Paper Report for: Arai_1997_FEMS.Microbiol.Lett_152_183
Reference
Title: Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12 [published erratum appears in FEMS Microbiol Lett 1997 Sep 15;154(2):423-4] Arai T, Yusa S, Kirimura K, Usami S Ref: FEMS Microbiology Letters, 152:183, 1997 : PubMed
A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a lambda gt10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5% overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.
Arai T, Yusa S, Kirimura K, Usami S (1997) Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12 [published erratum appears in FEMS Microbiol Lett 1997 Sep 15;154(2):423-4] FEMS Microbiology Letters152: 183-8
