| Title : Screening of food grade lipases to be used in esterification and interesterification reactions of industrial interest - de Paula_2010_Appl.Biochem.Biotechnol_160_1146 |
| Author(s) : de Paula AV , Nunes GF , Silva Jde L , de Castro HF , dos Santos JC |
| Ref : Appl Biochem Biotechnol , 160 :1146 , 2010 |
|
Abstract :
Seven food grade commercially available lipases were immobilized by covalent binding on polysiloxane-polyvinyl alcohol (POS-PVA) hybrid composite and screened to mediate reactions of industrial interest. The synthesis of butyl butyrate and the interesterification of tripalmitin with triolein were chosen as model reactions. The highest esterification activity (240.63 microM/g min) was achieved by Candida rugosa lipase, while the highest interesterification yield (31%, in 72 h) was achieved by lipase from Rhizopus oryzae, with the production of about 15 mM of the triglycerides C(50) and C(52). This lipase also showed a good performance in butyl butyrate synthesis, with an esterification activity of 171.14 microM/g min. The results demonstrated the feasibility of using lipases from C. rugosa for esterification and R. oryzae lipase for both esterification and interesterification reactions. |
| PubMedSearch : de Paula_2010_Appl.Biochem.Biotechnol_160_1146 |
| PubMedID: 19263247 |
de Paula AV, Nunes GF, Silva Jde L, de Castro HF, dos Santos JC (2010)
Screening of food grade lipases to be used in esterification and interesterification reactions of industrial interest
Appl Biochem Biotechnol
160 :1146
de Paula AV, Nunes GF, Silva Jde L, de Castro HF, dos Santos JC (2010)
Appl Biochem Biotechnol
160 :1146