rhoso-halo1
Rhodococcus rhodochrous (Pseudomonas pavonaceae), Rhodococcus sp., Mycobacterium sp haloalkane dehalogenase (EC 3.8.1.5) DhaA HaloTag (hyperstable variant DhaA115)
Comment
Other strains: Rhodococcus rhodochrous (NCIMB 13064)\; Rhodococcus sp. (strain TDTM0003)\; Mycobacterium sp (strain GP1)\; Pseudomonas pavonaceae) encoded on plasmid. The HaloTag is Rhodococcus rhodochrous haloalkane dehalogenase, which has a genetically modified active site, which specifically binds the reactive chloroalkane linker and has an increased rate of ligand binding. HaloTagged fusion proteins can be expressed easily and allow enzymatic assays, cellular imaging, protein arrays, determination of sub-cellular localization.(HaloTag L47V, S58T, D78G, Y87F, L88M, C128F, A155T, E160K, A167V, A172T, K175M, C176G, K195N, A224E, N227D, E257K, T264A, H272N, Y273L) Some of the most recent structures of proteins with halotag are not included in ESTHER as the tag is only a tool and not analyzed as an alpha\/beta hydolase: see for example 7KTS 7KTR. DhaA115 thermostable multiple-point mutants || haloalkane dehalogenase Pseudomonas pavonaceae differs only with two aa of Rhodococcus haloalkane dehalogenase. Also identical to Mycobacterium sp (same paper)
Relationship
Family : Haloalkane_dehalogenase-HLD2
Block : X
Position in NCBI Life Tree : Rhodococcus rhodochrous
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Actinobacteria [phylum] N E > Actinobacteria [class] N E > Corynebacteriales N E > Nocardiaceae N E > Rhodococcus N E > Rhodococcus rhodochrous N EMolecular evidence
No mutation
1BN6 - Rhodococcus haloalkane dehalogenase
1BN7 - Rhodococcus haloalkane dehalogenase in complex with acetate
1CQW - Rhodococcus Sp. Haloalcane dehalogenase cocrystal Nai
2V9Z - Structure of the Rhodococcus haloalkane dehalogenase mutant with enhanced enantioselectivity
3FBW - Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA mutant C176Y
3FWH - Structure of haloalkane dehalogenase mutant Dha15 (I135F\/C176Y) from Rhodococcus rhodochrous
3G9X - Structure of haloalkane dehalogenase DhaA14 mutant I135F from Rhodococcus rhodochrous
3RK4 - Structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant DhaA31
3SK0 - Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA mutant DhaA12
4E46 - Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA in complex with 2-propanol
4F5Z - Crystal structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant (L95V, A172V)
4F60 - Crystal structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant (T148L, G171Q, A172V, C176F)
4FWB - Structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant DhaA31 in complex with 1, 2, 3 - trichloropropane SUPERSEDES 3RLT
4HZG - Structure of haloalkane dehalogenase DhaA from Rhodococcus rhodochrous
4KAA - Crystal structure of the halotag2 protein at the resolution 2.3A, Northeast Structural Genomics Consortium (NESG) target OR150
4KAC - X-Ray Structure of the complex HaloTag2 with HALTS. Northeast Structural Genomics Consortium (NESG) Target OR150.
