Title : A Novel Recombinant Chlorophyllase1 from Chlamydomonas reinhardtii for the Production of Chlorophyllide Derivatives - Chou_2015_J.Agric.Food.Chem_63_9496 |
Author(s) : Chou YL , Ko CY , Yen CC , Chen LF , Shaw JF |
Ref : Journal of Agricultural and Food Chemistry , 63 :9496 , 2015 |
Abstract :
Natural chlorophyll metabolites have exhibited physiological activity in vitro. In this study, a recombinant chlorophyllase1 gene from Chlamydomonas reinhardtii (CrCLH1) was isolated and characterized. Recombinant CrCLH1 can perform chlorophyll dephytylation and produce chlorophyllide and phytol. In a transient assay, the subcellular localization of CrCLH1-green fluorescent protein was determined to be outside the chloroplast. Biochemical analyses of the activity of recombinant CrCLH1 indicated that its optimal pH value and temperature are 6.0 and 40 degreesC, respectively. Enzyme kinetic data revealed that the recombinant CrCLH1 had a higher catalytic efficiency for chlorophyll a than for chlorophyll b and bacteriochlorophyll a. According to high-performance liquid chromatography analysis of chlorophyll hydrolysis, recombinant CrCLH1 catalyzed the conversion of chlorophyll a to pheophorbide a at pH 5. Therefore, recombinant CrCLH1 can be used as a biocatalyst to produce chlorophyllide derivatives. |
PubMedSearch : Chou_2015_J.Agric.Food.Chem_63_9496 |
PubMedID: 26478543 |
Gene_locus related to this paper: chlre-a8j2s9 |
Substrate | Phaeophytin-a Bacteriochlorophyll-a Chlorophyll-a |
Gene_locus | chlre-a8j2s9 |
Chou YL, Ko CY, Yen CC, Chen LF, Shaw JF (2015)
A Novel Recombinant Chlorophyllase1 from Chlamydomonas reinhardtii for the Production of Chlorophyllide Derivatives
Journal of Agricultural and Food Chemistry
63 :9496
Chou YL, Ko CY, Yen CC, Chen LF, Shaw JF (2015)
Journal of Agricultural and Food Chemistry
63 :9496