Zorn_2026_Efsa.j_24_e10174

The food enzyme triacylglycerol lipase (triacylglycerol acylhydrolase EC 3.1.1.3) is produced with the non-genetically modified Burkholderia stagnalis strain PL266-QLM by Meito Sangyo Co., Ltd. In a previous evaluation, the Panel had concluded that this food enzyme posed a risk of promoting the spread of antimicrobial resistance genes. As a follow-up, the applicant submitted a new application for this enzyme intended to be used in three food manufacturing processes. Since residual amounts of food enzyme-total organic solids (TOS) are removed in these three processes, a dietary exposure was not calculated. The new data provided confirmed the presence of DNA from the production strain in the food enzyme. Based on the new and the previously evaluated data, the Panel reiterates the previous conclusion that this food enzyme poses a risk of promoting the spread of antimicrobial resistance genes in the environment, and therefore its use cannot be considered safe.