Covino S

References (4)

Title : The Fibrinogen-like Domain of ANGPTL3 Facilitates Lipolysis in 3T3-L1 Cells by Activating the Intracellular Erk Pathway - Bini_2022_Biomolecules_12_
Author(s) : Bini S , Pecce V , Di Costanzo A , Polito L , Ghadiri A , Minicocci I , Tambaro F , Covino S , Arca M , D'Erasmo L
Ref : Biomolecules , 12 : , 2022
Abstract : BACKGROUND: ANGPTL3 stimulates lipolysis in adipocytes, but the underlying molecular mechanism is yet unknown. The C-terminal fibrinogen-like domain of ANGPTL3 (ANGPTL3-Fld) activates the AKT pathway in endothelial cells. Hence, we evaluated whether ANGPTL3-Fld stimulates lipolysis in adipocytes through the MAPK kinase pathway. MATERIALS AND METHODS: 3T3-L1 adipocytes were treated with isoproterenol (ISO), ANGPTL3-Fld, or both. Lipolysis was evaluated through the release of free fatty acids (FFAs) in the culture medium. The activation status of intracellular kinases was evaluated with and without the inhibition of the BRAF-ERK arm of the MAPK pathway. RESULTS: ANGPTL3-Fld alone was not able to activate lipolysis, while the combination of ANGPTL3-Fld and ISO determined a 10-fold enrichment of the FFA concentration in the culture medium with an incremental effect (twofold) when compared with ISO alone. ANGPTL3-Fld alone inhibited hormone-sensitive lipase (HSL), whereas the treatment with ISO induced the activation of HSL. The net balance of ANGPTL3-Fld and ISO cotreatment resulted in HSL activation. The results indicate that ANGPTL3-Fld generated an intracellular activation signal involving the MAPK-ERK pathway, possibly through the PDGFRbeta-PLCgamma-AMPK axis. CONCLUSION: ANGPTL3-Fld appears to act as a facilitator of lipolysis in adipocytes, and this effect was driven by a signal mediated by a pathway that is different from the canonical beta-adrenergic stimulus.
ESTHER : Bini_2022_Biomolecules_12_
PubMedSearch : Bini_2022_Biomolecules_12_
PubMedID: 35454174

Title : Bridelia speciosa Mull.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf - Mahomoodally_2020_Antioxidants.(Basel)_9_
Author(s) : Mahomoodally MF , Sinan KI , Bene K , Zengin G , Orlando G , Menghini L , Veschi S , Chiavaroli A , Recinella L , Brunetti L , Leone S , Angelini P , Hubka V , Covino S , Venanzoni R , Picot-Allain MCN , Lellis L , Cama A , Cziaky Z , Jeko J , Ferrante C
Ref : Antioxidants (Basel) , 9 : , 2020
Abstract : Bridelia species have been used in traditional African medicine for the management of diverse human ailments. In the current work, the detailed phytochemical profiles of the extracts of the stem bark of B. speciosa were evaluated and the antioxidant and enzyme inhibitory properties of the extracts were assessed. The anti-bacterial and anti-mycotic effects of the extracts were evaluated against selected pathogen strains. Additionally, the anti-proliferative effects were studied on the liver cancer HepG2 cell line. Finally, the putative protective effects were assessed on isolated rat liver that was challenged with lipopolysaccharide (LPS). The results revealed the presence of 36 compounds in the ethyl acetate extract, 44 in the methanol extract, and 38 in the water extract. Overall, the methanol extract showed the highest antioxidant activity, particularly in LPS-stimulated rat liver. Additionally, this extract exerted the highest antimycotic effect on C. albicans, whereas the water extract showed a promising anti-proliferative effect on liver cancer HepG2 cells. The methanol extract was also the most active as enzyme inhibitor, against acetylcholinesterase and butyrylcholinesterase. The current study appraises the antioxidant and enzyme inhibition properties of B. speciosa methanol extract and showed that this specie could be a promising source of biologically active phytochemicals, with potential health uses.
ESTHER : Mahomoodally_2020_Antioxidants.(Basel)_9_
PubMedSearch : Mahomoodally_2020_Antioxidants.(Basel)_9_
PubMedID: 32024319

