Zhou_2012_Fish.Physiol.Biochem_38_849

Fish suffer from anemia and hypovolemic hypotensive shock after in vivo exposure with microcystins.However except for in vivo causes for anemia and hypotension an in vitro study of fish erythrocytes exposed to MC is necessary For a better understanding of hematology toxicity of MC the main aim of the present study was to investigate the toxic effects of microcystin on fish erythrocytes in vitro Crucian carp erythrocytes were incubated in vitro with microcystin-LR MC-LR at doses of 0 1 10 100 and 1,000 nM.The level of lipid peroxidate significantly increased in MC-LR treatment groups Glutathione decreased after exposure to MC-LR The activities of antioxidative enzymes including superoxide dismutase catalase,glutathione peroxidase and glutathione-S-transferase,were significantly increased after exposure with MC-LR.The hemolysis was significantly increased while the activities of acetylcholinesterase Na-K-ATPase and Ca2+-Mg2+-ATPase were significantly decreased In addition pathological alterations in agglomerated and jagged erythrocytes were observed in blood smears The findings indicate that damages to erythrocytes should also be responsible for anemia and hypotensive shock or even death.