Maeno Y

References (2)

Title : Acute effects of a sarin-like organophosphorus agent, bis(isopropyl methyl)phosphonate, on cardiovascular parameters in anaesthetized, artificially ventilated rats - Watanabe_2013_Toxicol.Appl.Pharmacol_272_61
Author(s) : Watanabe Y , Itoh T , Shiraishi H , Maeno Y , Arima Y , Torikoshi A , Namera A , Makita R , Yoshizumi M , Nagao M
Ref : Toxicol Appl Pharmacol , 272 :61 , 2013
Abstract : The organophosphorus compound sarin irreversibly inhibits acetylcholinesterase. We examined the acute cardiovascular effects of a sarin-like organophosphorus agent, bis(isopropyl methyl)phosphonate (BIMP), in anaesthetized, artificially ventilated rats. Intravenous administration of BIMP (0.8mg/kg; the LD50 value) induced a long-lasting increase in blood pressure and tended to increase heart rate. In rats pretreated with the non-selective muscarinic-receptor antagonist atropine, BIMP significantly increased both heart rate and blood pressure. In atropine-treated rats, hexamethonium (antagonist of ganglionic nicotinic receptors) greatly attenuated the BIMP-induced increase in blood pressure without changing the BIMP-induced increase in heart rate. In rats treated with atropine plus hexamethonium, intravenous phentolamine (non-selective alpha-adrenergic receptor antagonist) plus propranolol (non-selective beta-adrenergic receptor antagonist) completely blocked the BIMP-induced increases in blood pressure and heart rate. In atropine-treated rats, the reversible acetylcholinesterase inhibitor neostigmine (1mg/kg) induced a transient increase in blood pressure, but had no effect on heart rate. These results suggest that in anaesthetized rats, BIMP induces powerful stimulation of sympathetic as well as parasympathetic nerves and thereby modulates heart rate and blood pressure. They may also indicate that an action independent of acetylcholinesterase inhibition contributes to the acute cardiovascular responses induced by BIMP.
ESTHER : Watanabe_2013_Toxicol.Appl.Pharmacol_272_61
PubMedSearch : Watanabe_2013_Toxicol.Appl.Pharmacol_272_61
PubMedID: 23769715

Title : Development of forensic diagnosis of acute sarin poisoning - Nagao_2003_Leg.Med.(Tokyo)_5 Suppl 1_S34
Author(s) : Nagao M , Takatori T , Maeno Y , Isobe I , Koyama H , Tsuchimochi T
Ref : Leg Med (Tokyo) , 5 Suppl 1 :S34 , 2003
Abstract : On March 20, 1995, the Tokyo subway system was subjected to a horrifying terrorist attack with sarin gas (isopropyl methylphosphonofluoridate) that left 12 persons dead and over 5000 injured. In order to diagnose the definite cause of death of the victims, a new method was developed to detect sarin hydrolysis products in the erythrocytes and formalin-fixed cerebella from four victims of sarin poisoning. Sarin-bound acetylcholinesterase (AChE) was solubilized from the specimens of sarin victims and digested with trypsin. The sarin hydrolysis products bound to AChE were released by alkaline phosphatase digestion. The digested sarin hydrolysis products were subjected to trimethylsilyl derivatization and detected by gas chromatography-mass spectrometry. Sarin hydrolysis products were detected in all sarin poisoning victims.
ESTHER : Nagao_2003_Leg.Med.(Tokyo)_5 Suppl 1_S34
PubMedSearch : Nagao_2003_Leg.Med.(Tokyo)_5 Suppl 1_S34
PubMedID: 12935549