Tabandeh F

References (2)

Title : Protein Engineering of Bacillus thermocatenulatus Lipase via Deletion of the alpha5 Helix - Goodarzi_2014_Appl.Biochem.Biotechnol_174_339
Author(s) : Goodarzi N , Karkhane AA , Mirlohi A , Tabandeh F , Torktas I , Aminzadeh S , Yakhchali B , Shamsara M , Ghafouri MA
Ref : Appl Biochem Biotechnol , 174 :339 , 2014
Abstract : Lipases from Bacillus thermocatenulatus are a member of superfamily of alpha/beta hydrolase, but there are structural differences between them. In this work, we focused on the alpha5 helix of B. thermocatenulatus lipase (BTL2) which is absent in canonical alpha/beta hydrolase fold. In silico study showed that the alpha5 helix is a region that causes disorder in BTL2 protein. So, the alpha5 helix (residues 131 to 150) has been deleted from the B. thermocatenulatus lipase gene (BTL2) and the remain (Deltaalpha5-BTL2) has been expressed in Pichia pastoris yeast. The alpha5 deletion results in increase of enzyme-specific activity in the presence of tributyrin, tricaproin, tricaprylin, tricaprin, trilaurin, and olive oil (C18) substrates by 1.4-, 1.7-, 2.0-, 1.2-, 1.75-, and 1.95-fold, respectively. Also, deletion leads to increase in enzyme activity in different temperatures and pHs, whereas it did not significantly affect on enzyme activity in the presence of organic solvents, metal ions, and detergents.
ESTHER : Goodarzi_2014_Appl.Biochem.Biotechnol_174_339
PubMedSearch : Goodarzi_2014_Appl.Biochem.Biotechnol_174_339
PubMedID: 25064134
Gene_locus related to this paper: bactc-lipas

Title : Isolation and identification of a lipase producing Bacillus sp. from soil - Heravi_2008_Pak.J.Biol.Sci_11_740
Author(s) : Heravi KM , Eftekhar F , Yakhchali B , Tabandeh F
Ref : Pak J Biol Sci , 11 :740 , 2008
Abstract : Lipase production in an indigenous lipolytic Bacillus sp. was detected in media containing Tributyrin-Tween 80 and Rhodamine B-Olive oil. The statistical Taguchi model was used to predict the optimum experimental conditions for bacterial growth and lipase production. Partial optimization was carried out for selection of salt base, oil, glucose, NH4Cl and yeast extract concentrations, inoculum density, pH and agitation. Maximum lipase activity was detected in the cell free supernatants of cultures grown in a medium containing 10 g L(-1) yeast extract, 15 g L(-1) NH4Cl, 3 g L(-1) K2HPO4, 1 g L(-1) KH2PO4, 0.1 g L(-1) MgSO4 x 7H2O, 2 g L(-1) glucose, 0.6 mM MgCl2 and 15 ml L(-1) olive oil, pH 8.5 at 30 degrees C for 24 h and low agitation. The amount oflipase produced in the designed medium was in agreement with the predicted values by the statistical method. 16S rRNA cloning and sequencing identified the test organism as Bacillus pumilus.
ESTHER : Heravi_2008_Pak.J.Biol.Sci_11_740
PubMedSearch : Heravi_2008_Pak.J.Biol.Sci_11_740
PubMedID: 18819570