rhoso-halo1

Rhodococcus rhodochrous (Pseudomonas pavonaceae), Rhodococcus sp., Mycobacterium sp haloalkane dehalogenase (EC 3.8.1.5) DhaA HaloTag (hyperstable variant DhaA115)

Comment

Other strains: Rhodococcus rhodochrous (NCIMB 13064)\; Rhodococcus sp. (strain TDTM0003)\; Mycobacterium sp (strain GP1)\; Pseudomonas pavonaceae) encoded on plasmid. The HaloTag is Rhodococcus rhodochrous haloalkane dehalogenase, which has a genetically modified active site, which specifically binds the reactive chloroalkane linker and has an increased rate of ligand binding. HaloTagged fusion proteins can be expressed easily and allow enzymatic assays, cellular imaging, protein arrays, determination of sub-cellular localization.(HaloTag L47V, S58T, D78G, Y87F, L88M, C128F, A155T, E160K, A167V, A172T, K175M, C176G, K195N, A224E, N227D, E257K, T264A, H272N, Y273L) Some of the most recent structures of proteins with halotag are not included in ESTHER as the tag is only a tool and not analyzed as an alpha\/beta hydolase: see for example 7KTS 7KTR. DhaA115 thermostable multiple-point mutants || haloalkane dehalogenase Pseudomonas pavonaceae differs only with two aa of Rhodococcus haloalkane dehalogenase. Also identical to Mycobacterium sp (same paper)

Relationship

Family : Haloalkane_dehalogenase-HLD2

Block : X

Position in NCBI Life Tree : Rhodococcus rhodochrous

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Actinobacteria [phylum] N E > Actinobacteria [class] N E > Corynebacteriales N E > Nocardiaceae N E > Rhodococcus N E > Rhodococcus rhodochrous N E

