| Title : L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches - van der Kaaij_2004_Appl.Environ.Microbiol_70_1869 |
| Author(s) : van der Kaaij H , Desiere F , Mollet B , Germond JE |
| Ref : Applied Environmental Microbiology , 70 :1869 , 2004 |
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Abstract :
Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase. |
| PubMedSearch : van der Kaaij_2004_Appl.Environ.Microbiol_70_1869 |
| PubMedID: 15006820 |
| Gene_locus related to this paper: lacga-q047a5 , lacjo-q74hk5 , lacjo-q74j82 , lacjo-q74j86 , lacjo-q74k55 , lacjo-q74lt2 |
van der Kaaij H, Desiere F, Mollet B, Germond JE (2004)
L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches
Applied Environmental Microbiology
70 :1869
van der Kaaij H, Desiere F, Mollet B, Germond JE (2004)
Applied Environmental Microbiology
70 :1869