de Vos WM

References (11)

Title : Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera - Sun_2015_Nat.Commun_6_8322
Author(s) : Sun Z , Harris HM , McCann A , Guo C , Argimon S , Zhang W , Yang X , Jeffery IB , Cooney JC , Kagawa TF , Liu W , Song Y , Salvetti E , Wrobel A , Rasinkangas P , Parkhill J , Rea MC , O'Sullivan O , Ritari J , Douillard FP , Paul Ross R , Yang R , Briner AE , Felis GE , de Vos WM , Barrangou R , Klaenhammer TR , Caufield PW , Cui Y , Zhang H , O'Toole PW
Ref : Nat Commun , 6 :8322 , 2015
Abstract : Lactobacilli are a diverse group of species that occupy diverse nutrient-rich niches associated with humans, animals, plants and food. They are used widely in biotechnology and food preservation, and are being explored as therapeutics. Exploiting lactobacilli has been complicated by metabolic diversity, unclear species identity and uncertain relationships between them and other commercially important lactic acid bacteria. The capacity for biotransformations catalysed by lactobacilli is an untapped biotechnology resource. Here we report the genome sequences of 213 Lactobacillus strains and associated genera, and their encoded genetic catalogue for modifying carbohydrates and proteins. In addition, we describe broad and diverse presence of novel CRISPR-Cas immune systems in lactobacilli that may be exploited for genome editing. We rationalize the phylogenomic distribution of host interaction factors and bacteriocins that affect their natural and industrial environments, and mechanisms to withstand stress during technological processes. We present a robust phylogenomic framework of existing species and for classifying new species.
ESTHER : Sun_2015_Nat.Commun_6_8322
PubMedSearch : Sun_2015_Nat.Commun_6_8322
PubMedID: 26415554
Gene_locus related to this paper: 9laco-a0a0r1hz65 , 9laco-a0a0r1j1t4 , 9laco-a0a0r1j3p0 , 9laco-a0a0r1k3i0 , 9laco-a0a0r1k563 , 9laco-a0a0r1kgb3 , 9laco-a0a0r1kji2 , 9laco-a0a0r1kwq5 , 9laco-a0a0r1l700 , 9laco-a0a0r1p6l8 , 9laco-a0a0r1q939 , 9laco-a0a0r1qv39 , 9laco-a0a0r1wj75 , laccl-a0a0r2bne3 , 9laco-a0a0r2dnk9 , 9laco-a0a0r2ds70 , 9laco-a0a0r2k127 , 9laco-a0a0r2lee7 , 9laco-a0a0r2lqt2 , 9laco-a0a0r2m354 , 9laco-a0a0r2n9f2 , 9laco-a0a0r2nrk2 , lacze-a0a0r1ekw6 , 9laco-a0a0r1ju11 , 9laco-a0a0r1k516 , 9laco-a0a0r1leq9 , 9laco-a0a0r1lul8 , 9laco-a0a0r1lzg4 , 9laco-a0a0r1mhp8 , 9laco-a0a0r1mjt1 , 9laco-a0a0r1nv21 , 9laco-a0a0r1q1p6 , 9laco-a0a0r1qm41 , 9laco-a0a0r1qs58 , 9laco-a0a0r1rgu0 , 9laco-a0a0r1tg12 , 9laco-a0a0r1u777 , 9laco-a0a0r1ufv3 , 9laco-a0a0r1ul77 , 9laco-a0a0r1vad0 , 9laco-a0a0r1w3f4 , 9laco-a0a0r1w9r8 , 9laco-a0a0r1wpq2 , 9laco-a0a0r1x2g3 , 9laco-a0a0r2abe6 , 9laco-a0a0r2b6w1 , 9laco-a0a0r2b8g1 , 9laco-a0a0r2ch10 , 9laco-a0a0r2cld6 , 9laco-a0a0r2cv38 , 9laco-a0a0r2d3x3 , 9laco-a0a0r2dct2 , 9laco-a0a0r2flt3 , 9laco-a0a0r2frk5 , 9laco-a0a0r2guq4 , 9firm-a0a0r2h5m0 , weivi-a0a0r2h8r4 , 9lact-a0a0r2hnx4 , 9lact-a0a0r2jkt6 , 9lact-a0a0r2jmz1 , 9laco-a0a0r2jwg5 , 9laco-a0a0r2jxu0 , 9laco-a0a0r2jxw0 , lacam-a0a0r2kgt3 , 9laco-a0a0r2kx86 , 9laco-a0a0r2mxi6 , 9laco-a0a0r1vln2 , 9laco-a0a0r2ca25 , 9laco-a0a0r1zjs2 , weipa-c5rbw8

