Zheng_2019_Protein.Expr.Purif_155_1

Pichia pastoris expression is a mature and efficient eukaryotic expression system. In this work, Aspergillus oryzae lipase (AOL, with the molecular mass of 28 kDa), which can perform highly stereoselective hydrolysis of (R, S)-methyl 2-(4-hydroxyphenoxy) propanoate, was expressed in P. pastoris X-33. The specific activity of AOL was 432 U/mg, which was obtained by fed-batch cultivation in a 5 L bioreactor using a methanol feeding strategy. After fermentation, the supernatant was concentrated by ultrafiltration with a 10 kDa cut-off membrane and purified with DEAE-Sepharose FF ion-exchange chromatography and phenyl Seflnose 6 FF hydrophobic interaction chromatography. The purified lipase activity reached 5509 U/mg. AOL showed high activity toward short-chain triacylglyceride (C(4)), and the optimum temperature and pH were 40 degreesC and 8.0, respectively. The purified enzyme activity was inhibited by Zn(2+) and Cu(2+). Moreover, the K(m) and V(max) values were 1 mM and 32.89 mmol/min, respectively.