Molecular evidence

No mutation

1BN6 - Rhodococcus haloalkane dehalogenase 1BN7 - Rhodococcus haloalkane dehalogenase in complex with acetate 1CQW - Rhodococcus Sp. Haloalcane dehalogenase cocrystal Nai 8J1O - Crystal structure of HaloTag complexed with BTTA 8B6N - X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 141-156 (cpHaloTagDelta) 8B6O - X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 141-156 (cpHaloTagDelta) fused to M13 8B6P - X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 154-156 (cpHaloTag7_154-156) 8B6Q - X-ray structure of the haloalkane dehalogenase HaloTag7 with an insertion of Calmodulin-M13 fusion at position 154-156 that mimic the structure of CaProLa, an calcium gated protein labeling technology 8OE6 - Structure of hyperstable haloalkane dehalogenase variant DhaA231 8OE2 - Structure of hyperstable haloalkane dehalogenase variant DhaA223 7O8B - Structure of haloalkane dehalogenase variant DhaA80 from Rhodococcus rhodochrous 7O3O - Structure of haloalkane dehalogenase mutant DhaA80(T148L, G171Q, A172V, C176F) from Rhodococcus rhodochrous with ionic liquid 8B6R - X-ray structure of the haloalkane dehalogenase HaloTag7 labeled with a chloroalkane Cyanine3 fluorophore substrate 8B6S - X-ray structure of the haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG1) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate 8B6T - X-ray structure of the interface optimized haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG5-TMR) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate 7ZIV - X-ray structure of the haloalkane dehalogenase dead variant HaloTag7-D106A bound to a chloroalkane tetramethylrhodamine fluorophore ligand (CA-TMR) 7ZIW - X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a butyltrifluoromethanesulfonamide tetramethylrhodamine ligand (TMR-T4) 7ZIX - X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a butylmethanesulfonamide tetramethylrhodamine ligand (TMR-S4) 7ZBD - Crystal structure of HaloTag complexed with TRaQ-G ligand 7ZBB - Crystal structure of HaloTag complexed with TRaQ-G-ctrl ligand 7ZBA - Crystal structure of HaloTag complexed with Me-TRaQ-G ligand 7WAM - Crystal structure of HaloTag complexed with VL1 7WAN - Crystal structure of HaloTag complexed with UL2 7ZJ0 - X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a pentylmethanesulfonamide tetramethylrhodamine ligand (TMR-S5) 7ZIZ - X-ray structure of the dead variant haloalkane dehalogenase HaloTag7-D106A bound to a pentanol tetramethylrhodamine ligand (TMR-Hy5) 7ZIY - X-ray structure of the haloalkane dehalogenase HaloTag7 bound to a pentyltrifluoromethanesulfonamide tetramethylrhodamine ligand (TMR-T5) 7PCW - X-ray structure of Haloalkane Halotag7-M175W labeled with chloralkane-tetramethylrhodamine fluorophore substrate 7PCX - X-ray structure of Haloalkane Halotag7-Q165W labeled with chloralkane-tetramethylrhodamine fluorophore substrate 5VNP - X-ray crystal structure of Halotag bound to the P1 benzoxadiazole fluorogenic ligand 7OO4 - HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridine Dye 7OND - HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridine Dye 7OIP - WITHDRAWN HaloTag Engineering for Enhanced Fluorogenicity with a Simple Channel Dye 1 7OIR - WITHDRAWN HaloTag Engineering for Enhanced Fluorogenicity with a Simple Channel Dye 2 6ZVU - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-P174L labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate 6ZVV - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-P174W labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate 6ZVW - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate 6ZVX - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H-P174L labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate 6ZVY - X-Ray structure of the Haloalkane dehalogenase HOB HaloTag7-Q165H-P174R labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate 6ZCC - X-Ray structure of the Haloalkane dehalogenase HOB (HaloTag7-based Oligonucleotide Binder) labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate 6Y7A - X-Ray structure of the Haloalkane dehalogenase HaloTag7 labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate 6Y7B - X-Ray structure of the Haloalkane dehalogenase HaloTag7 labeled with a chloroalkane-carbopyronine fluorophore substrate 6TY7 - Crystal structure of haloalkane dehalogenase variant DhaA115 (synthetic construct) domain-swapped dimer type-1 (combined mutations of DhaA101 DhaA112 E20S\/F80R\/C128F\/T148L\/A155P\/A172I\/C176F\/D198W\/V219W\/C262L\/D266F) 6XT8 - Crystal structure of haloalkane dehalogenase variant DhaA115 (synthetic construct) domain-swapped dimer type-2 (combined mutations of DhaA101 DhaA112 E20S\/F80R\/C128F\/T148L\/A155P\/A172I\/C176F\/D198W\/V219W\/C262L\/D266F) 6XTC - Crystal structure of haloalkane dehalogenase variant DhaA177 domain-swapped dimer type-3 6SP8 - Structure of hyperstable haloalkane dehalogenase variant DhaA115 prepared by the 'soak-and-freeze' method under 150 bar of krypton pressure 6SP5 - Structure of hyperstable haloalkane dehalogenase variant DhaA115 6U2M - Crystal structure of a HaloTag-based calcium indicator, HaloCaMP V2, bound to JF635 6U32 - Crystal structure of HaloTag bound to tetramethylrhodamine-HaloTag ligand 5Y2Y - Crystal structure of HaloTag (M175C) complexed with dansyl-PEG2-HaloTag ligand 5Y2X - Crystal structure of apo-HaloTag (M175C) 5UY1 - X-ray crystal structure of apo Halotag (Haloalkane dehalogenase) 5UXZ - X-ray crystal structure of Halotag (Haloalkane dehalogenase) bound to the P9 benzothiadiazole fluorogenic ligand 4KAJ - X-Ray Structure of the complex of Haloalkane dehalogenase HaloTag7 with HALTS, Northeast Structural Genomics Consortium (NESG) Target OR151 4KAA - Crystal structure of the halotag2 protein at the resolution 2.3A, Northeast Structural Genomics Consortium (NESG) target OR150 4KAC - X-Ray Structure of the complex HaloTag2 with HALTS. Northeast Structural Genomics Consortium (NESG) Target OR150. 4KAF - Crystal Structure of Haloalkane dehalogenase HaloTag7 at the resolution 1.5A, Northeast Structural Genomics Consortium (NESG) Target OR151 4KYV - Crystal Structure of dehalogenase HaloTag2 with HALTS at the resolution 1.8A. Northeast Structural Genomics Consortium (NESG) Target OR150 4WCV - Haloalkane dehalogenase DhaA mutant from Rhodococcus rhodochrous (T148L+G171Q+A172V+C176G) 5FLK - Structure of haloalkane dehalogenase variant DhaA101 4FWB - Structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant DhaA31 in complex with 1, 2, 3 - trichloropropane SUPERSEDES 3RLT 4F5Z - Crystal structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant (L95V, A172V) 4F60 - Crystal structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant (T148L, G171Q, A172V, C176F) 4E46 - Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA in complex with 2-propanol 3SK0 - Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA mutant DhaA12 3RK4 - Structure of Rhodococcus rhodochrous haloalkane dehalogenase mutant DhaA31 3FWH - Structure of haloalkane dehalogenase mutant Dha15 (I135F\/C176Y) from Rhodococcus rhodochrous 3G9X - Structure of haloalkane dehalogenase DhaA14 mutant I135F from Rhodococcus rhodochrous 4HZG - Structure of haloalkane dehalogenase DhaA from Rhodococcus rhodochrous 3FBW - Structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA mutant C176Y 2V9Z - Structure of the Rhodococcus haloalkane dehalogenase mutant with enhanced enantioselectivity

