Title : Absence of cardiac lipid accumulation in transgenic mice with heart-specific HSL overexpression - Suzuki_2001_Am.J.Physiol.Endocrinol.Metab_281_E857 |
Author(s) : Suzuki J , Shen WJ , Nelson BD , Patel S , Veerkamp JH , Selwood SP , Murphy GM, Jr. , Reaven E , Kraemer FB |
Ref : American Journal of Physiology Endocrinol Metab , 281 :E857 , 2001 |
Abstract :
Hormone-sensitive lipase (HSL) hydrolyzes triglyceride (TG) in adipose tissue. HSL is also expressed in heart. To explore the actions of cardiac HSL, heart-specific, tetracycline (Tc)-controlled HSL-overexpressing mice were generated. Tc-responsive element-HSL transgenic (Tg) mice were generated and crossed with myosin heavy chain (MHC)alpha-tTA Tg mice, which express the Tc-responsive transactivator (tTA) in the heart. The double-Tg mice (MHC-HSL) were maintained with doxycycline (Dox) to suppress Tg HSL. Upon removal of Dox, cardiac HSL activity and protein increased 12- and 8-fold, respectively, and the expression was heart specific. Although cardiac TG content increased twofold in control mice after an overnight fast, it did not increase in HSL-induced mice. Electron microscopy showed numerous lipid droplets in the myocardium of fasted control mice, whereas fasted HSL-induced mice showed virtually no droplets. Microarray analysis showed altered expression of cardiac genes for fatty acid oxidation, transcription factors, signaling molecules, cytoskeletal proteins, and histocompatibility antigens in HSL-induced mice. Thus cardiac HSL plays a role in controlling accumulation of triglyceride droplets and can affect the expression of a number of cardiac genes. |
PubMedSearch : Suzuki_2001_Am.J.Physiol.Endocrinol.Metab_281_E857 |
PubMedID: 11551864 |
Suzuki J, Shen WJ, Nelson BD, Patel S, Veerkamp JH, Selwood SP, Murphy GM, Jr., Reaven E, Kraemer FB (2001)
Absence of cardiac lipid accumulation in transgenic mice with heart-specific HSL overexpression
American Journal of Physiology Endocrinol Metab
281 :E857
Suzuki J, Shen WJ, Nelson BD, Patel S, Veerkamp JH, Selwood SP, Murphy GM, Jr., Reaven E, Kraemer FB (2001)
American Journal of Physiology Endocrinol Metab
281 :E857