Title : Covalent immobilization of Agrobacterium radiobacter epoxide hydrolase on ethylenediamine functionalised epoxy supports for biocatalytical synthesis of (R)-epichlorohydrin - Zou_2016_Biotechnol.Lett_38_1579 |
Author(s) : Zou SP , Wang ZC , Qin C , Zheng YG |
Ref : Biotechnol Lett , 38 :1579 , 2016 |
Abstract :
OBJECTIVE: To improve the operational stability and reusability of an epoxide hydrolase (EH) for the biosynthesis of optically active epoxides. RESULTS: A covalently immobilization strategy was employed to improve the stability of Agrobacterium radiobacter EH by using ethylenediamine (EDA)-functionalised epoxy resin LX-1000EP as carrier. Under the optimal conditions, the activity recovery of immobilized enzyme was 72 % and the specific activity was 634 U/g. Immobilized EH exhibited significantly enhanced thermal stability with a half-life of more than 6.8-fold at 50 degreesC than that of the free enzyme. A gram-scale kinetic resolution of (R,S)-epichlorohydrin using immobilized preparation as biocatalyst was performed and (R)-epichlorohydrin was obtained with 35 % yield and 99 % enantiomeric excess. The immobilized EH showed good operational stability and even after six reactions, it retained >85 % of the initial activity. CONCLUSION: The operational stability and recyclability of immobilized EH on an EDA-functionalized epoxy supports demonstrated its potential for producing (R)-epichlorohydrin. |
PubMedSearch : Zou_2016_Biotechnol.Lett_38_1579 |
PubMedID: 27379653 |
Substrate | Epichlorohydrin |
Zou SP, Wang ZC, Qin C, Zheng YG (2016)
Covalent immobilization of Agrobacterium radiobacter epoxide hydrolase on ethylenediamine functionalised epoxy supports for biocatalytical synthesis of (R)-epichlorohydrin
Biotechnol Lett
38 :1579
Zou SP, Wang ZC, Qin C, Zheng YG (2016)
Biotechnol Lett
38 :1579