4KAF - Crystal Structure of Haloalkane dehalogenase HaloTag7 at the resolution 1.5A, Northeast Structural Genomics Consortium (NESG) Target OR151
4KAJ - X-Ray Structure of the complex of Haloalkane dehalogenase HaloTag7 with HALTS, Northeast Structural Genomics Consortium (NESG) Target OR151
4KYV - Crystal Structure of dehalogenase HaloTag2 with HALTS at the resolution 1.8A. Northeast Structural Genomics Consortium (NESG) Target OR150
4WCV - Haloalkane dehalogenase DhaA mutant from Rhodococcus rhodochrous (T148L+G171Q+A172V+C176G)
5FLK - Structure of haloalkane dehalogenase variant DhaA101
5UXZ - X-ray crystal structure of Halotag (Haloalkane dehalogenase) bound to the P9 benzothiadiazole fluorogenic ligand
5UY1 - X-ray crystal structure of apo Halotag (Haloalkane dehalogenase)
5VNP - X-ray crystal structure of Halotag bound to the P1 benzoxadiazole fluorogenic ligand
5Y2X - Crystal structure of apo-HaloTag (M175C)
5Y2Y - Crystal structure of HaloTag (M175C) complexed with dansyl-PEG2-HaloTag ligand
6SP5 - Structure of hyperstable haloalkane dehalogenase variant DhaA115
6SP8 - Structure of hyperstable haloalkane dehalogenase variant DhaA115 prepared by the 'soak-and-freeze' method under 150 bar of krypton pressure
6TY7 - Crystal structure of haloalkane dehalogenase variant DhaA115 (synthetic construct) domain-swapped dimer type-1 (combined mutations of DhaA101 DhaA112 E20S\/F80R\/C128F\/T148L\/A155P\/A172I\/C176F\/D198W\/V219W\/C262L\/D266F)
6U2M - Crystal structure of a HaloTag-based calcium indicator, HaloCaMP V2, bound to JF635
6U32 - Crystal structure of HaloTag bound to tetramethylrhodamine-HaloTag ligand
6XT8 - Crystal structure of haloalkane dehalogenase variant DhaA115 (synthetic construct) domain-swapped dimer type-2 (combined mutations of DhaA101 DhaA112 E20S\/F80R\/C128F\/T148L\/A155P\/A172I\/C176F\/D198W\/V219W\/C262L\/D266F)
6XTC - Crystal structure of haloalkane dehalogenase variant DhaA177 domain-swapped dimer type-3
6Y7A - X-Ray structure of the Haloalkane dehalogenase HaloTag7 labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate
6Y7B - X-Ray structure of the Haloalkane dehalogenase HaloTag7 labeled with a chloroalkane-carbopyronine fluorophore substrate
6ZCC - X-Ray structure of the Haloalkane dehalogenase HOB (HaloTag7-based Oligonucleotide Binder) labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate
6ZVU - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-P174L labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate
6ZVV - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-P174W labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate
6ZVW - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate
6ZVX - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H-P174L labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate
6ZVY - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H-P174R labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate
7O3O - Structure of haloalkane dehalogenase mutant DhaA80(T148L, G171Q, A172V, C176F) from Rhodococcus rhodochrous with ionic liquid
7O8B - Structure of haloalkane dehalogenase variant DhaA80 from Rhodococcus rhodochrous
7OIP - WITHDRAWN HaloTag Engineering for Enhanced Fluorogenicity with a Simple Channel Dye 1
7OIR - WITHDRAWN HaloTag Engineering for Enhanced Fluorogenicity with a Simple Channel Dye 2
7OND - HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridine Dye
7OO4 - HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridine Dye
7PCW - X-ray structure of Haloalkane Halotag7-M175W labeled with chloralkane-tetramethylrhodamine fluorophore substrate
7PCX - X-ray structure of Haloalkane Halotag7-Q165W labeled with chloralkane-tetramethylrhodamine fluorophore substrate
