Title: Assays of serum lipase by the BALB-DTNB method mechanized for use with discrete and continuous-flow analyzers Furukawa I, Kurooka S, Arisue K, Kohda K, Hayashi C Ref: Clinical Chemistry, 28:110, 1982 : PubMed
We successfully adapted the dimercaprol (BAL) tributyrate-5,5'-dithiobis(2-nitrobenzoic acid) method (J. Biochem. 81: 361, 1977) for assay of lipase in human serum to a discrete analyzer (the TBA 880) (I) or a continuous-flow analyzer (AutoAnalyzer, Type II) (II). In both, BAL-tributyrate is used as substrate, in combination with serum esterase inhibitors and a chromogenic reagent for the SH group of the liberated BAL. Serum lipase activities of patients with pancreatic diseases, measured at 90 or 40 samples per hour by I or II, respectively, correlated well with those measured by the corresponding manual method or by Kaplan's radioassay (Anal. Biochem. 33: 213, 1970). The correlation coefficients were all greater than 0.95, and the coefficients of variation were less than 8%, showing the practical usefulness of these procedures.
Furukawa I, Kurooka S, Arisue K, Kohda K, Hayashi C (1982) Assays of serum lipase by the BALB-DTNB method mechanized for use with discrete and continuous-flow analyzers Clinical Chemistry28: 110-3
Furukawa I, Kurooka S, Arisue K, Kohda K, Hayashi C (1982) Clinical Chemistry28: 110-3