Title : Chemical profiling and pharmaco-toxicological activity of Origanum sipyleum extracts: Exploring for novel sources for potential therapeutic agents - Zengin_2019_J.Food.Biochem__e13003
Author(s) : Zengin G , Ferrante C , Orlando G , Zheleva-Dimitrova D , Gevrenova R , Recinella L , Chiavaroli A , Leone S , Brunetti L , Aumeeruddy MZ , Aktumsek A , Mahomoodally MF , Angelini P , Covino S , Venanzoni R , Tirillini B , Menghini L
Ref : J Food Biochem , :e13003 , 2019
Abstract : The phytochemical, antiradical, and enzyme inhibition profile of three solvent extracts (ethyl acetate, methanol, water) of Origanum sipyleum were assessed. We also performed a pharmacological study in order to explore protective effects induced by extracts in inflamed colon. LC-MS analysis revealed that the extracts contained different classes of phenolics. The aqueous extract showed the highest antioxidant and acetylcholinesterase (AChE) inhibitory effects. Total phenol and flavonoid contents were highest in aqueous and ethyl acetate extract, respectively. All extracts were effective in reducing colon pro-oxidant and pro-inflammatory biomarkers. The extracts revealed also able to inhibit fungal and bacterial species involved in ulcerative colitis, including Candida albicans, Candida tropicalis, Staphylococcus aureus, and Staphylococcus thyphimurium. Finally, we also showed the antiproliferative effects exerted by the EA extracts on human colon cancer HCT116 cell line. Concluding, our results indicated that O. sipyleum extracts displayed promising therapeutic properties which warrants further validation. PRACTICAL APPLICATIONS: The present phytochemical and biological studies, including antioxidant, anti-inflammatory, and antimicrobic assessments, showed significant protective effects exerted by O. sipyleum extracts in an experimental model of ulcerative colitis. The results are intriguing and suggest potential applications O. sipyleum extracts as sources of natural agents for the management of clinical symptoms related to ulcerative colitis, characterized by increased burden of oxidative stress and microbiome dysbiosis.
ESTHER : Zengin_2019_J.Food.Biochem__e13003
PubMedSearch : Zengin_2019_J.Food.Biochem__e13003
PubMedID: 31393014

Title : Comprehensive approaches on the chemical constituents and pharmacological properties of flowers and leaves of American basil (Ocimum americanum L) - Zengin_2019_Food.Res.Int_125_108610
Author(s) : Zengin G , Ferrante C , Gnapi DE , Sinan KI , Orlando G , Recinella L , Diuzheva A , Jeko J , Cziaky Z , Chiavaroli A , Leone S , Brunetti L , Picot-Allain C , Mahomoodally MF , Angelini P , Covino S , Venanzoni R , Tirillini B , Menghini L
Ref : Food Res Int , 125 :108610 , 2019
Abstract : Ocimum americanum L. (Lamiaceae) is a common food condiment and also used in traditional medicine in the management of several human diseases. Nonetheless, there has been no effort to delineate the biological and phytochemical profiles of leaves and flowers prepared by different extractive solvents (ethyl acetate, methanol (MeOH), and water). The pharmacological potential of O. americanum extracts on pro-oxidant/pro-inflammatory mediators in rat colon specimens treated with lipopolysaccharide was investigated. In parallel, the inhibitory effects of the extracts on fungal and bacterial strains involved in ulcerative colitis were studied. Qualitative phytochemical analysis showed the presence of phenols, flavonoids, and tannins. Water extracts of flowers and leaves showed strong reducing and radicals scavenging potential. Both MeOH and ethyl acetate extracts of the leaves and flowers were able to inhibit acetylcholinesterase, butyrylcholinesterase, and tyrosinase. All the extracts inhibited the selected bacterial and fungal strains, while only ethyl acetate flower extract displayed antioxidant/anti-inflammatory effects in rat colon. The water and MeOH extracts stimulated colon lactate dehydrogenase (LDH) and serotonin (5-HT) and induced spontaneous migration of HCT116 cells. Future investigations should focus on the biological activity of isolated phytochemicals from the leaves and flowers of O. americanum, in order to clarify the mechanism(s) of action substantiating the observed pharmacological properties.
ESTHER : Zengin_2019_Food.Res.Int_125_108610
PubMedSearch : Zengin_2019_Food.Res.Int_125_108610
PubMedID: 31554064