7WAM - Crystal structure of HaloTag complexed with VL1
7WAN - Crystal structure of HaloTag complexed with UL2
7ZBA - Crystal structure of HaloTag complexed with Me-TRaQ-G ligand
7ZBB - Crystal structure of HaloTag complexed with TRaQ-G-ctrl ligand
7ZBD - Crystal structure of HaloTag complexed with TRaQ-G ligand
7ZIV - X-ray structure of the haloalkane dehalogenase dead variant HaloTag7-D106A bound to a chloroalkane tetramethylrhodamine fluorophore ligand (CA-TMR)
7ZIW - X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a butyltrifluoromethanesulfonamide tetramethylrhodamine ligand (TMR-T4)
7ZIX - X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a butylmethanesulfonamide tetramethylrhodamine ligand (TMR-S4)
7ZIY - X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a pentyltrifluoromethanesulfonamide tetramethylrhodamine ligand (TMR-T5)
7ZIZ - X-ray structure of the dead variant haloalkane dehalogenase HaloTag7-D106A bound to a pentanol tetramethylrhodamine ligand (TMR-Hy5)
7ZJ0 - X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a pentylmethanesulfonamide tetramethylrhodamine ligand (TMR-S5)
8B6N - X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 141-156 (cpHaloTagDelta)
8B6O - X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 141-156 (cpHaloTagDelta) fused to M13
8B6P - X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 154-156 (cpHaloTag7_154-156)
8B6Q - X-ray structure of the haloalkane dehalogenase HaloTag7 with an insertion of Calmodulin-M13 fusion at position 154-156 that mimic the structure of CaProLa, a calcium gated protein labeling technology
8B6R - X-ray structure of the haloalkane dehalogenase HaloTag7 labeled with a chloroalkane Cyanine3 fluorophore substrate
8B6S - X-ray structure of the haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG1) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate
8B6T - X-ray structure of the interface optimized haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG5-TMR) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate
8J1O - Crystal structure of HaloTag complexed with BTTA
8OE2 - Structure of hyperstable haloalkane dehalogenase variant DhaA223
8OE6 - Structure of hyperstable haloalkane dehalogenase variant DhaA231
8SW8 - Crystal Structure of HaloTag7 bound to JF669-HaloTag ligand
9JHA - X-ray structure of the Haloalkane dehalogenase HaloTag7 labeled with BD626-HTL substrate
No kinetic
No disease
(Bromomethyl)cylcohexane
1,2,3-Tribromopropane
1,2,3-trichloropropane
1,2-Dibromo-3-Chloropropane
1,2-dibromoethane
1,2-dibromopropane
1,2-dichloroethane
1,3-dibromopropane
1,3-dichloropropane
1,3-diiodopropane
1,5-dichloropentane
1-Bromo-2-chloroethane
1-Bromo-3-Chloropropane
1-Bromobutane
1-Chloro-2-(2-chloroethoxy)ethane
1-Chlorobutane
1-Chlorohexane
1-Iodobutane
1-Iodohexane
1-Iodopropane
1-bromohexane
2,3-Dichloroprop-1-ene
2-Bromo-1-Chloropropane
2-Iodobutane
3-Chloro-2-methylprop-1-ene
4-Bromobutanenitrile
Bromocyclohexane
Chlorocyclohexane
Chlorocyclopentane
BTTA
Benzoic-acid
Chloroalkane-Cyanine3-fluorophore
Chloroalkane-carbopyronine-fluorophore
Chloroalkane-tetramethylrhodamine-fluorophore
HALSTS-1Q9
HALSTS1
HyT13
JF635
JF635i
JF669-HaloTag
Me-TRaQ-G
P1-benzoxadiazole-fluorogenic-ligand
P9-benzothiadiazole
TMR-Hy5
TMR-S4
TMR-S5
TMR-T4
TMR-T5
TRaQ-G-ILQ
TRaQ-G-ILU
TRaQ-G-ctrl
UL2-8MS
VF2-Styrylpyridine-Dye
VL1-8MH
dansyl-PEG2-HaloTag
tetramethylrhodamine-HaloTag-ligand
Sequence
Peptide
MSEIGTGFPF DPHYVEVLGE RMHYVDVGPR DGTPVLFLHG NPTSSYLWRN IIPHVAPSHR CIAPDLIGMG KSDKPDLDYF FDDHVRYLDA FIEALGLEEV VLVIHDWGSA LGFHWAKRNP ERVKGIACME FIRPIPTWDE WPEFARETFQ AFRTADVGRE LIIDQNAFIE GALPKCVVRP LTEVEMDHYR EPFLKPVDRE PLWRFPNELP IAGEPANIVA LVEAYMNWLH QSPVPKLLFW GTPGVLIPPA EAARLAESLP NCKTVDIGPG LHYLQEDNPD LIGSEIARWL PAL
References (36)
