| Title : Crystal structure and characterization of esterase Est25 mutants reveal improved enantioselectivity toward (S)-ketoprofen ethyl ester - Kim_2017_Appl.Microbiol.Biotechnol_101_2333 |
| Author(s) : Kim J , Seok SH , Hong E , Yoo TH , Seo MD , Ryu Y |
| Ref : Applied Microbiology & Biotechnology , 101 :2333 , 2017 |
| Abstract : Esterases comprise a group of enzymes that catalyze the cleavage and synthesis of ester bonds. They are important in biotechnological applications owing to their enantioselectivity, regioselectivity, broad substrate specificity, and the fact that they do not require cofactors. In a previous study, we isolated the esterase Est25 from a metagenomic library. Est25 showed catalytic activity toward the (R,S)-ketoprofen ethyl ester but had low enantioselectivity toward the (S)-ketoprofen ethyl ester. Because (S)-ketoprofen has stronger anti-inflammatory effects and fewer side effects than (R)-ketoprofen, enantioselectivity of this esterase is important. In this study, we generated Est25 mutants with improved enantioselectivity toward the (S)-ketoprofen ethyl ester; improved enantioselectivity of mutants was established by analysis of their crystal structures. The enantioselectivity of mutants was influenced by substitution of Phe72 and Leu255. Substituting these residues changed the size of the binding pocket and the entrance hole that leads to the active site. The enantioselectivity of Est25 (E = 1.1 +/- 0.0) was improved in the mutants F72G (E = 1.9 +/- 0.2), L255W (E = 16.1 +/- 1.1), and F72G/L255W (E = 60.1 +/- 0.5). Finally, characterization of Est25 mutants was performed by determining the optimum reaction conditions, thermostability, effect of additives, and substrate specificity after substituting Phe72 and Leu255. |
| ESTHER : Kim_2017_Appl.Microbiol.Biotechnol_101_2333 |
| PubMedSearch : Kim_2017_Appl.Microbiol.Biotechnol_101_2333 |
| PubMedID: 27915377 |
| Gene_locus related to this paper: 9bact-q4tzq3 |
| Gene_locus related to this paper: 9bact-q4tzq3 |
Kim J, Seok SH, Hong E, Yoo TH, Seo MD, Ryu Y (2017)
Crystal structure and characterization of esterase Est25 mutants reveal improved enantioselectivity toward (S)-ketoprofen ethyl ester
Applied Microbiology & Biotechnology
101 :2333
Kim J, Seok SH, Hong E, Yoo TH, Seo MD, Ryu Y (2017)
Applied Microbiology & Biotechnology
101 :2333