Kazlauskas RJ

General

Full name : Kazlauskas Romas J

First name : Romas J Kazlauskas

Mail : University of Minnesota, Department of Biochemistry, Molecular Biology & Biophysics and The Biotechnology Institute, 1479 Gortner Avenue, Saint Paul, MN 55108

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Country : USA

Email : rjk@umn.edu

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References (41)

Title : Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity - Jones_2020_PLoS.One_15_e0235341
Author(s) : Jones BJ , Evans RL, 3rd , Mylrea NJ , Chaudhury D , Luo C , Guan B , Pierce CT , Gordon WR , Wilmot CM , Kazlauskas RJ
Ref : PLoS ONE , 15 :e0235341 , 2020
Abstract :
PubMedSearch : Jones_2020_PLoS.One_15_e0235341
PubMedID: 32603354
Gene_locus related to this paper: 9zzzz-5TDX

Title : Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism - Jones_2017_ACS.Catal_7_4221
Author(s) : Jones BJ , Bata Z , Kazlauskas RJ
Ref : ACS Catal , 7 :4221 , 2017
Abstract :
PubMedSearch : Jones_2017_ACS.Catal_7_4221
PubMedID: 28798888
Gene_locus related to this paper: arath-HNL , hevbr-hnl

Title : Comparison of Five Protein Engineering Strategies for Stabilizing an alpha\/beta-Hydrolase - Jones_2017_Biochemistry_56_6521
Author(s) : Jones BJ , Lim HY , Huang J , Kazlauskas RJ
Ref : Biochemistry , 56 :6521 , 2017
Abstract :
PubMedSearch : Jones_2017_Biochemistry_56_6521
PubMedID: 29087185
Gene_locus related to this paper: nicta-SABP2

Title : Evolution of a Catalytic Mechanism - Rauwerdink_2016_Mol.Biol.Evol_33_971
Author(s) : Rauwerdink A , Lunzer M , Devamani T , Jones B , Mooney J , Zhang ZJ , Xu JH , Kazlauskas RJ , Dean AM
Ref : Molecular Biology Evolution , 33 :971 , 2016
Abstract :
PubMedSearch : Rauwerdink_2016_Mol.Biol.Evol_33_971
PubMedID: 26681154
Gene_locus related to this paper: 9zzzz-5TDX , hevbr-hnl

Title : Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases - Devamani_2016_J.Am.Chem.Soc_138_1046
Author(s) : Devamani T , Rauwerdink AM , Lunzer M , Jones BJ , Mooney JL , Tan MA , Zhang ZJ , Xu JH , Dean AM , Kazlauskas RJ
Ref : Journal of the American Chemical Society , 138 :1046 , 2016
Abstract :
PubMedSearch : Devamani_2016_J.Am.Chem.Soc_138_1046
PubMedID: 26736133

Title : Stabilization of an alpha\/beta-Hydrolase by Introducing Proline Residues: Salicylic Acid Binding Protein 2 from Tobacco -
Author(s) : Huang J , Jones BJ , Kazlauskas RJ
Ref : Biochemistry , 54 :4330 , 2015
PubMedID: 26110207
Gene_locus related to this paper: nicta-SABP2

Title : How the Same Core Catalytic Machinery Catalyzes 17 Different Reactions: the Serine-Histidine-Aspartate Catalytic Triad of alpha\/beta-Hydrolase Fold Enzymes - Rauwerdink_2015_ACS.Catal_5_6153
Author(s) : Rauwerdink A , Kazlauskas RJ
Ref : ACS Catal , 5 :6153 , 2015
Abstract :
PubMedSearch : Rauwerdink_2015_ACS.Catal_5_6153
PubMedID: 28580193

Title : Increasing the reaction rate of hydroxynitrile lyase from Hevea brasiliensis toward mandelonitrile by copying active site residues from an esterase that accepts aromatic esters - von Langermann_2014_Chembiochem_15_1931
Author(s) : von Langermann J , Nedrud DM , Kazlauskas RJ
Ref : Chembiochem , 15 :1931 , 2014
Abstract :
PubMedSearch : von Langermann_2014_Chembiochem_15_1931
PubMedID: 25044660

Title : Uncovering divergent evolution of alpha\/beta-hydrolases: a surprising residue substitution needed to convert hydroxynitrile lyase into an esterase - Nedrud_2014_Chem.Sci_5_4265
Author(s) : Nedrud DM , Lin H , Lopez G , Padhi SK , Legatt GA , Kazlauskas RJ
Ref : Chem Sci , 5 :4265 , 2014
Abstract :
PubMedSearch : Nedrud_2014_Chem.Sci_5_4265
PubMedID: 25346843
Gene_locus related to this paper: hevbr-hnl , nicta-SABP2

