Ohe H

References (1)

Title : Direct determination of E2020 enantiomers in plasma by liquid chromatography-mass spectrometry and column-switching techniques - Matsui_1995_J.Chromatogr.A_694_209
Author(s) : Matsui K , Oda Y , Ohe H , Tanaka S , Asakawa N
Ref : Journal of Chromatography A , 694 :209 , 1995
Abstract : High-performance liquid chromatography with column switching and mass spectrometry (MS) was applied to the on-line determination and resolution of the enantiomers of E2020 (acetylcholinesterase inhibitor) in plasma. This system employs two avidin columns and fast atom bombardment (FAB)-MS. A plasma sample was injected directly into an avidin trapping column (10 mm x 4.0 mm I.D.). The plasma protein was washed out from the trapping column immediately while E2020 was retained. After the column-switching procedure, E2020 was separated enantioselectivity in an avidin analytical column. The separated E2020 enantiomers were specifically detected by FAB-MS without interference from metabolites of E2020 and plasma constituents. The limit of quantification for each enantiomer of E2020 in plasma was 1.0 ng/ml and the intra- and inter-assay relative standard deviations for the method were less than 5.2%. The assay was validated for enantioselective pharmacokinetic studies in the dog.
ESTHER : Matsui_1995_J.Chromatogr.A_694_209
PubMedSearch : Matsui_1995_J.Chromatogr.A_694_209
PubMedID: 7719466