Title : Genome sequence of the verrucomicrobium Opitutus terrae PB90-1, an abundant inhabitant of rice paddy soil ecosystems - van Passel_2011_J.Bacteriol_193_2367
Author(s) : van Passel MW , Kant R , Palva A , Copeland A , Lucas S , Lapidus A , Glavina Del Rio T , Pitluck S , Goltsman E , Clum A , Sun H , Schmutz J , Larimer FW , Land ML , Hauser L , Kyrpides N , Mikhailova N , Richardson PP , Janssen PH , de Vos WM , Smidt H
Ref : Journal of Bacteriology , 193 :2367 , 2011
Abstract : Bacteria of the deeply branching phylum Verrucomicrobia are rarely cultured yet commonly detected in metagenomic libraries from aquatic, terrestrial, and intestinal environments. We have sequenced the genome of Opitutus terrae PB90-1, a fermentative anaerobe within this phylum, isolated from rice paddy soil and capable of propionate production from plant-derived polysaccharides.
ESTHER : van Passel_2011_J.Bacteriol_193_2367
PubMedSearch : van Passel_2011_J.Bacteriol_193_2367
PubMedID: 21398538
Gene_locus related to this paper: opitp-b1zrm2

Title : Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor - O'Connell_2011_Proc.Natl.Acad.Sci.U.S.A_108_11217
Author(s) : O'Connell Motherway M , Zomer A , Leahy SC , Reunanen J , Bottacini F , Claesson MJ , O'Brien F , Flynn K , Casey PG , Munoz JA , Kearney B , Houston AM , O'Mahony C , Higgins DG , Shanahan F , Palva A , de Vos WM , Fitzgerald GF , Ventura M , O'Toole PW , van Sinderen D
Ref : Proc Natl Acad Sci U S A , 108 :11217 , 2011
Abstract : Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tad(2003)." Mutational analysis demonstrated that the tad(2003) gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria.
ESTHER : O'Connell_2011_Proc.Natl.Acad.Sci.U.S.A_108_11217
PubMedSearch : O'Connell_2011_Proc.Natl.Acad.Sci.U.S.A_108_11217
PubMedID: 21690406
Gene_locus related to this paper: bifln-c2gtr2

Title : Genome sequence of Chthoniobacter flavus Ellin428, an aerobic heterotrophic soil bacterium - Kant_2011_J.Bacteriol_193_2902
Author(s) : Kant R , van Passel MW , Palva A , Lucas S , Lapidus A , Glavina Del Rio T , Dalin E , Tice H , Bruce D , Goodwin L , Pitluck S , Larimer FW , Land ML , Hauser L , Sangwan P , de Vos WM , Janssen PH , Smidt H
Ref : Journal of Bacteriology , 193 :2902 , 2011
Abstract : Chthoniobacter flavus Ellin428 is the first isolate from the class Spartobacteria of the bacterial phylum Verrucomicrobia. C. flavus Ellin428 can metabolize many of the saccharide components of plant biomass but is incapable of growth on amino acids or organic acids other than pyruvate.
ESTHER : Kant_2011_J.Bacteriol_193_2902
PubMedSearch : Kant_2011_J.Bacteriol_193_2902
PubMedID: 21460085
Gene_locus related to this paper: 9bact-b4cw55 , 9bact-b4cx55 , 9bact-b4cy19 , 9bact-b4cyt2 , 9bact-b4cyu5 , 9bact-b4cza3 , 9bact-b4d1u0 , 9bact-b4d2s5 , 9bact-b4d373 , 9bact-b4d393 , 9bact-b4d914 , 9bact-b4daa7 , 9bact-b4dav5 , 9bact-b4db04 , 9bact-b4dc95 , 9bact-b4d3q8.1 , 9bact-b4d3q8.2