No kinetic

No disease

Sequence

Peptide

MSEIGTGFPF DPHYVEVLGE RMHYVDVGPR DGTPVLFLHG NPTSSYLWRN IIPHVAPSHR CIAPDLIGMG KSDKPDLDYF FDDHVRYLDA FIEALGLEEV VLVIHDWGSA LGFHWAKRNP ERVKGIACME FIRPIPTWDE WPEFARETFQ AFRTADVGRE LIIDQNAFIE GALPKCVVRP LTEVEMDHYR EPFLKPVDRE PLWRFPNELP IAGEPANIVA LVEAYMNWLH QSPVPKLLFW GTPGVLIPPA EAARLAESLP NCKTVDIGPG LHYLQEDNPD LIGSEIARWL PAL

References (36)

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PubMedID: 38669675
Gene_locus related to this paper: canru-1lipa , rhoso-halo1 , soltu-3epoxy , human-EPHX2

Title : A general method for the development of multicolor biosensors with large dynamic ranges - Hellweg_2023_Nat.Chem.Biol__
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Gene_locus related to this paper: rhoso-halo1

Title : Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy - Kompa_2023_J.Am.Chem.Soc_145_3075
Author(s) : Kompa J , Bruins J , Glogger M , Wilhelm J , Frei MS , Tarnawski M , x , Este E , Heilemann M , Hiblot J , Johnsson K
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Gene_locus related to this paper: rhoso-halo1

Title : Advancing Enzyme's Stability and Catalytic Efficiency through Synergy of Force-Field Calculations, Evolutionary Analysis, and Machine Learning - Kunka_2023_ACS.Catal_13_12506
Author(s) : Kunka A , Marques SM , Havlasek M , Vasina M , Velatova N , Cengelova L , Kovar D , Damborsky J , Marek M , Bednar D , Prokop Z
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Gene_locus related to this paper: rhoso-halo1

Title : A Locally Activatable Sensor for Robust Quantification of Organellar Glutathione - Hubner_2022_bioRxiv__
Author(s) : Hubner S , Quargnali G , Thallmair S , Rivera-Fuentes P
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Gene_locus related to this paper: rhoso-halo1

Title : A chemical tool for blue light-inducible proximity photo-crosslinking in live cells - Mishra_2022_Chem.Sci_13_955
Author(s) : Mishra PK , Kang MG , Lee H , Kim S , Choi S , Sharma N , Park CM , Ko J , Lee C , Seo JK , Rhee HW
Ref : Chem Sci , 13 :955 , 2022
PubMedID:
Gene_locus related to this paper: rhoso-halo1

Title : Engineered HaloTag variants for fluorescence lifetime multiplexing - Frei_2022_Nat.Methods_19_65
Author(s) : Frei MS , Tarnawski M , Roberti MJ , Koch B , Hiblot J , Johnsson K
Ref : Nat Methods , 19 :65 , 2022
PubMedID: 34916672
Gene_locus related to this paper: rhoso-halo1

