Robertson WD

References (4)

Title : Protein crystals IR laser ablated from aqueous solution at high speed retain their diffractive properties: applications in high-speed serial crystallography - Schulz_2017_J.Appl.Cryst_50_1773
Author(s) : Schulz EC , Kaub J , Busse F , Mehrabi P , Muller-Werkmeister HM , Pai EF , Robertson WD , Miller RJD
Ref : Journal of Applied Crystallography , 50 :1773 , 2017
Abstract : In order to utilize the high repetition rates now available at X-ray free-electron laser sources for serial crystallography, methods must be developed to softly deliver large numbers of individual microcrystals at high repetition rates and high speeds. Picosecond infrared laser (PIRL) pulses, operating under desorption by impulsive vibrational excitation (DIVE) conditions, selectively excite the OH vibrational stretch of water to directly propel the excited volume at high speed with minimized heating effects, nucleation formation or cavitation-induced shock waves, leaving the analytes intact and undamaged. The soft nature and laser-based sampling flexibility provided by the technique make the PIRL system an interesting crystal delivery approach for serial crystallography. This paper demonstrates that protein crystals extracted directly from aqueous buffer solution via PIRL-DIVE ablation retain their diffractive properties and can be usefully exploited for structure determination at synchrotron sources. The remaining steps to implement the technology for high-speed serial femtosecond crystallography, such as single-crystal localization, high-speed sampling and synchronization, are described. This proof-of-principle experiment demonstrates the viability of a new laser-based high-speed crystal delivery system without the need for liquid-jet injectors or fixed-target mounting solutions.
ESTHER : Schulz_2017_J.Appl.Cryst_50_1773
PubMedSearch : Schulz_2017_J.Appl.Cryst_50_1773
PubMedID:
Gene_locus related to this paper: rhopa-q6nam1

Title : Ixodicidal resistance in Boophilus microplus (Canestrini) in the Republic of South Africa and Transkei - Baker_1979_J.S.Afr.Vet.Assoc_50_296
Author(s) : Baker JA , Jordaan JO , Robertson WD
Ref : J S Afr Vet Assoc , 50 :296 , 1979
Abstract : A series of in vitro trials using unfed larvae and fully fed adult ticks confirmed ixodicidal resistance in the one-host Pantropical Blue Tick, Boophilus microplus (Canestrini). Fifty-seven of 64 field isolates were resistant to arsenic; 10 of 56 were resistant to toxaphene; 1 of 5 were resistant to lindane; 3 of 5 were resistant to dieldrin; 3 of 19 were resistant to DDT and 8 of 55 were resistant to the organophosphorus ixodicide, dioxathion. One of the field isolates resistant to dioxathion was also highly resistant to the carbamate, carbaryl, and to the organophosphorus ixodicides benoxophos and diazinon. A second was resistant to the organophosphorus ixodicides benoxophos, diazinon, carbophenothion, dicrotophos, ethion, fenitrothion and quintiofos. Low levels of resistance, less than 3X, were shown to chlorfenvinphos and coumaphos. No resistance was shown to chlorpyrifos, bromophos ethyl or the diamidine ixodicide, amitraz. In hand-spraying trials no variation in the susceptibility of an organophosphorus resistant strain or the susceptible laboratory strain to amitraz was observed. This is the first recorded resistance to ixodicides by B. microplus in Africa.
ESTHER : Baker_1979_J.S.Afr.Vet.Assoc_50_296
PubMedSearch : Baker_1979_J.S.Afr.Vet.Assoc_50_296
PubMedID: 553968

Title : Resistance to certain organophosphorus ixodicides in the bont tick, Amblyomma hebraeum Koch, in the Republic of South Africa and Swaziland - Baker_1978_J.S.Afr.Vet.Assoc_49_337
Author(s) : Baker JA , Miles JO , Robertson WD
Ref : J S Afr Vet Assoc , 49 :337 , 1978
Abstract : A series of in vitro trails confirmed resistance in the three-host tick, Amblyomma hebraeum Koch, to the organophosphorus ixodicides, dioxathion, chlorfenvinphos, quintiofos, bromophos-ethyl and a dioxathion/chlorfenvinphos combination. The results of a routine survey showed that a resistance factor of greater than 100x was present in the offspring of ticks in six of 115 field isolates tested. These six isolates were confined to the lowveld areas of the province of Natal, Republic of South Africa, and Swaziland. Ticks in a further 59 isolates were less susceptible than the reference strain. Resistance was present in all three tick instars. This is the first recorded resistance to the organophosphorus group of ixodicides by multi-host ticks in Africa.
ESTHER : Baker_1978_J.S.Afr.Vet.Assoc_49_337
PubMedSearch : Baker_1978_J.S.Afr.Vet.Assoc_49_337
PubMedID: 752082

Title : The current status of resistance to organophosphorus ixodicides by the blue tick, Boophilus Decoloratus (Koch) in the Republic of South Africa and Transkei - Baker_1978_J.S.Afr.Vet.Assoc_49_327
Author(s) : Baker JA , Miles JO , Robertson WD , Stanford GD , Taylor RJ
Ref : J S Afr Vet Assoc , 49 :327 , 1978
Abstract : In a four year survey 253 isolates of Boophilus decoloratus from the Republic of South Africa and Transkei were tested in the laboratory for their susceptibility to certain organophosphorus ixodicides. Fifty-seven of these isolates were susceptible to organophosphorus compounds, 88 isolates showed a range of susceptibility between that of the susceptible laboratory strain and a strain having a resistance greater than that for the Berlin type strain. A field trial confirmed degrees of resistance shown in the laboratory to the organophosphorus ixodicides benoxophos, chlorfenvinphos, dioxathion, dicrotophos, quintiofos and chlorfenvinphos/dioxathion.
ESTHER : Baker_1978_J.S.Afr.Vet.Assoc_49_327
PubMedSearch : Baker_1978_J.S.Afr.Vet.Assoc_49_327
PubMedID: 752081