Hirano T

References (13)

Title : A Randomized, Placebo-controlled Study to Evaluate the Efficacy and Safety of Adding Omarigliptin to Insulin Therapy in Japanese Patients with Type 2 Diabetes and Inadequate Glycemic Control - Kadowaki_2021_Diabetes.Obes.Metab__
Author(s) : Kadowaki T , Seino Y , Kaku K , Okamoto T , Kameya M , Sato A , Hirano T , Oshima N , Gantz I , O'Neill EA , Engel SS
Ref : Diabetes Obes Metab , : , 2021
Abstract : AIMS: To evaluate the efficacy and safety of adding the once-weekly oral DPP-4 inhibitor omarigliptin to treatment of Japanese patients with type 2 diabetes and inadequate glycemic control on insulin monotherapy. MATERIALS AND METHODS: In a 52-week clinical trial, Japanese patients on insulin monotherapy were randomized to once-weekly omarigliptin 25 mg (N=123) or placebo (N=61) for a 16-week, double-blind, placebo-controlled period. After Week 16, patients continued or switched to omarigliptin for a 36-week open-label period. RESULTS: From a mean baseline of approximately 8.8%, the Week 16 least squares mean changes in HbA1c were -0.61% (omarigliptin) and 0.29% (placebo); the between-group difference was -0.90%, p<0.001. At Week 52, the mean change from baseline in HbA1c was -0.57% in both the group on omarigliptin for 52 weeks and the group on omarigliptin for 36 weeks (switched from placebo at Week 16). During the first 16 weeks of treatment, the incidences of adverse events (AEs), serious AEs, drug-related AEs, and discontinuation from trial medication due to an AE were similar in both groups. A slight increase in incidence of symptomatic hypoglycaemia was observed in the omarigliptin group (n=13 [10.6%]) compared with placebo (n=4 [6.6%]). No severe hypoglycaemia was reported during the study. No new safety signals emerged with treatment beyond Week 16 through Week 52. CONCLUSION: The addition of once-weekly omarigliptin to insulin therapy for up to 52 weeks was generally well tolerated and provided clinically meaningful improvement in glycemic control throughout the trial period. (ClinicalTrials.gov: NCT02906709). This article is protected by copyright. All rights reserved.
ESTHER : Kadowaki_2021_Diabetes.Obes.Metab__
PubMedSearch : Kadowaki_2021_Diabetes.Obes.Metab__
PubMedID: 33512755

Title : NOAEL-dose of a neonicotinoid pesticide, clothianidin, acutely induce anxiety-related behavior with human-audible vocalizations in male mice in a novel environment - Hirano_2018_Toxicol.Lett_282_57
Author(s) : Hirano T , Yanai S , Takada T , Yoneda N , Omotehara T , Kubota N , Minami K , Yamamoto A , Mantani Y , Yokoyama T , Kitagawa H , Hoshi N
Ref : Toxicol Lett , 282 :57 , 2018
Abstract : Neonicotinoids are novel systemic pesticides acting as agonists on the nicotinic acetylcholine receptors (nAChRs) of insects. Experimental studies have revealed that neonicotinoids pose potential risks for the nervous systems of non-target species, but the brain regions responsible for their behavioral effects remain incompletely understood. This study aimed to assess the neurobehavioral effects of clothianidin (CTD), a later neonicotinoid developed in 2001 and widely used worldwide, and to explore the target regions of neonicotinoids in the mammalian brain. A single-administration of 5 or 50mg/kg CTD to male C57BL/6N mice at or below the no-observed-adverse-effect level (NOAEL) induced an acute increase in anxiety during the elevated plus-maze test. In addition, mice in the CTD-administered group spontaneously emitted human-audible vocalizations (4-16kHz), which are behavioral signs of aversive emotions, and showed increased numbers of c-fos immunoreactive cells in the paraventricular thalamic nucleus and dentate gyrus of the hippocampus. In conclusion, mice exposed to NOAEL-dose CTD would be rendered vulnerable to a novel environment via the activation of thalamic and hippocampal regions related to stress responses. These findings should provide critical insight into the neurobehavioral effects of neonicotinoids on mammals.
ESTHER : Hirano_2018_Toxicol.Lett_282_57
PubMedSearch : Hirano_2018_Toxicol.Lett_282_57
PubMedID: 29030271

