Zhao_2021_World.J.Microbiol.Biotechnol_37_212

Reference

Title : Characterization and genomic analysis of an efficient dibutyl phthalate degrading bacterium Microbacterium sp. USTB-Y - Zhao_2021_World.J.Microbiol.Biotechnol_37_212
Author(s) : Zhao Z , Liu C , Xu Q , Ahmad S , Zhang H , Pang Y , Aikemu A , Liu Y , Yan H
Ref : World J Microbiol Biotechnol , 37 :212 , 2021
Abstract :

A promising bacterial strain for biodegrading dibutyl phthalate (DBP) was successfully isolated from activated sludge and characterized as a potential novel Microbacterium sp. USTB-Y based on 16S rRNA sequence analysis and whole genome average nucleotide identity (ANI). Initial DBP of 50 mg/L could be completely biodegraded by USTB-Y both in mineral salt medium and in DBP artificially contaminated soil within 12 h at the optimal culture conditions of pH 7.5 and 30 degC, which indicates that USTB-Y has a strong ability in DBP biodegradation. Phthalic acid (PA) was identified as the end-product of DBP biodegraded by USTB-Y using GC/MS. The draft genome of USTB-Y was sequenced by Illumina NovaSeq and 29 and 188 genes encoding for putative esterase/carboxylesterase and hydrolase/alpha/beta hydrolase were annotated based on NR (non redundant protein sequence database) analysis, respectively. Gene3781 and gene3780 from strain USTB-Y showed 100% identity with dpeH and mpeH from Microbacterium sp. PAE-1. But no phthalate catabolic gene (pht) cluster was found in the genome of strain USTB-Y. The results in the present study are valuable for obtaining a more holistic understanding on diverse genetic mechanisms of PAEs biodegrading Microbacterium sp. strains.

PubMedSearch : Zhao_2021_World.J.Microbiol.Biotechnol_37_212
PubMedID: 34738191
Gene_locus related to this paper: 9mico-DpeH , 9mico-MpeH

Related information

Inhibitor Phthalate
Substrate Phthalate    Diisobutyl-phthalate
Gene_locus Phthalate    Diisobutyl-phthalate    9mico-DpeH    9mico-MpeH

Citations formats

Zhao Z, Liu C, Xu Q, Ahmad S, Zhang H, Pang Y, Aikemu A, Liu Y, Yan H (2021)
Characterization and genomic analysis of an efficient dibutyl phthalate degrading bacterium Microbacterium sp. USTB-Y
World J Microbiol Biotechnol 37 :212

Zhao Z, Liu C, Xu Q, Ahmad S, Zhang H, Pang Y, Aikemu A, Liu Y, Yan H (2021)
World J Microbiol Biotechnol 37 :212