| Title : A fluorimetric method for measuring lipase activity based on umbelliferyl esters - de Laborde de Monpezat_1990_Lipids_25_661 |
| Author(s) : de Laborde de Monpezat T , de Jeso B , Butour JP , Chavant L , Sancholle M |
| Ref : Lipids , 25 :661 , 1990 |
| Abstract : A new fluorimetric method for measuring lipase activity is described. The fluorescent compound umbelliferone (UMB) was esterified with various unsaturated and saturated fatty acids of different carbon chain lengths, and the non-fluorescent esters were used as substrates for measuring the activity of two lipases purified from Rhizopus delemar and Candida cylindracea. The fluorescence intensity of UMB liberated by hydrolysis of the substrates gives an indication of the lipase activity towards the respective esters. UMB was found to be somewhat more sensitive and stable than 4-methylumbelliferone (4-MU), which has been used previously. The highly sensitive method (nmoles UMB/mL/hr) described here was tested on five different esters. The method has the advantage of not requiring extraction of the lipolysis products. It also can be carried out quickly, in two to three minutes, and thus lends itself to the screening of lipase-producing micro-organisms. The method offers an interesting alternative or can be complementary to assays based on the use of 4-methylumbelliferone esters as substrates. |
| ESTHER : de Laborde de Monpezat_1990_Lipids_25_661 |
| PubMedSearch : de Laborde de Monpezat_1990_Lipids_25_661 |
| PubMedID: |
de Laborde de Monpezat T, de Jeso B, Butour JP, Chavant L, Sancholle M (1990)
A fluorimetric method for measuring lipase activity based on umbelliferyl esters
Lipids
25 :661
de Laborde de Monpezat T, de Jeso B, Butour JP, Chavant L, Sancholle M (1990)
Lipids
25 :661