| Title : Spatially confined multi-enzyme cascade nanoreactor for portable and real-time pesticide detection - Zhang_2026_J.Nanobiotechnology__ |
| Author(s) : Zhang H , Li Y , Bi Q , Zhu H , Wang H , Wang Z , Ma Z , Liu H , Zhu X , Cao J , Wang X , Tang Z , Chen X , Tang L , Peng K , Zhang W , Shu J , Gong C , Zhou S , Feng K , Liu D , Chen R , Li J , Zhang Z , Li S |
| Ref : J Nanobiotechnology , : , 2026 |
|
Abstract :
Multi-enzyme cascade reactions offer powerful amplification and accelerated biotransformation, yet their implementation in free-solution systems is limited by enzyme instability, diffusion losses, and low coupling efficiency. Here, we reported a spatially confined multi-enzyme cascade nanoreactor (AChE/CHO/HRP@MSN) constructed by co-immobilizing acetylcholinesterase (AChE), choline oxidase (CHO), and horseradish peroxidase (HRP) within amino-functionalized mesoporous silica nanoparticles (MSN-NH(2)). The ordered mesoporous architecture provides high loading capacity, efficient substrate channeling, and enhanced structural stability, enabling tightly coupled multi-step biocatalysis even in complex environments. The AChE/CHO/HRP@MSN platform supports dual-mode colorimetric and fluorescent sensing of organophosphorus pesticides (OPs) and enables real-time monitoring of pesticide degradation in plants. By coupling with a smartphone-readable hydrogel matrix, the system enables portable signal output and on-site visual monitoring of OP residues. This spatially organized, cascade-driven strategy not only addresses longstanding limitations in multi-enzyme system design but also provides a versatile framework for programmable biosensing in complex environments. |
| PubMedSearch : Zhang_2026_J.Nanobiotechnology__ |
| PubMedID: 42265740 || 42277796 |
Zhang H, Li Y, Bi Q, Zhu H, Wang H, Wang Z, Ma Z, Liu H, Zhu X, Cao J, Wang X, Tang Z, Chen X, Tang L, Peng K, Zhang W, Shu J, Gong C, Zhou S, Feng K, Liu D, Chen R, Li J, Zhang Z, Li S (2026)
Spatially confined multi-enzyme cascade nanoreactor for portable and real-time pesticide detection
J Nanobiotechnology
:
Zhang H, Li Y, Bi Q, Zhu H, Wang H, Wang Z, Ma Z, Liu H, Zhu X, Cao J, Wang X, Tang Z, Chen X, Tang L, Peng K, Zhang W, Shu J, Gong C, Zhou S, Feng K, Liu D, Chen R, Li J, Zhang Z, Li S (2026)
J Nanobiotechnology
: