Sokolov SL

References (2)

Title : Complete Genome Sequence of Steroid-Transforming Nocardioides simplex VKM Ac-2033D - Shtratnikova_2015_Genome.Announc_3_e01406
Author(s) : Shtratnikova VY , Schelkunov MI , Pekov YA , Fokina VV , Logacheva MD , Sokolov SL , Bragin EY , Ashapkin VV , Donova MV
Ref : Genome Announc , 3 : , 2015
Abstract : Nocardioides simplex VKM Ac-2033D is an effective microbial catalyst for 3-ketosteroid 1(2)-dehydrogenation, and it is capable of effective reduction of carbonyl groups at C-17 and C-20, hydrolysis of acetylated steroids, and utilization of natural sterols. Here, the complete genome sequence is reported. An array of genes related to steroid metabolic pathways have been identified.
ESTHER : Shtratnikova_2015_Genome.Announc_3_e01406
PubMedSearch : Shtratnikova_2015_Genome.Announc_3_e01406
PubMedID: 25573942
Gene_locus related to this paper: nocsi-a0a0a1dtc2 , nocsi-a0a0c5xh38 , nocsi-a0a0a1dua8

Title : Comparative analysis of genes encoding key steroid core oxidation enzymes in fast-growing Mycobacterium spp. strains - Bragin_2013_J.Steroid.Biochem.Mol.Biol_138_41
Author(s) : Bragin EY , Shtratnikova VY , Dovbnya DV , Schelkunov MI , Pekov YA , Malakho SG , Egorova OV , Ivashina TV , Sokolov SL , Ashapkin VV , Donova MV
Ref : J Steroid Biochem Mol Biol , 138 :41 , 2013
Abstract : A comparative genome analysis of Mycobacterium spp. VKM Ac-1815D, 1816D and 1817D strains used for efficient production of key steroid intermediates (androst-4-ene-3,17-dione, AD, androsta-1,4-diene-3,17-dione, ADD, 9alpha-hydroxy androst-4-ene-3,17-dione, 9-OH-AD) from phytosterol has been carried out by deep sequencing. The assembled contig sequences were analyzed for the presence putative genes of steroid catabolism pathways. Since 3-ketosteroid-9alpha-hydroxylases (KSH) and 3-ketosteroid-Delta(1)-dehydrogenase (Delta(1) KSTD) play key role in steroid core oxidation, special attention was paid to the genes encoding these enzymes. At least three genes of Delta(1) KSTD (kstD), five genes of KSH subunit A (kshA), and one gene of KSH subunit B of 3-ketosteroid-9alpha-hydroxylases (kshB) have been found in Mycobacterium sp. VKM Ac-1817D. Strains of Mycobacterium spp. VKM Ac-1815D and 1816D were found to possess at least one kstD, one kshB and two kshA genes. The assembled genome sequence of Mycobacterium sp. VKM Ac-1817D differs from those of 1815D and 1816D strains, whereas these last two are nearly identical, differing by 13 single nucleotide substitutions (SNPs). One of these SNPs is located in the coding region of a kstD gene and corresponds to an amino acid substitution Lys (135) in 1816D for Ser (135) in 1815D. The findings may be useful for targeted genetic engineering of the biocatalysts for biotechnological application.
ESTHER : Bragin_2013_J.Steroid.Biochem.Mol.Biol_138_41
PubMedSearch : Bragin_2013_J.Steroid.Biochem.Mol.Biol_138_41
PubMedID: 23474435
Gene_locus related to this paper: mycfo-k0vcg0 , mycfo-a0a0n9yhd4