| Title : Water will Find Its Way: Transport through Narrow Tunnels in Hydrolases - Sequeiros-Borja_2024_J.Chem.Inf.Model__ |
| Author(s) : Sequeiros-Borja C , Surpeta B , Thirunavukarasu AS , Dongmo Foumthuim CJ , Marchlewski I , Brezovsky J |
| Ref : J Chem Inf Model , : , 2024 |
| Abstract : |
| PubMedSearch : Sequeiros-Borja_2024_J.Chem.Inf.Model__ |
| PubMedID: 38669675 |
| Gene_locus related to this paper: canru-1lipa , rhoso-halo1 , soltu-3epoxy , human-EPHX2 |
| Title : Advancing Enzyme's Stability and Catalytic Efficiency through Synergy of Force-Field Calculations, Evolutionary Analysis, and Machine Learning - Kunka_2023_ACS.Catal_13_12506 |
| Author(s) : Kunka A , Marques SM , Havlasek M , Vasina M , Velatova N , Cengelova L , Kovar D , Damborsky J , Marek M , Bednar D , Prokop Z |
| Ref : ACS Catal , 13 :12506 , 2023 |
| Abstract : |
| PubMedSearch : Kunka_2023_ACS.Catal_13_12506 |
| PubMedID: 37822856 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : A Locally Activatable Sensor for Robust Quantification of Organellar Glutathione - Hubner_2022_bioRxiv__ |
| Author(s) : Hubner S , Quargnali G , Thallmair S , Rivera-Fuentes P |
| Ref : Biorxiv , : , 2023 |
| Abstract : |
| PubMedSearch : Hubner_2022_bioRxiv__ |
| PubMedID: |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : A general method for the development of multicolor biosensors with large dynamic ranges - Hellweg_2023_Nat.Chem.Biol__ |
| Author(s) : Hellweg L , Edenhofer A , Barck L , Huppertz MC , Frei MS , Tarnawski M , Bergner A , Koch B , Johnsson K , Hiblot J |
| Ref : Nat Chemical Biology , : , 2023 |
| Abstract : |
| PubMedSearch : Hellweg_2023_Nat.Chem.Biol__ |
| PubMedID: 37291200 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy - Kompa_2023_J.Am.Chem.Soc_145_3075 |
| Author(s) : Kompa J , Bruins J , Glogger M , Wilhelm J , Frei MS , Tarnawski M , x , Este E , Heilemann M , Hiblot J , Johnsson K |
| Ref : Journal of the American Chemical Society , 145 :3075 , 2023 |
| Abstract : |
| PubMedSearch : Kompa_2023_J.Am.Chem.Soc_145_3075 |
| PubMedID: 36716211 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : A chemical tool for blue light-inducible proximity photo-crosslinking in live cells - Mishra_2022_Chem.Sci_13_955 |
| Author(s) : Mishra PK , Kang MG , Lee H , Kim S , Choi S , Sharma N , Park CM , Ko J , Lee C , Seo JK , Rhee HW |
| Ref : Chem Sci , 13 :955 , 2022 |
| Abstract : |
| PubMedSearch : Mishra_2022_Chem.Sci_13_955 |
| PubMedID: |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Engineered HaloTag variants for fluorescence lifetime multiplexing - Frei_2022_Nat.Methods_19_65 |
| Author(s) : Frei MS , Tarnawski M , Roberti MJ , Koch B , Hiblot J , Johnsson K |
| Ref : Nat Methods , 19 :65 , 2022 |
| Abstract : |
| PubMedSearch : Frei_2022_Nat.Methods_19_65 |
| PubMedID: 34916672 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag - Wilhelm_2021_Biochemistry_60_2560 |
| Author(s) : Wilhelm J , Kuhn S , Tarnawski M , Gotthard G , Tunnermann J , Tanzer T , Karpenko J , Mertes N , Xue L , Uhrig U , Reinstein J , Hiblot J , Johnsson K |
| Ref : Biochemistry , 60 :2560 , 2021 |
| Abstract : |
| PubMedSearch : Wilhelm_2021_Biochemistry_60_2560 |
| PubMedID: 34339177 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Stabilization of Haloalkane Dehalogenase Structure by Interfacial Interaction with Ionic Liquids - Shaposhnikova_2021_Crystals_11_1052 |
| Author(s) : Shaposhnikova A , Kuty M , Chaloupkova R , Damborsky J , Smatanova IK , Minofar B , Prudnikova T |
| Ref : Crystals , 11 :1052 , 2021 |
| Abstract : |
| PubMedSearch : Shaposhnikova_2021_Crystals_11_1052 |
| PubMedID: |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : The HaloTag as a general scaffold for far-red tunable chemigenetic indicators - Deo_2021_Nat.Chem.Biol_17_718 |
