| Title : Comparison of Five Protein Engineering Strategies for Stabilizing an alpha\/beta-Hydrolase - Jones_2017_Biochemistry_56_6521 |
| Author(s) : Jones BJ , Lim HY , Huang J , Kazlauskas RJ |
| Ref : Biochemistry , 56 :6521 , 2017 |
| Abstract : A review of the previous stabilization of alpha/beta-hydrolase fold enzymes revealed many different strategies, but no comparison of strategies on the same enzyme. For this reason, we compared five strategies to identify stabilizing mutations in a model alpha/beta-hydrolase fold enzyme, salicylic acid binding protein 2, to reversible denaturation by urea and to irreversible denaturation by heat. The five strategies included one location agnostic approach (random mutagenesis using error-prone polymerase chain reaction), two structure-based approaches [computational design (Rosetta, FoldX) and mutation of flexible regions], and two sequence-based approaches (addition of proline at locations where a more stable homologue has proline and mutation to consensus). All strategies identified stabilizing mutations, but the best balance of success rate, degree of stabilization, and ease of implementation was mutation to consensus. A web-based automated program that predicts substitutions needed to mutate to consensus is available at http://kazlab.umn.edu . |
| ESTHER : Jones_2017_Biochemistry_56_6521 |
| PubMedSearch : Jones_2017_Biochemistry_56_6521 |
| PubMedID: 29087185 |
| Gene_locus related to this paper: nicta-SABP2 |
| Gene_locus related to this paper: nicta-SABP2 |
Jones BJ, Lim HY, Huang J, Kazlauskas RJ (2017)
Comparison of Five Protein Engineering Strategies for Stabilizing an alpha\/beta-Hydrolase
Biochemistry
56 :6521
Jones BJ, Lim HY, Huang J, Kazlauskas RJ (2017)
Biochemistry
56 :6521