Title : New structural motif for carboxylic acid perhydrolases - Yin_2013_Chemistry_19_3037
Author(s) : Yin DT , Purpero VM , Fujii R , Jing Q , Kazlauskas RJ
Ref : Chemistry , 19 :3037 , 2013
Abstract :
PubMedSearch : Yin_2013_Chemistry_19_3037
PubMedID: 23325572
Gene_locus related to this paper: psefl-este

Title : Engineering the third wave of biocatalysis - Bornscheuer_2012_Nature_485_185
Author(s) : Bornscheuer UT , Huisman GW , Kazlauskas RJ , Lutz S , Moore JC , Robins K
Ref : Nature , 485 :185 , 2012
Abstract :
PubMedSearch : Bornscheuer_2012_Nature_485_185
PubMedID: 22575958

Title : Revised molecular basis of the promiscuous carboxylic acid perhydrolase activity in serine hydrolases - Yin_2012_Chemistry_18_8130
Author(s) : Yin DT , Kazlauskas RJ
Ref : Chemistry , 18 :8130 , 2012
Abstract :
PubMedSearch : Yin_2012_Chemistry_18_8130
PubMedID: 22618813

Title : Protein engineering of alpha\/beta-hydrolase fold enzymes - Jochens_2011_Chembiochem_12_1508
Author(s) : Jochens H , Hesseler M , Stiba K , Padhi SK , Kazlauskas RJ , Bornscheuer UT
Ref : Chembiochem , 12 :1508 , 2011
Abstract :
PubMedSearch : Jochens_2011_Chembiochem_12_1508
PubMedID: 21506229
Gene_locus related to this paper: canan-lipasA

Title : Survey of protein engineering strategies - Bornscheuer_2011_Curr.Protoc.Protein.Sci_Chapter 26_Unit26 7
Author(s) : Bornscheuer U , Kazlauskas RJ
Ref : Curr Protoc Protein Science , Chapter 26 :Unit26 7 , 2011
Abstract :
PubMedSearch : Bornscheuer_2011_Curr.Protoc.Protein.Sci_Chapter 26_Unit26 7
PubMedID: 22045562

Title : Improved pretreatment of lignocellulosic biomass using enzymatically-generated peracetic acid - Yin_2011_Bioresour.Technol_102_5183
Author(s) : Yin DT , Jing Q , Aldajani WW , Duncan S , Tschirner U , Schilling J , Kazlauskas RJ
Ref : Bioresour Technol , 102 :5183 , 2011
Abstract :
PubMedSearch : Yin_2011_Bioresour.Technol_102_5183
PubMedID: 21345668

Title : Insights into the molecular basis of chiral acid recognition by Candida rugosa lipase from an X-ray crystal structure of a bound phosphonate transition state analog - Colton_2011_Adv.Synth.Catal_353_2529
Author(s) : Colton IJ , Yin DT , Grochulski P , Kazlauskas RJ
Ref : Adv Synth Catal , 353 :2529 , 2011
Abstract :
PubMedSearch : Colton_2011_Adv.Synth.Catal_353_2529
PubMedID:
Gene_locus related to this paper: canru-1lipa

Title : Different active-site loop orientation in serine hydrolases versus acyltransferases - Jiang_2011_Chembiochem_12_768
Author(s) : Jiang Y , Morley KL , Schrag JD , Kazlauskas RJ
Ref : Chembiochem , 12 :768 , 2011
Abstract :
PubMedSearch : Jiang_2011_Chembiochem_12_768
PubMedID: 21351219
Gene_locus related to this paper: psefl-este

Title : Increased saccharification yields from aspen biomass upon treatment with enzymatically generated peracetic acid - Duncan_2010_Appl.Biochem.Biotechnol_160_1637
Author(s) : Duncan S , Jing Q , Katona A , Kazlauskas RJ , Schilling J , Tschirner U , Aldajani WW
Ref : Appl Biochem Biotechnol , 160 :1637 , 2010
Abstract :
PubMedSearch : Duncan_2010_Appl.Biochem.Biotechnol_160_1637
PubMedID: 19484411

Title : Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions - Padhi_2010_Chem.Biol_17_863
Author(s) : Padhi SK , Fujii R , Legatt GA , Fossum SL , Berchtold R , Kazlauskas RJ
Ref : Chemical Biology , 17 :863 , 2010
Abstract :
PubMedSearch : Padhi_2010_Chem.Biol_17_863
PubMedID: 20797615
Gene_locus related to this paper: hevbr-hnl , nicta-SABP2