| Author(s) : Deo C , Abdelfattah AS , Bhargava HK , Berro AJ , Falco N , Farrants H , Moeyaert B , Chupanova M , Lavis LD , Schreiter ER |
| Ref : Nat Chemical Biology , 17 :718 , 2021 |
| Abstract : |
| PubMedSearch : Deo_2021_Nat.Chem.Biol_17_718 |
| PubMedID: 33795886 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye - Miro-Vinyals_2021_Chembiochem_22_3398 |
| Author(s) : Miro-Vinyals C , Stein A , Fischer S , Ward TR , Deliz Liang A |
| Ref : Chembiochem , 22 :3398 , 2021 |
| Abstract : |
| PubMedSearch : Miro-Vinyals_2021_Chembiochem_22_3398 |
| PubMedID: 34609782 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Computational Enzyme Stabilization Can Affect Folding Energy Landscapes and Lead to Catalytically Enhanced Domain-Swapped Dimers - Markova_2021_ACS.Catal_11_12864 |
| Author(s) : Markova K , Kunka A , Chmelova K , Havlasek M , Babkova P , Marques SM , Vasina M , Planas-Iglesias J , Chaloupkova R , Bednar D , Prokop Z , Damborsky J , Marek M |
| Ref : ACS Catal , 11 :12864 , 2021 |
| Abstract : |
| PubMedSearch : Markova_2021_ACS.Catal_11_12864 |
| PubMedID: |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst - Markova_2020_Chem.Sci_11_11162 |
| Author(s) : Markova K , Chmelova K , Marques SM , Carpentier P , Bednar D , Damborsky J , Marek M |
| Ref : Chem Sci , 11 :11162 , 2020 |
| Abstract : |
| PubMedSearch : Markova_2020_Chem.Sci_11_11162 |
| PubMedID: 34094357 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Accurate measurement of fast endocytic recycling kinetics in real time - Jonker_2020_J.Cell.Sci_133_ |
| Author(s) : Jonker CTH , Deo C , Zager PJ , Tkachuk AN , Weinstein AM , Rodriguez-Boulan E , Lavis LD , Schreiner R |
| Ref : Journal of Cell Science , 133 : , 2020 |
| Abstract : |
| PubMedSearch : Jonker_2020_J.Cell.Sci_133_ |
| PubMedID: 31843759 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : AgHalo: A Facile Fluorogenic Sensor to Detect Drug-Induced Proteome Stress - Liu_2017_Angew.Chem.Int.Ed.Engl_56_8672 |
| Author(s) : Liu Y , Fares M , Dunham NP , Gao Z , Miao K , Jiang X , Bollinger SS , Boal AK , Zhang X |
| Ref : Angew Chem Int Ed Engl , 56 :8672 , 2017 |
| Abstract : |
| PubMedSearch : Liu_2017_Angew.Chem.Int.Ed.Engl_56_8672 |
| PubMedID: 28557281 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Structure-guided synthesis of a protein-based fluorescent sensor for alkyl halides - Kang_2017_Chem.Commun.(Camb)_53_9226 |
| Author(s) : Kang MG , Lee H , Kim BH , Dunbayev Y , Seo JK , Lee C , Rhee HW |
| Ref : Chem Commun (Camb) , 53 :9226 , 2017 |
| Abstract : |
| PubMedSearch : Kang_2017_Chem.Commun.(Camb)_53_9226 |
| PubMedID: 28766590 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : The Cation-pi Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification - Liu_2017_Biochemistry_56_1585 |
| Author(s) : Liu Y , Miao K , Dunham NP , Liu H , Fares M , Boal AK , Li X , Zhang X |
| Ref : Biochemistry , 56 :1585 , 2017 |
| Abstract : |
| PubMedSearch : Liu_2017_Biochemistry_56_1585 |
| PubMedID: 28221782 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants - Bednar_2015_PLoS.Comput.Biol_11_e1004556 |
| Author(s) : Bednar D , Beerens K , Sebestova E , Bendl J , Khare S , Chaloupkova R , Prokop Z , Brezovsky J , Baker D , Damborsky J |
| Ref : PLoS Comput Biol , 11 :e1004556 , 2015 |
| Abstract : |
| PubMedSearch : Bednar_2015_PLoS.Comput.Biol_11_e1004556 |
| PubMedID: 26529612 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Balancing the stability-activity trade-off by fine-tuning dehalogenase access tunnels - Liskova_2015_ChemCatChem_7_648 |
| Author(s) : Liskova V , Bednar D , Prudnikova T , Rezacova P , Koudelakova T , Sebestova E , Kuta-Smatanova I , Brezovsky J , Chaloupkova R , Damborsky J |
