| Title : Dipeptidyl peptidase IV of the Vespa velutina nigrithorax venom is recognized as a relevant allergen - Pretre_2022_Ann.Allergy.Asthma.Immunol__ |
| Author(s) : Pretre G , Asturias JA , Lizaso MT , Tabar AI |
| Ref : Ann Allergy Asthma Immunol , : , 2022 |
| Abstract : BACKGROUND: Vespa velutina nigrithorax (VVN), commonly known as "the Asian yellow-legged wasp," has been one of the most significant invasive species in western Europe since 2010. Currently, VVN has become the most prevalent cause of Hymenoptera anaphylaxis in north and northwestern Spain. For this reason, it is crucial to diagnose anaphylaxis cases in the acute moment for carrying out the best available treatment as soon as possible. OBJECTIVE: To achieve a complete understanding of the venom allergen composition that will help to develop efficient diagnostics and immunotherapy treatments based on this venom. METHODS: In this work, autochthonous VVN venom was obtained and characterized by SDS-PAGE, isoelectric focusing, followed by a mass spectrometry analysis. In addition, the allergenic sensitization profile of patients diagnosed with allergy to VVN in the Allergology Service of Navarra University Hospital between the years 2017-2020 was studied by immunoblotting and specific IgE (ImmunoCAP). RESULTS: Two new allergens (dipeptidyl peptidase IV and serin protease) were identified in the autochthonous VVN venom, and their identity was confirmed by LC-MS analysis. The study by ImmunoCAP using sera from 12 patients who suffered a systemic reaction after a VVN sting revealed group 5 and group 1 as predominant allergens (92% and 34%, respectively). Furthermore, the immunoblotting assay showed a recognition of the dipeptidyl peptidase IV (50%) by the sera of these patients. CONCLUSION: A serin protease and the dipeptidyl peptidase IV are components of the VVN venom, and this last one is an allergen recognized in the studied population. |
| ESTHER : Pretre_2022_Ann.Allergy.Asthma.Immunol__ |
| PubMedSearch : Pretre_2022_Ann.Allergy.Asthma.Immunol__ |
| PubMedID: 35247597 |
Pretre G, Asturias JA, Lizaso MT, Tabar AI (2022)
Dipeptidyl peptidase IV of the Vespa velutina nigrithorax venom is recognized as a relevant allergen
Ann Allergy Asthma Immunol
:
Pretre G, Asturias JA, Lizaso MT, Tabar AI (2022)
Ann Allergy Asthma Immunol
: