Zhang_2025_J.Hazard.Mater_494_138441

The presence of pesticides, antibiotics, and microplastics in aquatic environments poses a significant threat because of their persistence and potential harm to aquatic life and human health. However, few studies have explored their combined effects on bioaccumulation and toxicity in edible bivalves. This study examined the bioaccumulation and toxicological impacts of thifluzamide (TF) and enrofloxacin (ENR) on oxidative stress, neurotoxicity, detoxification, and metabolism in Mytilus coruscus after 4 weeks of exposure at the environmental level. The findings indicated that coexposure to TF and ENR or the presence of microplastic polystyrene (PS) increased TF and ENR accumulation in mussels and caused oxidative damage, as evidenced by elevated catalase and glutathione transferase activities and increased malondialdehyde (MDA) levels. Notably, compared with single exposures, coexposure to PS+TF, PS+ENR, or TF+ENR generally increased the MDA content, reduced acetylcholinesterase activity, and increased detoxification gene expression. Metabolomic analysis revealed that TF, ENR, and PS, either alone or combined, significantly disrupted multiple metabolic pathways by altering levels of glycerophospholipids, eicosanoids, amino acids, and nucleotides. Coexposure particularly worsened glycerophospholipid and arachidonic acid metabolism disturbances. These results suggest that combined exposure to TF, ENR or PS exacerbated the ecotoxicological effects of TF and ENR on M. coruscus. Taken together, the results of the present study could enhance our understanding of the environmental effects resulting from multipollutant interactions and their potential risks to seafood security.