Title : Treatment of Myasthenia Gravis With High-Dose Cholinesterase Inhibitors and Calcineurin Inhibitors Caused Spontaneous Muscle Cramps in Patients - Masuda_2018_Clin.Neuropharmacol_41_164
Author(s) : Masuda M , Utsumi H , Tanaka S , Maeno A , Yamamoto M , Sugiyama K , Hirano T , Aizawa H
Ref : Clinical Neuropharmacology , 41 :164 , 2018
Abstract : OBJECTIVES: The objective of this study was to investigate the influence of treatment with cholinesterase inhibitors (ChEIs) and calcineurin inhibitors (CNIs) on the occurrence of cramps in myasthenia gravis (MG) patients. METHODS: The frequency and duration of cramp and serum electrolytes were evaluated in 81 patients with MG. The patients were classified using Myasthenia Gravis Foundation of America postintervention status scores based on the treatment and the responsiveness to the treatment. Quantitative MG score, MG activities of daily living score, MG composite score, or MG quality of life 15 score was used to assess the health-related quality of life (QOL). RESULTS: Muscle cramps developed in 44 (54.3%) of 81 MG patients. The scores of MG activities of daily living, MG composite, or MG-QOL 15-item questionnaire in patients with cramp were significantly higher than those in patients without cramps (P = 0.002, P = 0.01, or P = 0.0022, respectively). The serum magnesium concentrations were lower in patients treated with CNI (n = 16) than in those not treated with CNI (n = 65) (P = 0.002). The probability of cramps was significantly higher in patients treated with ChEIs (>/=180 mg/d) in addition to CNI than in patients who were treated with a low dose of ChEIs (<=60 mg/d) without concomitant CNI treatment (P = 0.017). CONCLUSIONS: Our data suggested that treatment with a high dose of ChEI and CNI accelerated the probability of cramps and reduced the QOL in MG patients.
ESTHER : Masuda_2018_Clin.Neuropharmacol_41_164
PubMedSearch : Masuda_2018_Clin.Neuropharmacol_41_164
PubMedID: 30130259

Title : Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface - Funahashi_2018_Front.Cell.Neurosci_12_140
Author(s) : Funahashi J , Tanaka H , Hirano T
Ref : Front Cell Neurosci , 12 :140 , 2018
Abstract : Fast repetitive synaptic transmission depends on efficient exocytosis and retrieval of synaptic vesicles around a presynaptic active zone. However, the functional organization of an active zone and regulatory mechanisms of exocytosis, endocytosis and reconstruction of release-competent synaptic vesicles have not been fully elucidated. By developing a novel visualization method, we attempted to identify the location of exocytosis of a single synaptic vesicle within an active zone and examined movement of synaptic vesicle protein synaptophysin (Syp) after exocytosis. Using cultured hippocampal neurons, we induced formation of active-zone-like membranes (AZLMs) directly adjacent and parallel to a glass surface coated with neuroligin, and imaged Syp fused to super-ecliptic pHluorin (Syp-SEP) after its translocation to the plasma membrane from a synaptic vesicle using total internal reflection fluorescence microscopy (TIRFM). An AZLM showed characteristic molecular and functional properties of a presynaptic active zone. It contained active zone proteins, cytomatrix at the active zone-associated structural protein (CAST), Bassoon, Piccolo, Munc13 and RIM, and showed an increase in intracellular Ca(2+) concentration upon electrical stimulation. In addition, single-pulse stimulation sometimes induced a transient increase of Syp-SEP signal followed by lateral spread in an AZLM, which was considered to reflect an exocytosis event of a single synaptic vesicle. The diffusion coefficient of Syp-SEP on the presynaptic plasma membrane after the membrane fusion was estimated to be 0.17-0.19 mum(2)/s, suggesting that Syp-SEP diffused without significant obstruction. Synchronous exocytosis just after the electrical stimulation tended to occur at multiple restricted sites within an AZLM, whereas locations of asynchronous release occurring later after the stimulation tended to be more scattered.
ESTHER : Funahashi_2018_Front.Cell.Neurosci_12_140
PubMedSearch : Funahashi_2018_Front.Cell.Neurosci_12_140
PubMedID: 29875634

