Oliveira DM

References (3)

Title : Feruloyl esterase activity and its role in regulating the feruloylation of maize cell walls - Oliveira_2020_Plant.Physiol.Biochem_156_49
Author(s) : Oliveira DM , Mota TR , Salatta FV , de Almeida GHG , Olher VGA , Oliveira MAS , Marchiosi R , Ferrarese-Filho O , Dos Santos WD
Ref : Plant Physiol Biochem , 156 :49 , 2020
Abstract : Cell walls of grasses have ferulic acid (FA) ester-linked to the arabinosyl substitutions of arabinoxylan (AX). Feruloyl esterases (FAE) are carboxylic acid esterases that release FA from cell walls and synthetic substrates. Despite the importance of FA for cell wall recalcitrance and in response to biotic and abiotic stresses, the physiological function of plant FAEs remains unclear. Here, we developed a simple method for the determination of FAE activity (ZmFAE) in maize using the total protein extract and investigated its role in regulating the feruloylation of cell wall. The method includes a single protein extraction and enzymatic reaction with protein concentration as low as 65 mug at 35 degC for 30 min, using methyl ferulate as the substrate. The methodology allowed the determination of the apparent K(m) (392.82 muM) and V(max) (79.15 pkat mg(-1) protein). We also found that ZmFAE activity was correlated (r = 0.829) with the levels of FA in seedling roots, plant roots and leaves of maize. Furthermore, the exposure to osmotic stress resulted in a 50% increase in ZmFAE activity in seedling roots. These data suggest that FAE-catalyzed reaction is important for cell wall feruloylation during plant development and in response to abiotic stress. We conclude proposing a model for the feruloylation and deferuloylation of AX, which explains the role of FAE in regulating the levels of ester-linked FA. Our model might orient further studies investigating the role of plant FAEs and assist strategies for genetic engineering of grasses to obtain plants with reduced biomass recalcitrance.
ESTHER : Oliveira_2020_Plant.Physiol.Biochem_156_49
PubMedSearch : Oliveira_2020_Plant.Physiol.Biochem_156_49
PubMedID: 32906021

Title : Feruloyl esterases: Biocatalysts to overcome biomass recalcitrance and for the production of bioactive compounds - Oliveira_2019_Bioresour.Technol_278_408
Author(s) : Oliveira DM , Mota TR , Oliva B , Segato F , Marchiosi R , Ferrarese-Filho O , Faulds CB , Dos Santos WD
Ref : Bioresour Technol , 278 :408 , 2019
Abstract : Ferulic acid and its hydroxycinnamate derivatives represent one of the most abundant forms of low molecular weight phenolic compounds in plant biomass. Feruloyl esterases are part of a microorganism's plant cell wall-degrading enzymatic arsenal responsible for cleaving insoluble wall-bound hydroxycinnamates and soluble cytosolic conjugates. Stimulated by industrial requirements, accelerating scientific discoveries and knowledge transfer, continuous improvement efforts have been made to identify, create and repurposed biocatalysts dedicated to plant biomass conversion and biosynthesis of high-added value molecules. Here we review the basic knowledge and recent advances in biotechnological characteristics and the gene content encoding for feruloyl esterases. Information about several enzymes is systematically organized according to their function, biochemical properties, substrate specificity, and biotechnological applications. This review contributes to further structural, functional, and biotechnological R&D both for obtaining hydroxycinnamates from agricultural by-products as well as for lignocellulose biomass treatments aiming for production of bioethanol and other derivatives of industrial interest.
ESTHER : Oliveira_2019_Bioresour.Technol_278_408
PubMedSearch : Oliveira_2019_Bioresour.Technol_278_408
PubMedID: 30704902

Title : Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains - Ruiz_2011_PLoS.One_6_e18551
Author(s) : Ruiz JC , D'Afonseca V , Silva A , Ali A , Pinto AC , Santos AR , Rocha AA , Lopes DO , Dorella FA , Pacheco LG , Costa MP , Turk MZ , Seyffert N , Moraes PM , Soares SC , Almeida SS , Castro TL , Abreu VA , Trost E , Baumbach J , Tauch A , Schneider MP , McCulloch J , Cerdeira LT , Ramos RT , Zerlotini A , Dominitini A , Resende DM , Coser EM , Oliveira LM , Pedrosa AL , Vieira CU , Guimaraes CT , Bartholomeu DC , Oliveira DM , Santos FR , Rabelo EM , Lobo FP , Franco GR , Costa AF , Castro IM , Dias SR , Ferro JA , Ortega JM , Paiva LV , Goulart LR , Almeida JF , Ferro MI , Carneiro NP , Falcao PR , Grynberg P , Teixeira SM , Brommonschenkel S , Oliveira SC , Meyer R , Moore RJ , Miyoshi A , Oliveira GC , Azevedo V
Ref : PLoS ONE , 6 :e18551 , 2011
Abstract : BACKGROUND: Corynebacterium pseudotuberculosis, a gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity. METHODOLOGY AND FINDINGS: We characterized two C. pseudotuberculosis genomes (Cp1002, isolated from goats; and CpC231, isolated from sheep). Analysis of the predicted genomes showed high similarity in genomic architecture, gene content and genetic order. When C. pseudotuberculosis was compared with other Corynebacterium species, it became evident that this pathogenic species has lost numerous genes, resulting in one of the smallest genomes in the genus. Other differences that could be part of the adaptation to pathogenicity include a lower GC content, of about 52%, and a reduced gene repertoire. The C. pseudotuberculosis genome also includes seven putative pathogenicity islands, which contain several classical virulence factors, including genes for fimbrial subunits, adhesion factors, iron uptake and secreted toxins. Additionally, all of the virulence factors in the islands have characteristics that indicate horizontal transfer.
CONCLUSIONS: These particular genome characteristics of C. pseudotuberculosis, as well as its acquired virulence factors in pathogenicity islands, provide evidence of its lifestyle and of the pathogenicity pathways used by this pathogen in the infection process. All genomes cited in this study are available in the NCBI Genbank database (http:\/\/www.ncbi.nlm.nih.gov/genbank/) under accession numbers CP001809 and CP001829.
ESTHER : Ruiz_2011_PLoS.One_6_e18551
PubMedSearch : Ruiz_2011_PLoS.One_6_e18551
PubMedID: 21533164
Gene_locus related to this paper: corpf-d8knc3 , corpf-d8kpq1 , corpf-d8kps5 , corpf-d8kq17 , corp2-d9qa08 , corps-h8lrl6 , corp1-d9q3x2 , corp1-d9q7e0