Yanai S

References (2)

Title : NOAEL-dose of a neonicotinoid pesticide, clothianidin, acutely induce anxiety-related behavior with human-audible vocalizations in male mice in a novel environment - Hirano_2018_Toxicol.Lett_282_57
Author(s) : Hirano T , Yanai S , Takada T , Yoneda N , Omotehara T , Kubota N , Minami K , Yamamoto A , Mantani Y , Yokoyama T , Kitagawa H , Hoshi N
Ref : Toxicol Lett , 282 :57 , 2018
Abstract : Neonicotinoids are novel systemic pesticides acting as agonists on the nicotinic acetylcholine receptors (nAChRs) of insects. Experimental studies have revealed that neonicotinoids pose potential risks for the nervous systems of non-target species, but the brain regions responsible for their behavioral effects remain incompletely understood. This study aimed to assess the neurobehavioral effects of clothianidin (CTD), a later neonicotinoid developed in 2001 and widely used worldwide, and to explore the target regions of neonicotinoids in the mammalian brain. A single-administration of 5 or 50mg/kg CTD to male C57BL/6N mice at or below the no-observed-adverse-effect level (NOAEL) induced an acute increase in anxiety during the elevated plus-maze test. In addition, mice in the CTD-administered group spontaneously emitted human-audible vocalizations (4-16kHz), which are behavioral signs of aversive emotions, and showed increased numbers of c-fos immunoreactive cells in the paraventricular thalamic nucleus and dentate gyrus of the hippocampus. In conclusion, mice exposed to NOAEL-dose CTD would be rendered vulnerable to a novel environment via the activation of thalamic and hippocampal regions related to stress responses. These findings should provide critical insight into the neurobehavioral effects of neonicotinoids on mammals.
ESTHER : Hirano_2018_Toxicol.Lett_282_57
PubMedSearch : Hirano_2018_Toxicol.Lett_282_57
PubMedID: 29030271

Title : Evidence for at least six Hox clusters in the Japanese lamprey (Lethenteron japonicum) - Mehta_2013_Proc.Natl.Acad.Sci.U.S.A_110_16044
Author(s) : Mehta TK , Ravi V , Yamasaki S , Lee AP , Lian MM , Tay BH , Tohari S , Yanai S , Tay A , Brenner S , Venkatesh B
Ref : Proc Natl Acad Sci U S A , 110 :16044 , 2013
Abstract : Cyclostomes, comprising jawless vertebrates such as lampreys and hagfishes, are the sister group of living jawed vertebrates (gnathostomes) and hence an important group for understanding the origin and diversity of vertebrates. In vertebrates and other metazoans, Hox genes determine cell fate along the anteroposterior axis of embryos and are implicated in driving morphological diversity. Invertebrates contain a single Hox cluster (either intact or fragmented), whereas elephant shark, coelacanth, and tetrapods contain four Hox clusters owing to two rounds of whole-genome duplication ("1R" and "2R") during early vertebrate evolution. By contrast, most teleost fishes contain up to eight Hox clusters because of an additional "teleost-specific" genome duplication event. By sequencing bacterial artificial chromosome (BAC) clones and the whole genome, here we provide evidence for at least six Hox clusters in the Japanese lamprey (Lethenteron japonicum). This suggests that the lamprey lineage has experienced an additional genome duplication after 1R and 2R. The relative age of lamprey and human paralogs supports this hypothesis. Compared with gnathostome Hox clusters, lamprey Hox clusters are unusually large. Several conserved noncoding elements (CNEs) were predicted in the Hox clusters of lamprey, elephant shark, and human. Transgenic zebrafish assay indicated the potential of CNEs to function as enhancers. Interestingly, CNEs in individual lamprey Hox clusters are frequently conserved in multiple Hox clusters in elephant shark and human, implying a many-to-many orthology relationship between lamprey and gnathostome Hox clusters. Such a relationship suggests that the first two rounds of genome duplication may have occurred independently in the lamprey and gnathostome lineages.
ESTHER : Mehta_2013_Proc.Natl.Acad.Sci.U.S.A_110_16044
PubMedSearch : Mehta_2013_Proc.Natl.Acad.Sci.U.S.A_110_16044
PubMedID: 24043829
Gene_locus related to this paper: lamja-ACHE