| Title : Biodegradation pathway of di-(2-ethylhexyl) phthalate by a novel Rhodococcus pyridinivorans XB and its bioaugmentation for remediation of DEHP contaminated soil - Zhao_2018_Sci.Total.Environ_640-641_1121 |
| Author(s) : Zhao HM , Hu RW , Chen XX , Chen XB , Lu H , Li YW , Li H , Mo CH , Cai QY , Wong MH |
| Ref : Sci Total Environ , 640-641 :1121 , 2018 |
| Abstract : A novel bacterial strain designated as Rhodococcus pyridinivorans XB, capable of utilizing various endocrine disruptor phthalates or phthalic acid (PA) as sole source of carbon and energy, was isolated from activated sludge. Under the optimal culture conditions (pH7.08, 30.4 degrees C, inoculum size (OD600 nm) of 0.6) obtained by response surface methodology, di-(2-ethylhexyl) phthalate (DEHP, 200mg/L) could be degraded by strain XB with a removal rate of 98% within 48h. Under the observation of an atomic force microscope, it was confirmed that DEHP did not inhibit the growth of strain XB which might produce some extracellular polymeric substances as a response to DEHP stress, resulting in rapid degradation of DEHP. At initial concentrations of 50-800mg/L DEHP, its degradation curves were well fitted with the first-order kinetic model, and the half-life of DEHP degradation varied from 5.44 to 23.5h. The degradation intermediates of DEHP were identified by both GC-MS and high performance liquid chromatography-time of flight-mass spectrometry (HPLC-TOF-MS). Significant up-regulation was observed for the relative expression levels of genes (i.e., phthalate hydrolase, PA 3,4-dioxygenase, protocatechuate 3,4-alpha and 3,4-beta dioxygenase) involved in DEHP degradation determined by real-time quantitative PCR (RT-qPCR). A DEHP biodegradation pathway by strain XB was proposed based on the identified intermediates and the degrading genes. Bioaugmentation of DEHP-contaminated soils with strain XB could efficiently promote DEHP removal, offering great potential in bioremediation of DEHP-contaminated environment. |
| ESTHER : Zhao_2018_Sci.Total.Environ_640-641_1121 |
| PubMedSearch : Zhao_2018_Sci.Total.Environ_640-641_1121 |
| PubMedID: 30021277 |
Zhao HM, Hu RW, Chen XX, Chen XB, Lu H, Li YW, Li H, Mo CH, Cai QY, Wong MH (2018)
Biodegradation pathway of di-(2-ethylhexyl) phthalate by a novel Rhodococcus pyridinivorans XB and its bioaugmentation for remediation of DEHP contaminated soil
Sci Total Environ
640-641 :1121
Zhao HM, Hu RW, Chen XX, Chen XB, Lu H, Li YW, Li H, Mo CH, Cai QY, Wong MH (2018)
Sci Total Environ
640-641 :1121