Title : Switching catalysis from hydrolysis to perhydrolysis in Pseudomonas fluorescens esterase - Yin_2010_Biochemistry_49_1931
Author(s) : Yin DL , Bernhardt P , Morley KL , Jiang Y , Cheeseman JD , Purpero V , Schrag JD , Kazlauskas RJ
Ref : Biochemistry , 49 :1931 , 2010
Abstract :
PubMedSearch : Yin_2010_Biochemistry_49_1931
PubMedID: 20112920
Gene_locus related to this paper: psefl-este

Title : Finding better protein engineering strategies -
Author(s) : Kazlauskas RJ , Bornscheuer UT
Ref : Nat Chemical Biology , 5 :526 , 2009
PubMedID: 19620988

Title : Converting an esterase into an epoxide hydrolase - Jochens_2009_Angew.Chem.Int.Ed.Engl_48_3532
Author(s) : Jochens H , Stiba K , Savile C , Fujii R , Yu JG , Gerassenkov T , Kazlauskas RJ , Bornscheuer UT
Ref : Angew Chem Int Ed Engl , 48 :3532 , 2009
Abstract :
PubMedSearch : Jochens_2009_Angew.Chem.Int.Ed.Engl_48_3532
PubMedID: 19350592
Gene_locus related to this paper: psefl-este

Title : Molecular basis for the stereoselective ammoniolysis of N-alkyl aziridine-2-carboxylates catalyzed by Candida antarctica lipase B - Park_2009_Chembiochem_10_2213
Author(s) : Park JH , Ha HJ , Lee WK , Genereux-Vincent T , Kazlauskas RJ
Ref : Chembiochem , 10 :2213 , 2009
Abstract :
PubMedSearch : Park_2009_Chembiochem_10_2213
PubMedID: 19672908

Title : Determination of absolute configuration of secondary alcohols using lipase-catalyzed kinetic resolutions - Jing_2008_Chirality_20_724
Author(s) : Jing Q , Kazlauskas RJ
Ref : Chirality , 20 :724 , 2008
Abstract :
PubMedSearch : Jing_2008_Chirality_20_724
PubMedID: 18278808

Title : Enzymatic synthesis of poly(hydroxyalkanoates) in ionic liquids - Gorke_2007_J.Biotechnol_132_306
Author(s) : Gorke JT , Okrasa K , Louwagie A , Kazlauskas RJ , Srienc F
Ref : J Biotechnol , 132 :306 , 2007
Abstract :
PubMedSearch : Gorke_2007_J.Biotechnol_132_306
PubMedID: 17543408

Title : Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity - Nisole_2006_Protein.Expr.Purif_46_274
Author(s) : Nisole A , Lussier FX , Morley KL , Shareck F , Kazlauskas RJ , Dupont C , Pelletier JN
Ref : Protein Expr Purif , 46 :274 , 2006
Abstract :
PubMedSearch : Nisole_2006_Protein.Expr.Purif_46_274
PubMedID: 16256365

Title : Remote interactions explain the unusual regioselectivity of lipase from Pseudomonas cepacia toward the secondary hydroxyl of 2'-deoxynucleosides - Lavandera_2006_Chembiochem_7_693
Author(s) : Lavandera I , Fernandez S , Magdalena J , Ferrero M , Grewal H , Savile CK , Kazlauskas RJ , Gotor V
Ref : Chembiochem , 7 :693 , 2006
Abstract :
PubMedSearch : Lavandera_2006_Chembiochem_7_693
PubMedID: 16491501

Title : Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations - Park_2005_Chem.Biol_12_45
Author(s) : Park S , Morley KL , Horsman GP , Holmquist M , Hult K , Kazlauskas RJ
Ref : Chemical Biology , 12 :45 , 2005
Abstract :
PubMedSearch : Park_2005_Chem.Biol_12_45
PubMedID: 15664514

Title : Molecular basis of perhydrolase activity in serine hydrolases -
Author(s) : Bernhardt P , Hult K , Kazlauskas RJ
Ref : Angew Chem Int Ed Engl , 44 :2742 , 2005
PubMedID: 15803517

Title : Enhancing catalytic promiscuity for biocatalysis - Kazlauskas_2005_Curr.Opin.Chem.Biol_9_195
Author(s) : Kazlauskas RJ
Ref : Curr Opin Chemical Biology , 9 :195 , 2005
Abstract :
PubMedSearch : Kazlauskas_2005_Curr.Opin.Chem.Biol_9_195
PubMedID: 15811805

Title : Mirror-image packing in enantiomer discrimination molecular basis for the enantioselectivity of B.cepacia lipase toward 2-methyl-3-phenyl-1-propanol - Mezzetti_2005_Chem.Biol_12_427
Author(s) : Mezzetti A , Schrag JD , Cheong CS , Kazlauskas RJ
Ref : Chemical Biology , 12 :427 , 2005
Abstract :
PubMedSearch : Mezzetti_2005_Chem.Biol_12_427
PubMedID: 15850979
Gene_locus related to this paper: burce-lipaa

