Iwasaki T

References (3)

Title : Serum butyrylcholinesterase is strongly associated with adiposity, the serum lipid profile and insulin resistance - Iwasaki_2007_Intern.Med_46_1633
Author(s) : Iwasaki T , Yoneda M , Nakajima A , Terauchi Y
Ref : Intern Med , 46 :1633 , 2007
Abstract : OBJECTIVE: We investigated the association between the serum butyrylcholinesterase (BChE) activity and parameters of adiposity, insulin resistance and the serum lipid profile, including the serum levels of triglyceride (TG), HDL-cholesterol (HDL-C) and LDL-cholesterol (LDL-C) in Japanese patients with type 2 diabetes and non-diabetic subjects. PATIENTS AND
METHODS: A total of 259 Japanese subjects [171 patients with type 2 diabetes (age 60.4+/-11.9 years, BMI 25.0+/-4.4) and 88 non-diabetic subjects (age 54.2+/-15.0 years, BMI 25.8+/-4.7)] were enrolled in the study. To assess the hepatic fat content, the ratio of the CT attenuation value of the liver to that of the spleen (L/S ratio) was calculated.
RESULTS: Serum BChE activity was significantly correlated with various indices of adiposity, including the waist circumference, L/S ratio, visceral fat area (VFA), subcutaneous fat area (SFA) and BMI, and also with the TG, HDL-C, LDL-C and HOMA-R, in the entire subject population. Even after adjustment for five variables, namely, age, gender, HbA1c, CRP and serum ferritin (the serum levels of ferritin and CRP have been reported to be associated with adiposity and insulin resistance), the serum BChE activity was still correlated with the waist circumference, L/S ratio, VFA, SFA, BMI, TG, HDL-C, LDL-C and HOMA-R. Furthermore, changes in the serum BChE activity were associated with changes in the L/S ratio, VFA and SFA at 1 year after the baseline.
CONCLUSIONS: These results indicate that the serum BChE activity is associated with parameters of adiposity, the serum lipid profile and the degree of insulin resistance.
ESTHER : Iwasaki_2007_Intern.Med_46_1633
PubMedSearch : Iwasaki_2007_Intern.Med_46_1633
PubMedID: 17917325

Title : Functional impairment of two novel mutations detected in lipoprotein-associated phospholipase A2 (Lp-PLA2) deficiency patients - Ishihara_2004_J.Hum.Genet_49_302
Author(s) : Ishihara M , Iwasaki T , Nagano M , Ishii J , Takano M , Kujiraoka T , Tsuji M , Hattori H , Emi M
Ref : J Hum Genet , 49 :302 , 2004
Abstract : Plasma lipoprotein-associated phospholipase A2 (Lp-PLA2), also known as platelet-activating factor (PAF) acetylhydrolase (PAF-AH), is a member of the serine-dependent class of A2 phospholipases that hydrolyze sn2-ester bonds of fragmented or oxidized phospholipids at sites where atherosclerotic plaques are forming. Most circulating Lp-PLA2 is bound to low-density lipoprotein (LDL) particles in plasma and the rest to high-density lipoprotein (HDL). Deficiency of Lp-PLA2 is a predisposing factor for cardiovascular diseases in the Japanese population. We describe here two novel mutations of the gene encoding Lp-PLA2, InsA191 and I317N in Japanese subjects. The first patient, with partial Lp-PLA2 deficiency, was heterozygous for the InsA191 mutation; macrophages from this patient secreted only half the normal amount of Lp-PLA2 in vitro. The other patient, who showed complete Lp-PLA2 deficiency, was a compound heterozygote for the novel I317N mutation and a common V279F mutation; macrophages from that patient failed to secrete any Lp-PLA2. Measurement of Lp-PLA2 mass, activity and Western blotting verified impaired production and secretion of the enzyme after transfection of mutant construct into COS-7 cells. These results indicated that both novel mutants, InsA191 and I317N, impair function of the Lp-PLA2 gene.
ESTHER : Ishihara_2004_J.Hum.Genet_49_302
PubMedSearch : Ishihara_2004_J.Hum.Genet_49_302
PubMedID: 15148590
Gene_locus related to this paper: human-PLA2G7

Title : [Effects of early postnatal AF64A treatment on passive avoidance response and radial maze learning in rats] - Nonaka_1998_Nihon.Shinkei.Seishin.Yakurigaku.Zasshi_18_39
Author(s) : Nonaka H , Ichitani Y , Iwasaki T
Ref : Nihon Shinkei Seishin Yakurigaku Zasshi , 18 :39 , 1998
Abstract : In order to investigate how the selectively lesioned cholinergic system at the early postnatal age influences adult learning behavior, the effects of postnatal administration of ethylcholine mustard aziridinium ion (AF64A), a selective cholinergic neurotoxin, on the acquisition of 2 kinds of learning tasks were examined. Rat pups received an intraventricular injection of AF64A (1.0 or 2.0 nmol) or saline on postnatal day 8, and in adulthood (at 3 months of age), they were tested with the acquisition of passive avoidance response (PAR) and 8-arm radial maze learning. In PAR testing, a significant impairment was observed in male AF64A-treated rats. In addition, in the radial maze task, AF64A-treated rats needed significantly more trials to acquire the task as compared with saline-treated animals. Histological examination after behavioral testings revealed a marked reduction of acetylcholinesterase-stained fibers in the hippocampus and dentate gyrus of the AF64A-treated groups, while there were no detectable changes in the striatum or cerebral cortex. The results suggest that early postnatal AF64A administration induced learning deficits in adulthood which were associated with long-lasting cholinergic denervation in hippocampal formation.
ESTHER : Nonaka_1998_Nihon.Shinkei.Seishin.Yakurigaku.Zasshi_18_39
PubMedSearch : Nonaka_1998_Nihon.Shinkei.Seishin.Yakurigaku.Zasshi_18_39
PubMedID: 9656231