Zuo_2020_Insect.Sci_27_1224

Reference

Title : Genetic analysis and molecular detection of resistance to chlorpyrifos mediated by the A216S substitution in acetylcholinesterase-1 in the plant bug Apolygus lucorum - Zuo_2020_Insect.Sci_27_1224
Author(s) : Zuo KR , Yang YH , Wu YD , Wu SW
Ref : Insect Sci , 27 :1224 , 2020
Abstract :

The green plant bug Apolygus lucorum is a major pest of Bt cotton in China. Previously, we reported that chlorpyrifos resistance in a laboratory selected strain of A. lucorum (BZ-R) is associated with the homozygosis of an allele in the ace-1 gene encoding an alanine to serine substitution at position 216 of acetylcholinesterase-1. Here we describe the results of crosses between the resistant BZ-R strain (41-fold to chlorpyrifos) and the unselected susceptible BZ-S strain homozygous for the wild type alanine allele at position 216. Resistance to chlorpyrifos was inherited as a semi-dominant trait mainly controlled by a single autosomal gene and co-segregates strongly but not completely with the serine substitution in ace-1. Synergism bioassays and enzyme assays showed that minor contributions to resistance are also made by enhanced cytochrome P450 and carboxylesterase activities. A survey of 25 field populations from five Chinese provinces showed strong positive correlations between LC50 s against chlorpyrifos and S216 allele and genotype frequencies, although the most tolerant populations still only show 40%-50% S216 allele frequencies. The results above provide important information for designing effective resistance monitoring and management strategies for A. lucorum in China. This article is protected by copyright. All rights reserved.

PubMedSearch : Zuo_2020_Insect.Sci_27_1224
PubMedID: 31846210
Gene_locus related to this paper: apolu-ACHE1

Related information

Mutation A216S_apolu-ACHE1
Gene_locus apolu-ACHE1

Citations formats

Zuo KR, Yang YH, Wu YD, Wu SW (2020)
Genetic analysis and molecular detection of resistance to chlorpyrifos mediated by the A216S substitution in acetylcholinesterase-1 in the plant bug Apolygus lucorum
Insect Sci 27 :1224

Zuo KR, Yang YH, Wu YD, Wu SW (2020)
Insect Sci 27 :1224