Title : HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye - Miro-Vinyals_2021_Chembiochem_22_3398
Author(s) : Miro-Vinyals C , Stein A , Fischer S , Ward TR , Deliz Liang A
Ref : Chembiochem , 22 :3398 , 2021
PubMedID: 34609782
Gene_locus related to this paper: rhoso-halo1

Title : Computational Enzyme Stabilization Can Affect Folding Energy Landscapes and Lead to Catalytically Enhanced Domain-Swapped Dimers - Markova_2021_ACS.Catal_11_12864
Author(s) : Markova K , Kunka A , Chmelova K , Havlasek M , Babkova P , Marques SM , Vasina M , Planas-Iglesias J , Chaloupkova R , Bednar D , Prokop Z , Damborsky J , Marek M
Ref : ACS Catal , 11 :12864 , 2021
PubMedID:
Gene_locus related to this paper: rhoso-halo1

Title : Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag - Wilhelm_2021_Biochemistry_60_2560
Author(s) : Wilhelm J , Kuhn S , Tarnawski M , Gotthard G , Tunnermann J , Tanzer T , Karpenko J , Mertes N , Xue L , Uhrig U , Reinstein J , Hiblot J , Johnsson K
Ref : Biochemistry , 60 :2560 , 2021
PubMedID: 34339177
Gene_locus related to this paper: rhoso-halo1

Title : Stabilization of Haloalkane Dehalogenase Structure by Interfacial Interaction with Ionic Liquids - Shaposhnikova_2021_Crystals_11_1052
Author(s) : Shaposhnikova A , Kuty M , Chaloupkova R , Damborsky J , Smatanova IK , Minofar B , Prudnikova T
Ref : Crystals , 11 :1052 , 2021
PubMedID:
Gene_locus related to this paper: rhoso-halo1

Title : The HaloTag as a general scaffold for far-red tunable chemigenetic indicators - Deo_2021_Nat.Chem.Biol_17_718
Author(s) : Deo C , Abdelfattah AS , Bhargava HK , Berro AJ , Falco N , Farrants H , Moeyaert B , Chupanova M , Lavis LD , Schreiter ER
Ref : Nat Chemical Biology , 17 :718 , 2021
PubMedID: 33795886
Gene_locus related to this paper: rhoso-halo1

Title : Accurate measurement of fast endocytic recycling kinetics in real time - Jonker_2020_J.Cell.Sci_133_
Author(s) : Jonker CTH , Deo C , Zager PJ , Tkachuk AN , Weinstein AM , Rodriguez-Boulan E , Lavis LD , Schreiner R
Ref : Journal of Cell Science , 133 : , 2020
PubMedID: 31843759
Gene_locus related to this paper: rhoso-halo1

Title : Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst - Markova_2020_Chem.Sci_11_11162
Author(s) : Markova K , Chmelova K , Marques SM , Carpentier P , Bednar D , Damborsky J , Marek M
Ref : Chem Sci , 11 :11162 , 2020
PubMedID: 34094357
Gene_locus related to this paper: rhoso-halo1

Title : AgHalo: A Facile Fluorogenic Sensor to Detect Drug-Induced Proteome Stress - Liu_2017_Angew.Chem.Int.Ed.Engl_56_8672
Author(s) : Liu Y , Fares M , Dunham NP , Gao Z , Miao K , Jiang X , Bollinger SS , Boal AK , Zhang X
Ref : Angew Chem Int Ed Engl , 56 :8672 , 2017
PubMedID: 28557281
Gene_locus related to this paper: rhoso-halo1

Title : Structure-guided synthesis of a protein-based fluorescent sensor for alkyl halides - Kang_2017_Chem.Commun.(Camb)_53_9226
Author(s) : Kang MG , Lee H , Kim BH , Dunbayev Y , Seo JK , Lee C , Rhee HW
Ref : Chem Commun (Camb) , 53 :9226 , 2017
PubMedID: 28766590
Gene_locus related to this paper: rhoso-halo1

