Title : [The effect of pH and reversible inhibitors on decarbamylation of acetylcholinesterase] - Kugusheva_1991_Ukr.Biokhim.Zh.(1978)_63_29 |
Author(s) : Kugusheva LI , Rozengart VI , Sherstobitov OE |
Ref : Ukr Biokhim Zh (1978) , 63 :29 , 1991 |
Abstract :
Decarbamylation rate of membrane-bound methyl- and dimethyl-carbamylated acetylcholinesterase of human erythrocytes and bovine brain is reliably 1.1-1.6 times lower than that of the soluble enzyme. Such reversible inhibitors as tacrine (of non-competition action), ambenonium (mixed action) and galanthamine (competitive type of action) decelerate the decarbamylation rate of acetylcholinesterase. At pH 6 tacrine inhibits the reduction rate of soluble acetylcholinesterase activity of human erythrocytes more intensively than that of membrane-bound acetylcholinesterase. No differences in decarbamylation rate were found for the both forms of the enzyme at pH 8. Tacrine, a non-competitive inhibitor in concentrations below the inhibition constant (Ki = 1.4 x 10(-7) M) exerts the most intensive effect on the decarbamylation rate of methyl- and dimethylcarbamylated acetylcholinesterase of the mouse brain, while ambenonium and galanthamine in concentrations much (tens times) exceeding their Ki (3.1 x 10(-10) M and 4.4 x 10(-7) M, respectively) provide a decrease of the decarbamylation rate. |
PubMedSearch : Kugusheva_1991_Ukr.Biokhim.Zh.(1978)_63_29 |
PubMedID: 1926583 |
Kugusheva LI, Rozengart VI, Sherstobitov OE (1991)
[The effect of pH and reversible inhibitors on decarbamylation of acetylcholinesterase]
Ukr Biokhim Zh (1978)
63 :29
Kugusheva LI, Rozengart VI, Sherstobitov OE (1991)
Ukr Biokhim Zh (1978)
63 :29