Title : Genome sequence of Pedosphaera parvula Ellin514, an aerobic Verrucomicrobial isolate from pasture soil - Kant_2011_J.Bacteriol_193_2900
Author(s) : Kant R , van Passel MW , Sangwan P , Palva A , Lucas S , Copeland A , Lapidus A , Glavina Del Rio T , Dalin E , Tice H , Bruce D , Goodwin L , Pitluck S , Chertkov O , Larimer FW , Land ML , Hauser L , Brettin TS , Detter JC , Han S , de Vos WM , Janssen PH , Smidt H
Ref : Journal of Bacteriology , 193 :2900 , 2011
Abstract : "Pedosphaera parvula" Ellin514 is an aerobically grown verrucomicrobial isolate from pasture soil. It is one of the few cultured representatives of subdivision 3 of the phylum Verrucomicrobia. Members of this group are widespread in terrestrial environments.
ESTHER : Kant_2011_J.Bacteriol_193_2900
PubMedSearch : Kant_2011_J.Bacteriol_193_2900
PubMedID: 21460084
Gene_locus related to this paper: 9bact-b9xah7.1 , 9bact-b9xah7.2 , 9bact-b9xba2 , 9bact-b9xfz4 , 9bact-b9xh19 , 9bact-b9xhn8 , 9bact-b9xj62 , 9bact-b9xjh1 , 9bact-b9xku6 , 9bact-b9xku8 , 9bact-b9xnx1 , 9bact-b9xp64 , 9bact-b9xp74

Title : Genome sequence of Victivallis vadensis ATCC BAA-548, an anaerobic bacterium from the phylum Lentisphaerae, isolated from the human gastrointestinal tract - van Passel_2011_J.Bacteriol_193_2373
Author(s) : van Passel MW , Kant R , Palva A , Lucas S , Copeland A , Lapidus A , Glavina Del Rio T , Dalin E , Tice H , Bruce D , Goodwin L , Pitluck S , Davenport KW , Sims D , Brettin TS , Detter JC , Han S , Larimer FW , Land ML , Hauser L , Kyrpides N , Ovchinnikova G , Richardson PP , de Vos WM , Smidt H , Zoetendal EG
Ref : Journal of Bacteriology , 193 :2373 , 2011
Abstract : Victivallis vadensis ATCC BAA-548 represents the first cultured representative from the novel phylum Lentisphaerae, a deep-branching bacterial lineage. Few cultured bacteria from this phylum are known, and V. vadensis therefore represents an important organism for evolutionary studies. V. vadensis is a strictly anaerobic sugar-fermenting isolate from the human gastrointestinal tract.
ESTHER : van Passel_2011_J.Bacteriol_193_2373
PubMedSearch : van Passel_2011_J.Bacteriol_193_2373
PubMedID: 21398537
Gene_locus related to this paper: 9bact-d1n3e0 , 9bact-d1n3w1 , 9bact-d1n5u2 , 9bact-d1n6r9 , 9bact-d1n8l2 , 9bact-d1n8z8 , 9bact-d1n9n1 , 9bact-d1n9u1 , 9bact-d1n752 , 9bact-d1n881 , 9bact-d1naa7 , 9bact-d1nb62 , 9bact-d1nbd5 , 9bact-d1nbg1 , 9bact-d1nbh9 , 9bact-d1nbv9

Title : Genome sequence of Lactobacillus amylovorus GRL1112 - Kant_2011_J.Bacteriol_193_789
Author(s) : Kant R , Paulin L , Alatalo E , de Vos WM , Palva A
Ref : Journal of Bacteriology , 193 :789 , 2011
Abstract : Lactobacillus amylovorus is a common member of the normal gastrointestinal tract (GIT) microbiota in pigs. Here, we report the genome sequence of L. amylovorus GRL1112, a porcine feces isolate displaying strong adherence to the pig intestinal epithelial cells. The strain is of interest, as it is a potential probiotic bacterium.
ESTHER : Kant_2011_J.Bacteriol_193_789
PubMedSearch : Kant_2011_J.Bacteriol_193_789
PubMedID: 21131492
Gene_locus related to this paper: lache-pepx , lache-prolinase , lacar-e4sin9 , lacar-e4sjn9

