rhopa-q6nam1

Rhodopseudomonas palustris (and strains BisB5; TIE-1) RPA1163 possible epoxide hydrolase

Comment

Relationship

Family : Haloacetate_dehalogenase

Block : X

Position in NCBI Life Tree : Rhodopseudomonas palustris

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Proteobacteria N E > Alphaproteobacteria N E > Rhizobiales N E > Bradyrhizobiaceae N E > Rhodopseudomonas N E > Rhodopseudomonas palustris N E

Molecular evidence

No mutation

6QHT - Time resolved structural analysis of the full turnover of an enzyme - 376 ms 5NYV - Crystal structure determination from picosecond infrared laser ablated protein crystals by serial synchrotron crystallography 6QHQ - Time resolved structural analysis of the full turnover of an enzyme - 1128 ms 6QHV - Time resolved structural analysis of the full turnover of an enzyme - 100 ms 6QHU - Time resolved structural analysis of the full turnover of an enzyme - 100 ms 6QHX - Time resolved structural analysis of the full turnover of an enzyme - 6156 ms 6QHW - Time resolved structural analysis of the full turnover of an enzyme - 4512 ms 6QHZ - Time resolved structural analysis of the full turnover of an enzyme - 6788 ms 6QHY - Time resolved structural analysis of the full turnover of an enzyme - 100 ms 6QHS - Time resolved structural analysis of the full turnover of an enzyme - 13536 ms 6QHP - Time resolved structural analysis of the full turnover of an enzyme - 2256 ms covalent intermediate 1 6QI1 - Time resolved structural analysis of the full turnover of an enzyme - 12312 ms 6QI0 - Time resolved structural analysis of the full turnover of an enzyme - 9024 ms 6QI3 - Time resolved structural analysis of the full turnover of an enzyme - 27072 ms 6QI2 - Time resolved structural analysis of the full turnover of an enzyme - 13536 ms 7A43 - Fluoroacetate Dehalogenase of Rhodopseudomonas palustris measured by serial femtosecond crystallography 7A42 - Fluoroacetate Dehalogenase of Rhodopseudomonas palustris measured by serial synchrotron crystallography 6N00 - Fluoroacetate dehalogenase, room temperature structure, using last 1 degree of total 3 degree oscillation and 144 kGy dose 6MZZ - Fluoroacetate dehalogenase, room temperature structure, using first 1 degree of total 3 degree oscillation 6MUH - Fluoroacetate dehalogenase, room temperature structure solved by serial 1 degree oscillation crystallography 6MUY - Fluoroacetate dehalogenase, room temperature structure solved by serial 3 degree oscillation crystallography 6GXH - Rhodopseudomonas palustris Fluoroacetate dehalogenase.: FAcD 0MS after reaction initiation 6GXT - Rhodopseudomonas palustris Fluoroacetate dehalogenase : FAcD2052MS after reaction initiation 6GXL - Rhodopseudomonas palustris Fluoroacetate dehalogenase : RADDAM2 6GXF - Rhodopseudomonas palustris Fluoroacetate dehalogenase : RADDAM1 6GXD - Rhodopseudomonas palustris Fluoroacetate dehalogenase : FAcD752MS after reaction initiation 6FSX - Rhodopseudomonas palustris (strain ATCC BAA-98 \/ CGA009) Fluoroacetate dehalogenase 6QKU - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Tyr219Phe - Chloroacetate soaked 2hr 6QKT - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Tyr219Phe - Fluoroacetate soaked 24hr - Glycolate bound 6QKW - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Tyr219Phe - Fluoroacetate soaked 2hr 6QKS - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Tyr219Phe - Apo 5O2I - Crystal structure of the Fluoroacetate Dehalogenase RPA1163: An efficient setup for fixed-target, time-resolved serial crystallography with optical excitation 5O2G - Crystal structure of the Fluoroacetate Dehalogenase RPA1163: determination from picosecond infrared laser ablated protein crystals by serial synchrotron crystallography 5T4T - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Asp110Asn - Apo No Halide 5SWN - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Asp110Asn\/Glycolate - Cocrystallized 5K3A - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - His280Asn\/Fluoroacetate - Cocrystallized - Both Protomers Reacted with Ligand 5K3B - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Asp110Asn\/Chloroacetate - Cocrystallized 5K3C - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - WT\/5-Fluorotryptophan 5K3D - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - WT\/Apo - No Halide 5K3F - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - His280Asn\/Fluoroacetate - Cocrystallized - Single Protomer Reacted with Ligand 5K3E - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Asp110Asn\/Glycolate - Cocrystallized 3R3U - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - WT\/apo 3R3V - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Asp110Asn\/Fluoroacetate 3R3W - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Asp110Asn\/Chloroacetate 3R3X - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Asp110Asn\/Bromoacetate 3R3Y - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - His280Asn\/Fluoroacetate 3R3Z - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - WT\/Glycolate 3R40 - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Asp110Asn\/apo 3R41 - Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - His280Asn\/apo

