| Title : [Comparative study of enzymatic activity of cholinesterases of different origin in thionaphthylacetate hydrolysis reactions] - Zhukovskii_2004_Ukr.Biokhim.Zh.(1999)_76_151 |
| Author(s) : Zhukovskii Iu G , Kuznetsova LP , Sochilina EE , Veksler KV |
| Ref : Ukr Biokhim Zh (1999) , 76 :151 , 2004 |
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Abstract :
A comparative determination of kinetic parameters V and Km in the reaction of hydrolysis thionaphthylacetate and well known substrate acetylthiocholine by choline esterases from different sources was conducted. It is shown that butyrylcholine esterases hydrolyze thionaphthylacetate with velocity comparable with that of hydrolysis of acetylthiocholine, while acetylcholine esterases and propionylcholine esterases hydrolyze this substrate several times slower than acetylthiocholine. The values of Km in the reactions of hydrolysis of thionaphthylacetate for all studied cholinesterases is an order higher than for acetylthiocholine except cholinesterase of blood serum of fish. This value for the latter enzyme is practically equal. |
| PubMedSearch : Zhukovskii_2004_Ukr.Biokhim.Zh.(1999)_76_151 |
| PubMedID: 16100913 |
Zhukovskii Iu G, Kuznetsova LP, Sochilina EE, Veksler KV (2004)
[Comparative study of enzymatic activity of cholinesterases of different origin in thionaphthylacetate hydrolysis reactions]
Ukr Biokhim Zh (1999)
76 :151
Zhukovskii Iu G, Kuznetsova LP, Sochilina EE, Veksler KV (2004)
Ukr Biokhim Zh (1999)
76 :151