Title : The Cation-pi Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification - Liu_2017_Biochemistry_56_1585
Author(s) : Liu Y , Miao K , Dunham NP , Liu H , Fares M , Boal AK , Li X , Zhang X
Ref : Biochemistry , 56 :1585 , 2017
PubMedID: 28221782
Gene_locus related to this paper: rhoso-halo1

Title : FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants - Bednar_2015_PLoS.Comput.Biol_11_e1004556
Author(s) : Bednar D , Beerens K , Sebestova E , Bendl J , Khare S , Chaloupkova R , Prokop Z , Brezovsky J , Baker D , Damborsky J
Ref : PLoS Comput Biol , 11 :e1004556 , 2015
PubMedID: 26529612
Gene_locus related to this paper: rhoso-halo1

Title : Balancing the stability-activity trade-off by fine-tuning dehalogenase access tunnels - Liskova_2015_ChemCatChem_7_648
Author(s) : Liskova V , Bednar D , Prudnikova T , Rezacova P , Koudelakova T , Sebestova E , Kuta-Smatanova I , Brezovsky J , Chaloupkova R , Damborsky J
Ref : ChemCatChem , 7 :648 , 2015
PubMedID:
Gene_locus related to this paper: rhoso-halo1

Title : Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31 - Lahoda_2014_Acta.Crystallogr.D.Biol.Crystallogr_70_209
Author(s) : Lahoda M , Mesters JR , Stsiapanava A , Chaloupkova R , Kuty M , Damborsky J , Kuta Smatanova I
Ref : Acta Crystallographica D Biol Crystallogr , 70 :209 , 2014
PubMedID: 24531456
Gene_locus related to this paper: rhoso-halo1

Title : Dynamics and hydration explain failed functional transformation in dehalogenase design - Sykora_2014_Nat.Chem.Biol_10_428
Author(s) : Sykora J , Brezovsky J , Koudelakova T , Lahoda M , Fortova A , Chernovets T , Chaloupkova R , Stepankova V , Prokop Z , Smatanova IK , Hof M , Damborsky J
Ref : Nat Chemical Biology , 10 :428 , 2014
PubMedID: 24727901
Gene_locus related to this paper: rhoso-halo1

Title : A bidirectional system for the dynamic small molecule control of intracellular fusion proteins - Neklesa_2013_ACS.Chem.Biol_8_2293
Author(s) : Neklesa TK , Noblin DJ , Kuzin AP , Lew S , Seetharaman J , Acton TB , Kornhaber GJ , Xiao R , Montelione GT , Tong L , Crews CM
Ref : ACS Chemical Biology , 8 :2293 , 2013
PubMedID: 23978068
Gene_locus related to this paper: rhoso-halo1

Title : Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel - Koudelakova_2013_Angew.Chem.Int.Ed.Engl_52_1959
Author(s) : Koudelakova T , Chaloupkova R , Brezovsky J , Prokop Z , Sebestova E , Hesseler M , Khabiri M , Plevaka M , Kulik D , Kuta Smatanova I , Rezacova P , Ettrich R , Bornscheuer UT , Damborsky J
Ref : Angew Chem Int Ed Engl , 52 :1959 , 2013
PubMedID: 23303607
Gene_locus related to this paper: rhoso-halo1

Title : Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents - Stepankova_2013_Chembiochem_14_890
Author(s) : Stepankova V , Khabiri M , Brezovsky J , Pavelka A , Sykora J , Amaro M , Minofar B , Prokop Z , Hof M , Ettrich R , Chaloupkova R , Damborsky J
Ref : Chembiochem , 14 :890 , 2013
PubMedID: 23564727
Gene_locus related to this paper: rhoso-halo1

Title : Crystallization and crystallographic analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA mutant DhaA31 and its complex with 1,2,3-trichloropropane - Lahoda_2011_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_67_397
Author(s) : Lahoda M , Chaloupkova R , Stsiapanava A , Damborsky J , Kuta Smatanova I
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 67 :397 , 2011
PubMedID: 21393851
Gene_locus related to this paper: rhoso-halo1

Title : Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands - Stsiapanava_2011_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_67_253
Author(s) : Stsiapanava A , Chaloupkova R , Fortova A , Brynda J , Weiss MS , Damborsky J , Smatanova IK
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 67 :253 , 2011
PubMedID: 21301099
Gene_locus related to this paper: rhoso-halo1