No kinetic

No disease

Database

Sequence

Peptide

MPDLADLFPG FGSEWINTSS GRIFARVGGD GPPLLLLHGF PQTHVMWHRV APKLAERFKV IVADLPGYGW SDMPESDEQH TPYTKRAMAK QLIEAMEQLG HVHFALAGHD RGARVSYRLA LDSPGRLSKL AVLDILPTYE YWQRMNRAYA LKIYHWSFLA QPAPLPENLL GGDPDFYVKA KLASWTRAGD LSAFDPRAVE HYRIAFADPM RRHVMCEDYR AGAYADFEHD KIDVEAGNKI PVPMLALWGA SGIAQSAATP LDVWRKWASD VQGAPIESGH FLPEEAPDQT AEALVRFFSA AP

References (10)

Title : Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison - Mehrabi_2021_Sci.Adv_7_eabf1380
Author(s) : Mehrabi P , Bucker R , Bourenkov G , Ginn HM , von Stetten D , Muller-Werkmeister HM , Kuo A , Morizumi T , Eger BT , Ou WL , Oghbaey S , Sarracini A , Besaw JE , Pare-Labrosse O , Meier S , Schikora H , Tellkamp F , Marx A , Sherrell DA , Axford D , Owen RL , Ernst OP , Pai EF , Schulz EC , Miller RJD
Ref : Sci Adv , 7 : , 2021
PubMedID: 33731353
Gene_locus related to this paper: rhopa-q6nam1

Title : Time-resolved crystallography reveals allosteric communication aligned with molecular breathing - Mehrabi_2019_Science_365_1167
Author(s) : Mehrabi P , Schulz EC , Dsouza R , Muller-Werkmeister HM , Tellkamp F , Miller RJD , Pai EF
Ref : Science , 365 :1167 , 2019
PubMedID: 31515393
Gene_locus related to this paper: rhopa-q6nam1

Title : Substrate-Based Allosteric Regulation of a Homodimeric Enzyme - Mehrabi_2019_J.Am.Chem.Soc_141_11540
Author(s) : Mehrabi P , Di Pietrantonio C , Kim TH , Sljoka A , Taverner K , Ing C , Kruglyak N , Pomes R , Pai EF , Prosser RS
Ref : Journal of the American Chemical Society , 141 :11540 , 2019
PubMedID: 31188575
Gene_locus related to this paper: rhopa-q6nam1

Title : Fixed-target serial oscillation crystallography at room temperature - Wierman_2019_IUCrJ_6_305
Author(s) : Wierman JL , Pare-Labrosse O , Sarracini A , Besaw JE , Cook MJ , Oghbaey S , Daoud H , Mehrabi P , Kriksunov I , Kuo A , Schuller DJ , Smith S , Ernst OP , Szebenyi DME , Gruner SM , Miller RJD , Finke AD
Ref : IUCrJ , 6 :305 , 2019
PubMedID: 30867928
Gene_locus related to this paper: rhopa-q6nam1

Title : The hit-and-return system enables efficient time-resolved serial synchrotron crystallography - Schulz_2018_Nat.Methods_15_901
Author(s) : Schulz EC , Mehrabi P , Muller-Werkmeister HM , Tellkamp F , Jha A , Stuart W , Persch E , De Gasparo R , Diederich F , Pai EF , Miller RJD
Ref : Nat Methods , 15 :901 , 2018
PubMedID: 30377366
Gene_locus related to this paper: rhopa-q6nam1

Title : The role of dimer asymmetry and protomer dynamics in enzyme catalysis - Kim_2017_Science_355_
Author(s) : Kim TH , Mehrabi P , Ren Z , Sljoka A , Ing C , Bezginov A , Ye L , Pomes R , Prosser RS , Pai EF
Ref : Science , 355 : , 2017
PubMedID: 28104837
Gene_locus related to this paper: rhopa-q6nam1

Title : Protein crystals IR laser ablated from aqueous solution at high speed retain their diffractive properties: applications in high-speed serial crystallography - Schulz_2017_J.Appl.Cryst_50_1773
Author(s) : Schulz EC , Kaub J , Busse F , Mehrabi P , Muller-Werkmeister HM , Pai EF , Robertson WD , Miller RJD
Ref : Journal of Applied Crystallography , 50 :1773 , 2017
PubMedID:
Gene_locus related to this paper: rhopa-q6nam1

Title : Mapping the reaction coordinates of enzymatic defluorination - Chan_2011_J.Am.Chem.Soc_133_7461
Author(s) : Chan PW , Yakunin AF , Edwards EA , Pai EF
Ref : Journal of the American Chemical Society , 133 :7461 , 2011
PubMedID: 21510690
Gene_locus related to this paper: rhopa-q6nam1

Title : Sequence- and activity-based screening of microbial genomes for novel dehalogenases - Chan_2010_Microb.Biotechnol_3_107
Author(s) : Chan WY , Wong M , Guthrie J , Savchenko AV , Yakunin AF , Pai EF , Edwards EA
Ref : Microb Biotechnol , 3 :107 , 2010
PubMedID: 21255311
Gene_locus related to this paper: rhopa-q6nam1