Title : Anagliptin, a dipeptidyl peptidase-4 inhibitor, decreases macrophage infiltration and suppresses atherosclerosis in aortic and coronary arteries in cholesterol-fed rabbits - Hirano_2016_Metabolism_65_893
Author(s) : Hirano T , Yamashita S , Takahashi M , Hashimoto H , Mori Y , Goto M
Ref : Metabolism , 65 :893 , 2016
Abstract : INTRODUCTION: Several studies have demonstrated suppression of aortic atherosclerosis by dipeptidyl peptidase-4 (DPP-4) inhibitors in hypercholesterolemic mice. However, it remains unknown whether DPP-4 inhibitors also exert anti-atherogenic effects in coronary arteries. We examined the effect of anagliptin, a DPP-4 inhibitor, on atherosclerosis development in the aorta and coronary arteries in a high-cholesterol diet-fed rabbits. METHODS: Japanese white rabbits were fed either normal chow (n=8) or a diet containing 0.5% cholesterol (n=34) for 14weeks. Cholesterol-fed rabbits were given 0.3% anagliptin or not in drinking water (each n=16 and 18) for 12weeks. RESULTS: Dietary cholesterol intake markedly increased serum total cholesterol (TC) levels (1464+/-150mg/dL, mean+/-SE), and the most striking increase was observed among the major lipoproteins in very low-density lipoprotein (VLDL) as determined by high-performance liquid chromatography. No significant changes were observed in body weight, water intake, hemoglobin A1c, or glucose response to intravenous glucose loading following anagliptin administration. Anagliptin decreased TC and VLDL-cholesterol as well as cholesterol absorption markers sitosterol and campesterol slightly, although not significantly. Serum DPP-4 activity was suppressed by 82%, and active glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide levels were increased 2- to 3-fold by anagliptin treatment. Severe hypercholesterolemia resulted in the development of atherosclerosis in the aorta, and the ratio of atherosclerotic lesions to the total aortic surface area was 22+/-2%. Anagliptin suppressed the lesion ratio to 9+/-2% (p<0.001). Atherosclerotic lesions were clearly observed in the coronary arteries, where the mean intima-media area was enlarged, and intimal formation was developed. Anagliptin treatment attenuated the intima-media area and the intimal area by 43%. Alpha-smooth muscle actin-positive and macrophage-positive areas in the coronary arteries were suppressed by 66 and 75%, respectively, after anagliptin treatment. The aortic lesion ratio and the coronary intima area were correlated with each other (r=0.506, p<0.01), and each lesion correlated with TC in the whole cholesterol-fed rabbits. Gene expression of the proinflammatory cytokines tumor necrosis factor-alpha and interleukin-6 in the carotid arteries was markedly reduced by approximately 90%, and vascular DPP-4 activity was reduced by 66% after anagliptin treatment. CONCLUSIONS: We demonstrated for the first time that a DPP-4 inhibitor can substantially suppress plaque formation in coronary arteries with a marked reduction in macrophage accumulation likely via its anti-inflammatory properties.
ESTHER : Hirano_2016_Metabolism_65_893
PubMedSearch : Hirano_2016_Metabolism_65_893
PubMedID: 27173468

Title : The power of single molecule real-time sequencing technology in the de novo assembly of a eukaryotic genome - Sakai_2015_Sci.Rep_5_16780
Author(s) : Sakai H , Naito K , Ogiso-Tanaka E , Takahashi Y , Iseki K , Muto C , Satou K , Teruya K , Shiroma A , Shimoji M , Hirano T , Itoh T , Kaga A , Tomooka N
Ref : Sci Rep , 5 :16780 , 2015
Abstract : Second-generation sequencers (SGS) have been game-changing, achieving cost-effective whole genome sequencing in many non-model organisms. However, a large portion of the genomes still remains unassembled. We reconstructed azuki bean (Vigna angularis) genome using single molecule real-time (SMRT) sequencing technology and achieved the best contiguity and coverage among currently assembled legume crops. The SMRT-based assembly produced 100 times longer contigs with 100 times smaller amount of gaps compared to the SGS-based assemblies. A detailed comparison between the assemblies revealed that the SMRT-based assembly enabled a more comprehensive gene annotation than the SGS-based assemblies where thousands of genes were missing or fragmented. A chromosome-scale assembly was generated based on the high-density genetic map, covering 86% of the azuki bean genome. We demonstrated that SMRT technology, though still needed support of SGS data, achieved a near-complete assembly of a eukaryotic genome.
ESTHER : Sakai_2015_Sci.Rep_5_16780
PubMedSearch : Sakai_2015_Sci.Rep_5_16780
PubMedID: 26616024
Gene_locus related to this paper: phaan-a0a0s3rce3 , phaan-a0a0s3tc53 , vigrr-a0a1s3v914 , phaan-a0a0s3s998 , phaan-a0a0s3siv8 , phaan-a0a0l9uys5 , phaan-a0a0s3rp07 , phaan-a0a0s3rbq0 , phaan-a0a0s3smk7 , phaan-a0a0s3slm9 , phaan-a0a0l9uc60 , phaan-a0a0l9ucr8