Title : Substrate specificity of haloalkane dehalogenases - Koudelakova_2011_Biochem.J_435_345
Author(s) : Koudelakova T , Chovancova E , Brezovsky J , Monincova M , Fortova A , Jarkovsky J , Damborsky J
Ref : Biochemical Journal , 435 :345 , 2011
PubMedID: 21294712
Gene_locus related to this paper: agrtu-DHAA , brael-e2rv62 , braja-dhaa , myctu-linb , myctu-Rv1833c , rhoba-DHLA , rhoso-halo1 , sphpi-linb , xanau-halo1

Title : Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels - Stsiapanava_2010_Acta.Crystallogr.D.Biol.Crystallogr_66_962
Author(s) : Stsiapanava A , Dohnalek J , Gavira JA , Kuty M , Koudelakova T , Damborsky J , Kuta Smatanova I
Ref : Acta Crystallographica D Biol Crystallogr , 66 :962 , 2010
PubMedID: 20823547
Gene_locus related to this paper: rhoso-halo1

Title : Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations - Klvana_2009_J.Mol.Biol_392_1339
Author(s) : Klvana M , Pavlova M , Koudelakova T , Chaloupkova R , Dvorak P , Prokop Z , Stsiapanava A , Kuty M , Kuta-Smatanova I , Dohnalek J , Kulhanek P , Wade RC , Damborsky J
Ref : Journal of Molecular Biology , 392 :1339 , 2009
PubMedID: 19577578
Gene_locus related to this paper: rhoso-halo1

Title : Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis - Stsiapanava_2008_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_64_137
Author(s) : Stsiapanava A , Koudelakova T , Lapkouski M , Pavlova M , Damborsky J , Smatanova IK
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 64 :137 , 2008
PubMedID: 18259069
Gene_locus related to this paper: rhoso-halo1

Title : Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene - Bosma_2002_Appl.Environ.Microbiol_68_3582
Author(s) : Bosma T , Damborsky J , Stucki G , Janssen DB
Ref : Applied Environmental Microbiology , 68 :3582 , 2002
PubMedID: 12089046
Gene_locus related to this paper: rhoso-halo1

Title : Roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways - Poelarends_2000_J.Bacteriol_182_2191
Author(s) : Poelarends GJ , Kulakov LA , Larkin MJ , van Hylckama Vlieg JE , Janssen DB
Ref : Journal of Bacteriology , 182 :2191 , 2000
PubMedID: 10735862
Gene_locus related to this paper: rhoso-halo1

Title : Haloalkane dehalogenases: structure of a Rhodococcus enzyme - Newman_1999_Biochemistry_38_16105
Author(s) : Newman J , Peat TS , Richard R , Kan L , Swanson PE , Affholter JA , Holmes IH , Schindler JF , Unkefer CJ , Terwilliger TC
Ref : Biochemistry , 38 :16105 , 1999
PubMedID: 10587433
Gene_locus related to this paper: rhoso-halo1

Title : Degradation of 1,3-dichloropropene by pseudomonas cichorii 170 - Poelarends_1998_Appl.Environ.Microbiol_64_2931
Author(s) : Poelarends GJ , Wilkens M , Larkin MJ , van Elsas JD , Janssen DB
Ref : Applied Environmental Microbiology , 64 :2931 , 1998
PubMedID: 9687453
Gene_locus related to this paper: rhoso-halo1

Title : The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064 - Kulakova_1997_Microbiology_143 ( Pt 1)_109
Author(s) : Kulakova AN , Larkin MJ , Kulakov LA
Ref : Microbiology , 143 ( Pt 1) :109 , 1997
PubMedID: 9025284
Gene_locus related to this paper: rhoso-halo1

Title : Haloalkane degradation and assimilation by Rhodococcus rhodochrous NCIMB 13064 - Curragh_1994_Microbiology_140 ( Pt 6)_1433
Author(s) : Curragh H , Flynn O , Larkin MJ , Stafford TM , Hamilton JT , Harper DB
Ref : Microbiology , 140 ( Pt 6) :1433 , 1994
PubMedID: 8081504
Gene_locus related to this paper: rhoso-halo1