next, CSP of the best active crude extract was separated by the preparation type high performance liquid phase to obtain the sulphated fucooligosaccharide section: SFcup (3-alpha-L-fucp(2-SO(3)(-))-14-alpha-L-fucp(2,3-SO(3)(-))-1section), SFcup showed a best inhibitory efficacy against AChE with IC(50) values of 4.03 microM. The kinetic research showed that SFcup inhibited AChE through dual binding sites. Moreover, the molecular docking of SFcup at the AChE active site was in accordance with the acquired pharmacological results.

PubMedSearch : Zhang_2024_Neurochem.Res__
PubMedID: 38305960

Related information

Citations formats

Zhang LJ, Zhang HZ, Liu YW, Tang M, Jiang YJ, Li FN, Guan LP, Jin QH (2024)
Sulphated Fucooligosaccharide from Sargassum Horneri: Structural Analysis and Anti-Alzheimer Activity
Neurochem Res :

Zhang LJ, Zhang HZ, Liu YW, Tang M, Jiang YJ, Li FN, Guan LP, Jin QH (2024)
Neurochem Res :