Title : Genome sequence of Lactobacillus amylovorus GRL1118, isolated from pig ileum - Kant_2011_J.Bacteriol_193_3147
Author(s) : Kant R , Paulin L , Alatalo E , de Vos WM , Palva A
Ref : Journal of Bacteriology , 193 :3147 , 2011
Abstract : Lactobacillus amylovorus is a common member of the beneficial microbiota present in the pig gastrointestinal tract. Here, we report the genome sequence of the surface layer (S-layer) protein-carrying and potentially probiotic strain L. amylovorus GRL1118, which was isolated from porcine ileum and which shows strong adherence to pig intestinal epithelial cells.
ESTHER : Kant_2011_J.Bacteriol_193_3147
PubMedSearch : Kant_2011_J.Bacteriol_193_3147
PubMedID: 21478337
Gene_locus related to this paper: lache-pepx , lache-prolinase

Title : Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human- mucus binding protein - Kankainen_2009_Proc.Natl.Acad.Sci.U.S.A_106_17193
Author(s) : Kankainen M , Paulin L , Tynkkynen S , von Ossowski I , Reunanen J , Partanen P , Satokari R , Vesterlund S , Hendrickx AP , Lebeer S , De Keersmaecker SC , Vanderleyden J , Hamalainen T , Laukkanen S , Salovuori N , Ritari J , Alatalo E , Korpela R , Mattila-Sandholm T , Lassig A , Hatakka K , Kinnunen KT , Karjalainen H , Saxelin M , Laakso K , Surakka A , Palva A , Salusjarvi T , Auvinen P , de Vos WM
Ref : Proc Natl Acad Sci U S A , 106 :17193 , 2009
Abstract : To unravel the biological function of the widely used probiotic bacterium Lactobacillus rhamnosus GG, we compared its 3.0-Mbp genome sequence with the similarly sized genome of L. rhamnosus LC705, an adjunct starter culture exhibiting reduced binding to mucus. Both genomes demonstrated high sequence identity and synteny. However, for both strains, genomic islands, 5 in GG and 4 in LC705, punctuated the colinearity. A significant number of strain-specific genes were predicted in these islands (80 in GG and 72 in LC705). The GG-specific islands included genes coding for bacteriophage components, sugar metabolism and transport, and exopolysaccharide biosynthesis. One island only found in L. rhamnosus GG contained genes for 3 secreted LPXTG-like pilins (spaCBA) and a pilin-dedicated sortase. Using anti-SpaC antibodies, the physical presence of cell wall-bound pili was confirmed by immunoblotting. Immunogold electron microscopy showed that the SpaC pilin is located at the pilus tip but also sporadically throughout the structure. Moreover, the adherence of strain GG to human intestinal mucus was blocked by SpaC antiserum and abolished in a mutant carrying an inactivated spaC gene. Similarly, binding to mucus was demonstrated for the purified SpaC protein. We conclude that the presence of SpaC is essential for the mucus interaction of L. rhamnosus GG and likely explains its ability to persist in the human intestinal tract longer than LC705 during an intervention trial. The presence of mucus-binding pili on the surface of a nonpathogenic Gram-positive bacterial strain reveals a previously undescribed mechanism for the interaction of selected probiotic lactobacilli with host tissues.
ESTHER : Kankainen_2009_Proc.Natl.Acad.Sci.U.S.A_106_17193
PubMedSearch : Kankainen_2009_Proc.Natl.Acad.Sci.U.S.A_106_17193
PubMedID: 19805152
Gene_locus related to this paper: lacrg-c7tei3 , lacrh-B2CZF3 , lacrh-b5qmk1 , lacrh-pepr , lacrh-pepx , lacrl-pip

