Heilemann M

References (1)

Title : Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy - Kompa_2023_J.Am.Chem.Soc_145_3075
Author(s) : Kompa J , Bruins J , Glogger M , Wilhelm J , Frei MS , Tarnawski M , x , Este E , Heilemann M , Hiblot J , Johnsson K
Ref : Journal of the American Chemical Society , 145 :3075 , 2023
Abstract : The specific and covalent labeling of the protein HaloTag with fluorescent probes in living cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the probe to HaloTag precludes imaging applications that require transient binding of the probe and comes with the risk of irreversible photobleaching. Here, we introduce exchangeable ligands for fluorescence labeling of HaloTag (xHTLs) that reversibly bind to HaloTag and that can be coupled to rhodamines of different colors. In stimulated emission depletion (STED) microscopy, probe exchange of xHTLs allows imaging with reduced photobleaching as compared to covalent HaloTag labeling. Transient binding of fluorogenic xHTLs to HaloTag fusion proteins enables points accumulation for imaging in nanoscale topography (PAINT) and MINFLUX microscopy. We furthermore introduce pairs of xHTLs and HaloTag mutants for dual-color PAINT and STED microscopy. xHTLs thus open up new possibilities in imaging across microscopy platforms for a widely used labeling approach.
ESTHER : Kompa_2023_J.Am.Chem.Soc_145_3075
PubMedSearch : Kompa_2023_J.Am.Chem.Soc_145_3075
PubMedID: 36716211
Gene_locus related to this paper: rhoso-halo1