Title : Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate - Zhong_2001_Appl.Environ.Microbiol_67_5771 |
Author(s) : Zhong Z , Toukdarian A , Helinski D , Knauf V , Sykes S , Wilkinson JE , O'Bryne C , Shea T , Deloughery C , Caspi R |
Ref : Applied Environmental Microbiology , 67 :5771 , 2001 |
Abstract :
An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15. The complete nucleotide sequence of pSD15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. The most striking feature was the occurrence of five putative agarase genes. Loss of the plasmid, which occurred at a surprisingly high frequency, was associated with loss of agarase activity, supporting the sequence analysis results. |
PubMedSearch : Zhong_2001_Appl.Environ.Microbiol_67_5771 |
PubMedID: 11722934 |
Gene_locus related to this paper: micsp-Q93PA9 |
Gene_locus | micsp-Q93PA9 |
Zhong Z, Toukdarian A, Helinski D, Knauf V, Sykes S, Wilkinson JE, O'Bryne C, Shea T, Deloughery C, Caspi R (2001)
Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate
Applied Environmental Microbiology
67 :5771
Zhong Z, Toukdarian A, Helinski D, Knauf V, Sykes S, Wilkinson JE, O'Bryne C, Shea T, Deloughery C, Caspi R (2001)
Applied Environmental Microbiology
67 :5771