| Ref : ChemCatChem , 7 :648 , 2015 |
| Abstract : |
| PubMedSearch : Liskova_2015_ChemCatChem_7_648 |
| PubMedID: |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31 - Lahoda_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_209 |
| Author(s) : Lahoda M , Mesters JR , Stsiapanava A , Chaloupkova R , Kuty M , Damborsky J , Kuta Smatanova I |
| Ref : Acta Crystallographica D Biol Crystallogr , 70 :209 , 2014 |
| Abstract : |
| PubMedSearch : Lahoda_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_209 |
| PubMedID: 24531456 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Dynamics and hydration explain failed functional transformation in dehalogenase design - Sykora_2014_Nat.Chem.Biol_10_428 |
| Author(s) : Sykora J , Brezovsky J , Koudelakova T , Lahoda M , Fortova A , Chernovets T , Chaloupkova R , Stepankova V , Prokop Z , Smatanova IK , Hof M , Damborsky J |
| Ref : Nat Chemical Biology , 10 :428 , 2014 |
| Abstract : |
| PubMedSearch : Sykora_2014_Nat.Chem.Biol_10_428 |
| PubMedID: 24727901 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : A bidirectional system for the dynamic small molecule control of intracellular fusion proteins - Neklesa_2013_ACS.Chem.Biol_8_2293 |
| Author(s) : Neklesa TK , Noblin DJ , Kuzin AP , Lew S , Seetharaman J , Acton TB , Kornhaber GJ , Xiao R , Montelione GT , Tong L , Crews CM |
| Ref : ACS Chemical Biology , 8 :2293 , 2013 |
| Abstract : |
| PubMedSearch : Neklesa_2013_ACS.Chem.Biol_8_2293 |
| PubMedID: 23978068 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel - Koudelakova_2013_Angew.Chem.Int.Ed.Engl_52_1959 |
| Author(s) : Koudelakova T , Chaloupkova R , Brezovsky J , Prokop Z , Sebestova E , Hesseler M , Khabiri M , Plevaka M , Kulik D , Kuta Smatanova I , Rezacova P , Ettrich R , Bornscheuer UT , Damborsky J |
| Ref : Angew Chem Int Ed Engl , 52 :1959 , 2013 |
| Abstract : |
| PubMedSearch : Koudelakova_2013_Angew.Chem.Int.Ed.Engl_52_1959 |
| PubMedID: 23303607 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents - Stepankova_2013_Chembiochem_14_890 |
| Author(s) : Stepankova V , Khabiri M , Brezovsky J , Pavelka A , Sykora J , Amaro M , Minofar B , Prokop Z , Hof M , Ettrich R , Chaloupkova R , Damborsky J |
| Ref : Chembiochem , 14 :890 , 2013 |
| Abstract : |
| PubMedSearch : Stepankova_2013_Chembiochem_14_890 |
| PubMedID: 23564727 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Substrate specificity of haloalkane dehalogenases - Koudelakova_2011_Biochem.J_435_345 |
| Author(s) : Koudelakova T , Chovancova E , Brezovsky J , Monincova M , Fortova A , Jarkovsky J , Damborsky J |
| Ref : Biochemical Journal , 435 :345 , 2011 |
| Abstract : |
| PubMedSearch : Koudelakova_2011_Biochem.J_435_345 |
| PubMedID: 21294712 |
| Gene_locus related to this paper: agrtu-DHAA , brael-e2rv62 , braja-dhaa , myctu-linb , myctu-Rv1833c , rhoba-DHLA , rhoso-halo1 , sphpi-linb , xanau-halo1 |
| Title : Crystallization and crystallographic analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA mutant DhaA31 and its complex with 1,2,3-trichloropropane - Lahoda_2011_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_67_397 |
| Author(s) : Lahoda M , Chaloupkova R , Stsiapanava A , Damborsky J , Kuta Smatanova I |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 67 :397 , 2011 |
| Abstract : |
| PubMedSearch : Lahoda_2011_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_67_397 |
| PubMedID: 21393851 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands - Stsiapanava_2011_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_67_253 |
| Author(s) : Stsiapanava A , Chaloupkova R , Fortova A , Brynda J , Weiss MS , Damborsky J , Smatanova IK |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 67 :253 , 2011 |