Title : Complete genome sequence of Lactobacillus plantarum WCFS1 - Kleerebezem_2003_Proc.Natl.Acad.Sci.U.S.A_100_1990
Author(s) : Kleerebezem M , Boekhorst J , van Kranenburg R , Molenaar D , Kuipers OP , Leer R , Tarchini R , Peters SA , Sandbrink HM , Fiers MW , Stiekema W , Lankhorst RM , Bron PA , Hoffer SM , Groot MN , Kerkhoven R , De Vries M , Ursing B , de Vos WM , Siezen RJ
Ref : Proc Natl Acad Sci U S A , 100 :1990 , 2003
Abstract : The 3,308,274-bp sequence of the chromosome of Lactobacillus plantarum strain WCFS1, a single colony isolate of strain NCIMB8826 that was originally isolated from human saliva, has been determined, and contains 3,052 predicted protein-encoding genes. Putative biological functions could be assigned to 2,120 (70%) of the predicted proteins. Consistent with the classification of L. plantarum as a facultative heterofermentative lactic acid bacterium, the genome encodes all enzymes required for the glycolysis and phosphoketolase pathways, all of which appear to belong to the class of potentially highly expressed genes in this organism, as was evident from the codon-adaptation index of individual genes. Moreover, L. plantarum encodes a large pyruvate-dissipating potential, leading to various end-products of fermentation. L. plantarum is a species that is encountered in many different environmental niches, and this flexible and adaptive behavior is reflected by the relatively large number of regulatory and transport functions, including 25 complete PTS sugar transport systems. Moreover, the chromosome encodes >200 extracellular proteins, many of which are predicted to be bound to the cell envelope. A large proportion of the genes encoding sugar transport and utilization, as well as genes encoding extracellular functions, appear to be clustered in a 600-kb region near the origin of replication. Many of these genes display deviation of nucleotide composition, consistent with a foreign origin. These findings suggest that these genes, which provide an important part of the interaction of L. plantarum with its environment, form a lifestyle adaptation region in the chromosome.
ESTHER : Kleerebezem_2003_Proc.Natl.Acad.Sci.U.S.A_100_1990
PubMedSearch : Kleerebezem_2003_Proc.Natl.Acad.Sci.U.S.A_100_1990
PubMedID: 12566566
Gene_locus related to this paper: lacpl-EST1 , lacpl-EST2 , lacpl-HPO , lacpl-LP.0461 , lacpl-LP.0618 , lacpl-LP.0796 , lacpl-LP.0973 , lacpl-LP.1002 , lacpl-LP.1124 , lacpl-LP.1156 , lacpl-LP.1165 , lacpl-LP.1760 , lacpl-LP.1774 , lacpl-LP.1935 , lacpl-LP.2519 , lacpl-LP.2586 , lacpl-LP.2620 , lacpl-LP.2631 , lacpl-LP.2737 , lacpl-LP.2923 , lacpl-LP.2953 , lacpl-LP.3205 , lacpl-LP.3265 , lacpl-LP.3341 , lacpl-LP.3393 , lacpl-LP.3561 , lacpl-LP.3562 , lacpl-PEPI , lacpl-PEPR1 , lacpl-PEPR2 , lacpl-pepx , lacpl-tanL

Title : Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates - van der Meer_1991_J.Bacteriol_173_2425
Author(s) : van der Meer JR , Eggen RI , Zehnder AJ , de Vos WM
Ref : Journal of Bacteriology , 173 :2425 , 1991
Abstract : Pseudomonas sp. strain P51 contains two gene clusters located on catabolic plasmid pP51 that encode the degradation of chlorinated benzenes. The nucleotide sequence of a 5,499-bp region containing the chlorocatechol-oxidative gene cluster tcbCDEF was determined. The sequence contained five large open reading frames, which were all colinear. The functionality of these open reading frames was studied with various Escherichia coli expression systems and by analysis of enzyme activities. The first gene, tcbC, encodes a 27.5-kDa protein with chlorocatechol 1,2-dioxygenase activity. The tcbC gene is followed by tcbD, which encodes cycloisomerase II (39.5 kDa); a large open reading frame (ORF3) with an unknown function; tcbE, which encodes hydrolase II (25.8 kDa); and tcbF, which encodes a putative trans-dienelactone isomerase (37.5 kDa). The tcbCDEF gene cluster showed strong DNA homology (between 57.6 and 72.1% identity) and an organization similar to that of other known plasmid-encoded operons for chlorocatechol metabolism, e.g., clcABD of Pseudomonas putida and tfdCDEF of Alcaligenes eutrophus JMP134. The identity between amino acid sequences of functionally related enzymes of the three operons varied between 50.6 and 75.7%, with the tcbCDEF and tfdCDEF pair being the least similar of the three. Measurements of the specific activities of chlorocatechol 1,2-dioxygenases encoded by tcbC, clcA, and tfdC suggested that a specialization among type II enzymes has taken place. TcbC preferentially converts 3,4-dichlorocatechol relative to other chlorinated catechols, whereas TfdC has a higher activity toward 3,5-dichlorocatechol. ClcA takes an intermediate position, with the highest activity level for 3-chlorocatechol and the second-highest level for 3,5-dichlorocatechol.
ESTHER : van der Meer_1991_J.Bacteriol_173_2425
PubMedSearch : van der Meer_1991_J.Bacteriol_173_2425
PubMedID: 2013566
Gene_locus related to this paper: alceu-CBNC