| Title : Effects of cholinergic stimulation with pyridostigmine bromide on chronic chagasic cardiomyopathic mice - de Cuba_2014_Mediators.Inflamm_2014_475946 |
| Author(s) : de Cuba MB , Ribeiro Machado MP , Farnesi TS , Alves AC , Martins LA , de Oliveira LF , Capitelli CS , Leite CF , Vinicius Silva M , Machado JR , Kappel HB , Sales de Campos H , Paiva L , da Silva Gomes NL , Guimaraes Faleiros AC , Britto CF , Savino W , Moreira OC , Rodrigues V, Jr. , Montano N , Lages-Silva E , Ramirez LE , Dias da Silva VJ |
| Ref : Mediators Inflamm , 2014 :475946 , 2014 |
|
Abstract :
The aim of the present study was to assess the effects of an anticholinesterase agent, pyridostigmine bromide (Pyrido), on experimental chronic Chagas heart disease in mice. To this end, male C57BL/6J mice noninfected (control:Con) or chronically infected (5 months) with Trypanosoma cruzi (chagasic:Chg) were treated or not (NT) with Pyrido for one month. At the end of this period, electrocardiogram (ECG); cardiac autonomic function; heart histopathology; serum cytokines; and the presence of blood and tissue parasites by means of immunohistochemistry and PCR were assessed. In NT-Chg mice, significant changes in the electrocardiographic, autonomic, and cardiac histopathological profiles were observed confirming a chronic inflammatory response. Treatment with Pyrido in Chagasic mice caused a significant reduction of myocardial inflammatory infiltration, fibrosis, and hypertrophy, which was accompanied by a decrease in serum levels of IFNgamma with no change in IL-10 levels, suggesting a shift of immune response toward an anti-inflammatory profile. Lower nondifferent numbers of parasite DNA copies were observed in both treated and nontreated chagasic mice. In conclusion, our findings confirm the marked neuroimmunomodulatory role played by the parasympathetic autonomic nervous system in the evolution of the inflammatory-immune response to T. cruzi during experimental chronic Chagas heart disease in mice. |
| PubMedSearch : de Cuba_2014_Mediators.Inflamm_2014_475946 |
| PubMedID: 25221388 |
| Inhibitor | Pyridostigmine |
de Cuba MB, Ribeiro Machado MP, Farnesi TS, Alves AC, Martins LA, de Oliveira LF, Capitelli CS, Leite CF, Vinicius Silva M, Machado JR, Kappel HB, Sales de Campos H, Paiva L, da Silva Gomes NL, Guimaraes Faleiros AC, Britto CF, Savino W, Moreira OC, Rodrigues V, Jr., Montano N, Lages-Silva E, Ramirez LE, Dias da Silva VJ (2014)
Effects of cholinergic stimulation with pyridostigmine bromide on chronic chagasic cardiomyopathic mice
Mediators Inflamm
2014 :475946
de Cuba MB, Ribeiro Machado MP, Farnesi TS, Alves AC, Martins LA, de Oliveira LF, Capitelli CS, Leite CF, Vinicius Silva M, Machado JR, Kappel HB, Sales de Campos H, Paiva L, da Silva Gomes NL, Guimaraes Faleiros AC, Britto CF, Savino W, Moreira OC, Rodrigues V, Jr., Montano N, Lages-Silva E, Ramirez LE, Dias da Silva VJ (2014)
Mediators Inflamm
2014 :475946