Molenaar D

References (4)

Title : New Insights Into Cinnamoyl Esterase Activity of Oenococcus oeni - Collombel_2019_Front.Microbiol_10_2597
Author(s) : Collombel I , Melkonian C , Molenaar D , Campos FM , Hogg T
Ref : Front Microbiol , 10 :2597 , 2019
Abstract : Some strains of Oenococcus oeni possess cinnamoyl esterase activity that can be relevant in the malolactic stage of wine production liberating hydroxycinnamic acids that are precursors of volatile phenols responsible for sensory faults. The objective of this study was to better understand the basis of the differential activity between strains. After initial screening, five commercial strains of O. oeni were selected, three were found to exhibit cinnamoyl esterase activity (CE+) and two not (CE-). Although the use of functional annotation of genes revealed genotypic variations between the strains, no specific genes common only to the three CE+ strains could explain the different activities. Pasteurized wine was used as a natural source of tartrate esters in growth and metabolism experiments conducted in MRS medium, whilst commercial trans-caftaric acid was used as substrate for enzyme assays. Detoxification did not seem to be the main biological mechanism involved in the activity since unlike its phenolic cleavage products and their immediate metabolites (trans-caffeic acid and 4-ethylcatechol), trans-caftaric acid was not toxic toward O. oeni. In the case of the two CE+ strains Oenos(TM) and CiNe(TM), wine-exposed samples showed a more rapid degradation of trans-caftaric acid than the unexposed ones. The CE activity was present in all cell-free extracts of both wine-exposed and unexposed strains, except in the cell-free extracts of the CE- strain CH11(TM). This activity may be constitutive rather than induced by exposure to tartrate esters. Trans-caftaric acid was totally cleaved to trans-caffeic acid by cell-free extracts of the three CE+ strains, whilst cell-free extracts of the CE- strain CH16(TM) showed significantly lower activity, although higher for the strains in experiments with no prior wine exposure. The EstB28 esterase gene, found in the genomes of the 5 strains, did not reveal any difference on the upstream regulation and transport functionality between the strains. This study highlights the complexity of the basis of this activity in wine related O. oeni population. Variable cinnamoyl esterases or/and membrane transport activities in the O. oeni strains analyzed and a possible implication of wine molecules could explain this phenomenon.
ESTHER : Collombel_2019_Front.Microbiol_10_2597
PubMedSearch : Collombel_2019_Front.Microbiol_10_2597
PubMedID: 31781078

Title : Complete genome sequence of Lactococcus lactis subsp. lactis KF147, a plant-associated lactic acid bacterium - Siezen_2010_J.Bacteriol_192_2649
Author(s) : Siezen RJ , Bayjanov J , Renckens B , Wels M , van Hijum SA , Molenaar D , van Hylckama Vlieg JE
Ref : Journal of Bacteriology , 192 :2649 , 2010
Abstract : Lactococcus lactis is a lactic acid bacterium used in the production of many fermented dairy products. We report the complete genome sequence of L. lactis subsp. lactis KF147, a nondairy strain isolated from mung bean sprouts. The circular chromosome of 2,598,144 bp, the largest among the sequenced lactococcal strains, encodes many properties related to adaptation to the plant environment.
ESTHER : Siezen_2010_J.Bacteriol_192_2649
PubMedSearch : Siezen_2010_J.Bacteriol_192_2649
PubMedID: 20348266
Gene_locus related to this paper: lacla-cpo , lacla-menX , lacla-YQAG , laclk-a9qsc5 , laclk-d2bl62