Title : An inverse substrate orientation for the regioselective acylation of 3',5'-diaminonucleosides catalyzed by Candida antarctica lipase B? - Lavandera_2005_Chembiochem_6_1381
Author(s) : Lavandera I , Fernandez S , Magdalena J , Ferrero M , Kazlauskas RJ , Gotor V
Ref : Chembiochem , 6 :1381 , 2005
Abstract :
PubMedSearch : Lavandera_2005_Chembiochem_6_1381
PubMedID: 15977272
Gene_locus related to this paper: canar-LipB

Title : Structure of an aryl esterase from Pseudomonas fluorescens - Cheeseman_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_1237
Author(s) : Cheeseman JD , Tocilj A , Park S , Schrag JD , Kazlauskas RJ
Ref : Acta Crystallographica D Biol Crystallogr , 60 :1237 , 2004
Abstract :
PubMedSearch : Cheeseman_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_1237
PubMedID: 15213385
Gene_locus related to this paper: psefl-este

Title : Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate - Horsman_2003_Chemistry_9_1933
Author(s) : Horsman GP , Liu AM , Henke E , Bornscheuer UT , Kazlauskas RJ
Ref : Chemistry , 9 :1933 , 2003
Abstract :
PubMedSearch : Horsman_2003_Chemistry_9_1933
PubMedID: 12740839
Gene_locus related to this paper: psefl-este

Title : Improved preparation and use of room-temperature ionic liquids in lipase-catalyzed enantio- and regioselective acylations - Park_2001_J.Org.Chem_66_8395
Author(s) : Park S , Kazlauskas RJ
Ref : J Org Chem , 66 :8395 , 2001
Abstract :
PubMedSearch : Park_2001_J.Org.Chem_66_8395
PubMedID: 11735517

Title : Molecular basis for enantioselectivity of lipase from Chromobacterium viscosum toward the diesters of 2,3-dihydro-3-(4'-hydroxyphenyl)-1,1,3-trimethyl-1H-inden-5-ol - Gascoyne_2001_J.Org.Chem_66_3041
Author(s) : Gascoyne DG , Finkbeiner HL , Chan KP , Gordon JL , Stewart KR , Kazlauskas RJ
Ref : J Org Chem , 66 :3041 , 2001
Abstract :
PubMedSearch : Gascoyne_2001_J.Org.Chem_66_3041
PubMedID: 11325269
Gene_locus related to this paper: burgl-lipas

Title : Molecular Basis for Enantioselectivity of Lipase from Pseudomonas cepacia toward Primary Alcohols. Modeling, Kinetics, and Chemical Modification of Tyr29 to Increase or Decrease Enantioselectivity - Tuomi_1999_J.Org.Chem_64_2638
Author(s) : Tuomi WV , Kazlauskas RJ
Ref : J Org Chem , 64 :2638 , 1999
Abstract :
PubMedSearch : Tuomi_1999_J.Org.Chem_64_2638
PubMedID: 11674331
Gene_locus related to this paper: burce-lipaa

Title : Analogs of reaction intermediates identify a unique substrate binding site in Candida rugosa lipase - Grochulski_1994_Biochemistry_33_3494
Author(s) : Grochulski P , Bouthillier F , Kazlauskas RJ , Serreqi AN , Schrag JD , Ziomek E , Cygler M
Ref : Biochemistry , 33 :3494 , 1994
Abstract :
PubMedSearch : Grochulski_1994_Biochemistry_33_3494
PubMedID: 8142346
Gene_locus related to this paper: canru-1lipa

Title : A structural basis for the chiral preferences of lipases -
Author(s) : Cygler M , Grochulski P , Kazlauskas RJ , Schrag JD , Bouthillier F , Rubin B , Serreqi AN , Gupta AK
Ref : Journal of the American Chemical Society , 116 :3180 , 1994
PubMedID:
Gene_locus related to this paper: canru-1lipa

Title : Elucidating structure-mechanism relationships in lipases: prospects for predicting and engineering catalytic properties - Kazlauskas_1994_Trends.Biotechnol_12_464
Author(s) : Kazlauskas RJ
Ref : Trends Biotechnol , 12 :464 , 1994
Abstract :
PubMedSearch : Kazlauskas_1994_Trends.Biotechnol_12_464
PubMedID: 7765546

Title : Biocatalysis--becoming more predictable and selective -
Author(s) : Kazlauskas RJ
Ref : Trends Biotechnol , 11 :439 , 1993
PubMedID: 7764396