Paper Report for: Choi_1999_Appl.Microbiol.Biotechnol_53_7
Reference
Title: Resolution of 1,2-epoxyhexane by Rhodotorula glutinis using a two-phase membrane bioreactor Choi WJ, Choi CY, de Bont JA, Weijers CA Ref: Applied Microbiology & Biotechnology, 53:7, 1999 : PubMed
Large-scale resolution of epoxides by the yeast Rhodotorula glutinis was demonstrated in an aqueous/organic two-phase cascade membrane bioreactor. Due to the chemical instability and low solubility of epoxides in aqueous phases, an organic solvent was introduced into the reaction mixture in order to enhance the resolution of epoxide. A cascade hollow-fiber membrane bioreactor was used (1) to minimize the toxicity of organic solvents towards the epoxide hydrolase of R. glutinis, and (2) to remove inhibitory amounts of formed diol from the yeast cell containing aqueous phase. Dodecane was selected as a suitable solvent and 1,2-epoxyhexane as a model substrate. By use of this membrane bioreactor, highly concentrated (0.9 M in dodecane) enantiopure (> 98% ee) (S)-1,2-epoxyhexane (6.5 g, 30% yield) was obtained from the racemic mixture.
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Citations formats
Choi WJ, Choi CY, de Bont JA, Weijers CA (1999) Resolution of 1,2-epoxyhexane by Rhodotorula glutinis using a two-phase membrane bioreactor Applied Microbiology & Biotechnology53: 7-11
Choi WJ, Choi CY, de Bont JA, Weijers CA (1999) Applied Microbiology & Biotechnology53: 7-11