Title : Genome-scale genotype-phenotype matching of two Lactococcus lactis isolates from plants identifies mechanisms of adaptation to the plant niche - Siezen_2008_Appl.Environ.Microbiol_74_424
Author(s) : Siezen RJ , Starrenburg MJ , Boekhorst J , Renckens B , Molenaar D , van Hylckama Vlieg JE
Ref : Applied Environmental Microbiology , 74 :424 , 2008
Abstract : Lactococcus lactis is a primary constituent of many starter cultures used for the manufacturing of fermented dairy products, but the species also occurs in various nondairy niches such as (fermented) plant material. Three genome sequences of L. lactis dairy strains (IL-1403, SK11, and MG1363) are publicly available. An extensive molecular and phenotypic diversity analysis was now performed on two L. lactis plant isolates. Diagnostic sequencing of their genomes resulted in over 2.5 Mb of sequence for each strain. A high synteny was found with the genome of L. lactis IL-1403, which was used as a template for contig mapping and locating deletions and insertions in the plant L. lactis genomes. Numerous genes were identified that do not have homologs in the published genome sequences of dairy L. lactis strains. Adaptation to growth on substrates derived from plant cell walls is evident from the presence of gene sets for the degradation of complex plant polymers such as xylan, arabinan, glucans, and fructans but also for the uptake and conversion of typical plant cell wall degradation products such as alpha-galactosides, beta-glucosides, arabinose, xylose, galacturonate, glucuronate, and gluconate. Further niche-specific differences are found in genes for defense (nisin biosynthesis), stress response (nonribosomal peptide synthesis and various transporters), and exopolysaccharide biosynthesis, as well as the expected differences in various mobile elements such as prophages, plasmids, restriction-modification systems, and insertion sequence elements. Many of these genes were identified for the first time in Lactococcus lactis. In most cases good correspondence was found with the phenotypic characteristics of these two strains.
ESTHER : Siezen_2008_Appl.Environ.Microbiol_74_424
PubMedSearch : Siezen_2008_Appl.Environ.Microbiol_74_424
PubMedID: 18039825
Gene_locus related to this paper: lacla-cpo , lacla-menX , lacla-YQAG , laclk-a9qsc5 , laclk-a9qse8 , laclk-d2bl62

Title : Complete genome sequence of Lactobacillus plantarum WCFS1 - Kleerebezem_2003_Proc.Natl.Acad.Sci.U.S.A_100_1990
Author(s) : Kleerebezem M , Boekhorst J , van Kranenburg R , Molenaar D , Kuipers OP , Leer R , Tarchini R , Peters SA , Sandbrink HM , Fiers MW , Stiekema W , Lankhorst RM , Bron PA , Hoffer SM , Groot MN , Kerkhoven R , De Vries M , Ursing B , de Vos WM , Siezen RJ
Ref : Proc Natl Acad Sci U S A , 100 :1990 , 2003
Abstract : The 3,308,274-bp sequence of the chromosome of Lactobacillus plantarum strain WCFS1, a single colony isolate of strain NCIMB8826 that was originally isolated from human saliva, has been determined, and contains 3,052 predicted protein-encoding genes. Putative biological functions could be assigned to 2,120 (70%) of the predicted proteins. Consistent with the classification of L. plantarum as a facultative heterofermentative lactic acid bacterium, the genome encodes all enzymes required for the glycolysis and phosphoketolase pathways, all of which appear to belong to the class of potentially highly expressed genes in this organism, as was evident from the codon-adaptation index of individual genes. Moreover, L. plantarum encodes a large pyruvate-dissipating potential, leading to various end-products of fermentation. L. plantarum is a species that is encountered in many different environmental niches, and this flexible and adaptive behavior is reflected by the relatively large number of regulatory and transport functions, including 25 complete PTS sugar transport systems. Moreover, the chromosome encodes >200 extracellular proteins, many of which are predicted to be bound to the cell envelope. A large proportion of the genes encoding sugar transport and utilization, as well as genes encoding extracellular functions, appear to be clustered in a 600-kb region near the origin of replication. Many of these genes display deviation of nucleotide composition, consistent with a foreign origin. These findings suggest that these genes, which provide an important part of the interaction of L. plantarum with its environment, form a lifestyle adaptation region in the chromosome.
ESTHER : Kleerebezem_2003_Proc.Natl.Acad.Sci.U.S.A_100_1990
PubMedSearch : Kleerebezem_2003_Proc.Natl.Acad.Sci.U.S.A_100_1990
PubMedID: 12566566
Gene_locus related to this paper: lacpl-EST1 , lacpl-EST2 , lacpl-HPO , lacpl-LP.0461 , lacpl-LP.0618 , lacpl-LP.0796 , lacpl-LP.0973 , lacpl-LP.1002 , lacpl-LP.1124 , lacpl-LP.1156 , lacpl-LP.1165 , lacpl-LP.1760 , lacpl-LP.1774 , lacpl-LP.1935 , lacpl-LP.2519 , lacpl-LP.2586 , lacpl-LP.2620 , lacpl-LP.2631 , lacpl-LP.2737 , lacpl-LP.2923 , lacpl-LP.2953 , lacpl-LP.3205 , lacpl-LP.3265 , lacpl-LP.3341 , lacpl-LP.3393 , lacpl-LP.3561 , lacpl-LP.3562 , lacpl-PEPI , lacpl-PEPR1 , lacpl-PEPR2 , lacpl-pepx , lacpl-tanL