Mosher_1999_Antimicrob.Agents.Chemother_43_1215

Reference

Title : Genes specific for the biosynthesis of clavam metabolites antipodal to clavulanic acid are clustered with the gene for clavaminate synthase 1 in Streptomyces clavuligerus - Mosher_1999_Antimicrob.Agents.Chemother_43_1215
Author(s) : Mosher RH , Paradkar AS , Anders C , Barton B , Jensen SE
Ref : Antimicrobial Agents & Chemotherapy , 43 :1215 , 1999
Abstract :

Portions of the Streptomyces clavuligerus chromosome flanking cas1, which encodes the clavaminate synthase 1 isoenzyme (CAS1), have been cloned and sequenced. Mutants of S. clavuligerus disrupted in cvm1, the open reading frame located immediately upstream of cas1, were constructed by a gene replacement procedure. Similar techniques were used to generate S. clavuligerus mutants carrying a deletion that encompassed portions of the two open reading frames, cvm4 and cvm5, located directly downstream of cas1. Both classes of mutants still produced clavulanic acid and cephamycin C but lost the ability to synthesize the antipodal clavam metabolites clavam-2-carboxylate, 2-hydroxymethyl-clavam, and 2-alanylclavam. These results suggested that cas1 is clustered with genes essential and specific for clavam metabolite biosynthesis. When a cas1 mutant of S. clavuligerus was constructed by gene replacement, it produced lower levels of both clavulanic acid and most of the antipodal clavams except for 2-alanylclavam. However, a double mutant of S. clavuligerus disrupted in both cas1 and cas2 produced neither clavulanic acid nor any of the antipodal clavams, including 2-alanylclavam. This outcome was consistent with the contribution of both CAS1 and CAS2 to a common pool of clavaminic acid that is shunted toward clavulanic acid and clavam metabolite biosynthesis.

PubMedSearch : Mosher_1999_Antimicrob.Agents.Chemother_43_1215
PubMedID: 10223939
Gene_locus related to this paper: strcl-a0pcj7 , strcl-CVM4

Related information

Gene_locus strcl-a0pcj7    strcl-CVM4

Citations formats

Mosher RH, Paradkar AS, Anders C, Barton B, Jensen SE (1999)
Genes specific for the biosynthesis of clavam metabolites antipodal to clavulanic acid are clustered with the gene for clavaminate synthase 1 in Streptomyces clavuligerus
Antimicrobial Agents & Chemotherapy 43 :1215

Mosher RH, Paradkar AS, Anders C, Barton B, Jensen SE (1999)
Antimicrobial Agents & Chemotherapy 43 :1215