| Abstract : |
| PubMedSearch : Stsiapanava_2011_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_67_253 |
| PubMedID: 21301099 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels - Stsiapanava_2010_Acta.Crystallogr.D.Biol.Crystallogr_66_962 |
| Author(s) : Stsiapanava A , Dohnalek J , Gavira JA , Kuty M , Koudelakova T , Damborsky J , Kuta Smatanova I |
| Ref : Acta Crystallographica D Biol Crystallogr , 66 :962 , 2010 |
| Abstract : |
| PubMedSearch : Stsiapanava_2010_Acta.Crystallogr.D.Biol.Crystallogr_66_962 |
| PubMedID: 20823547 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations - Klvana_2009_J.Mol.Biol_392_1339 |
| Author(s) : Klvana M , Pavlova M , Koudelakova T , Chaloupkova R , Dvorak P , Prokop Z , Stsiapanava A , Kuty M , Kuta-Smatanova I , Dohnalek J , Kulhanek P , Wade RC , Damborsky J |
| Ref : Journal of Molecular Biology , 392 :1339 , 2009 |
| Abstract : |
| PubMedSearch : Klvana_2009_J.Mol.Biol_392_1339 |
| PubMedID: 19577578 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis - Stsiapanava_2008_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_64_137 |
| Author(s) : Stsiapanava A , Koudelakova T , Lapkouski M , Pavlova M , Damborsky J , Smatanova IK |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 64 :137 , 2008 |
| Abstract : |
| PubMedSearch : Stsiapanava_2008_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_64_137 |
| PubMedID: 18259069 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene - Bosma_2002_Appl.Environ.Microbiol_68_3582 |
| Author(s) : Bosma T , Damborsky J , Stucki G , Janssen DB |
| Ref : Applied Environmental Microbiology , 68 :3582 , 2002 |
| Abstract : |
| PubMedSearch : Bosma_2002_Appl.Environ.Microbiol_68_3582 |
| PubMedID: 12089046 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways - Poelarends_2000_J.Bacteriol_182_2191 |
| Author(s) : Poelarends GJ , Kulakov LA , Larkin MJ , van Hylckama Vlieg JE , Janssen DB |
| Ref : Journal of Bacteriology , 182 :2191 , 2000 |
| Abstract : |
| PubMedSearch : Poelarends_2000_J.Bacteriol_182_2191 |
| PubMedID: 10735862 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Haloalkane dehalogenases: structure of a Rhodococcus enzyme - Newman_1999_Biochemistry_38_16105 |
| Author(s) : Newman J , Peat TS , Richard R , Kan L , Swanson PE , Affholter JA , Holmes IH , Schindler JF , Unkefer CJ , Terwilliger TC |
| Ref : Biochemistry , 38 :16105 , 1999 |
| Abstract : |
| PubMedSearch : Newman_1999_Biochemistry_38_16105 |
| PubMedID: 10587433 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Degradation of 1,3-dichloropropene by pseudomonas cichorii 170 - Poelarends_1998_Appl.Environ.Microbiol_64_2931 |
| Author(s) : Poelarends GJ , Wilkens M , Larkin MJ , van Elsas JD , Janssen DB |
| Ref : Applied Environmental Microbiology , 64 :2931 , 1998 |
| Abstract : |
| PubMedSearch : Poelarends_1998_Appl.Environ.Microbiol_64_2931 |
| PubMedID: 9687453 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064 - Kulakova_1997_Microbiology_143 ( Pt 1)_109 |
| Author(s) : Kulakova AN , Larkin MJ , Kulakov LA |
| Ref : Microbiology , 143 ( Pt 1) :109 , 1997 |
| Abstract : |
| PubMedSearch : Kulakova_1997_Microbiology_143 ( Pt 1)_109 |
| PubMedID: 9025284 |
| Gene_locus related to this paper: rhoso-halo1 |
| Title : Haloalkane degradation and assimilation by Rhodococcus rhodochrous NCIMB 13064 - Curragh_1994_Microbiology_140 ( Pt 6)_1433 |
| Author(s) : Curragh H , Flynn O , Larkin MJ , Stafford TM , Hamilton JT , Harper DB |
| Ref : Microbiology , 140 ( Pt 6) :1433 , 1994 |
| Abstract : |
| PubMedSearch : Curragh_1994_Microbiology_140 ( Pt 6)_1433 |
| PubMedID: 8081504 |
| Gene_locus related to this paper: rhoso-halo1 |