Title : Multicolor Imaging of Endoplasmic Reticulum-Located Esterase As a Prodrug Activation Enzyme - Hakamata_2014_ACS.Med.Chem.Lett_5_321
Author(s) : Hakamata W , Tamura S , Hirano T , Nishio T
Ref : ACS Med Chem Lett , 5 :321 , 2014
Abstract : The carboxylesterase families of enzymes are key participants in phase I drug metabolism processes. Carboxylesterase families 1 and 2 are of particular clinical relevance. These enzymes produce endoplasmic reticulum localization signals, are primarily localized in the endoplasmic reticulum, and hydrolyze a wide range of ester-containing prodrugs into an activated form. In order to detect enzymes belonging to both families, we developed an optical multicolor imaging technique, which provides a distinct color window for multicolor imaging. This technique required the design and synthesis of three new mechanistic colored probes that fluoresce red, green, or blue and are based on the quinone methide cleavage process. These activity-based probes allow rapid and clear visualization with high specificity against the endoplasmic reticulum in cultured cells based on endoplasmic reticulum localized esterases including both families of carboxylesterase enzymes.
ESTHER : Hakamata_2014_ACS.Med.Chem.Lett_5_321
PubMedSearch : Hakamata_2014_ACS.Med.Chem.Lett_5_321
PubMedID: 24900834

Title : Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology - Satou_2014_Genome.Announc_2_e00286
Author(s) : Satou K , Shiroma A , Teruya K , Shimoji M , Nakano K , Juan A , Tamotsu H , Terabayashi Y , Aoyama M , Teruya M , Suzuki R , Matsuda M , Sekine A , Kinjo N , Kinjo F , Yamaoka Y , Hirano T
Ref : Genome Announc , 2 : , 2014
Abstract : We report the complete genome sequences of eight Helicobacter pylori strains isolated from patients with gastrointestinal diseases in Okinawa, Japan. Whole-genome sequencing and DNA methylation detection were performed using the PacBio platform. De novo assembly determined a single, complete contig for each strain. Furthermore, methylation analysis identified virulence factor genotype-dependent motifs.
ESTHER : Satou_2014_Genome.Announc_2_e00286
PubMedSearch : Satou_2014_Genome.Announc_2_e00286
PubMedID: 24744331

Title : Construction of a new recombinant protein expression system in the basidiomycetous yeast Cryptococcus sp. strain S-2 and enhancement of the production of a cutinase-like enzyme - Masaki_2012_Appl.Microbiol.Biotechnol_93_1627
Author(s) : Masaki K , Tsuchioka H , Hirano T , Kato M , Ikeda H , Iefuji H
Ref : Applied Microbiology & Biotechnology , 93 :1627 , 2012
Abstract : Yeast host-vector systems have been very successful in expressing recombinant proteins. However, because there are some proteins that cannot be expressed with existing systems, there is a need for new yeast expression systems. Here we describe a new host-vector system based on the basidiomycetous yeast Cryptococcus sp. strain S-2 (S-2). Two advantages of S-2 are that it naturally produces some very useful enzymes, so it would be a good system for expressing multiple copies of some of its genes, and that, it is a nonhazardous species. The orotate phosphoribosyltransferase (OPRTase, EC 2.4.2.10) gene (URA5) was selected as a selectable marker for transformation in the new host-vector system. URA5 was isolated and introduced into a uracil auxotroph of S-2 by electroporation. To demonstrate the S-2 system, we selected one of its unique enzymes, a plastic-degrading cutinase-like enzyme (CLE). We were able to insert multiple copies of the CLE gene (CLE1) into the chromosomes in a high fraction of the targeted cells. Under optimal conditions, one transformant exhibited 3.5 times higher CLE activity than the wild type. Expression vectors, including an inducible promoter (the promoter for the xylanase or alpha-amylase gene), were constructed for recombinant protein production, and green fluorescent protein was expressed under the control of these promoters. The xylanase promoter was more tightly controlled. Furthermore, putting CLE1 under the control of the xylanase promoter, which is induced by xylose, increased CLE activity of the culture medium to approximately 15 times greater than that of the wild type.
ESTHER : Masaki_2012_Appl.Microbiol.Biotechnol_93_1627
PubMedSearch : Masaki_2012_Appl.Microbiol.Biotechnol_93_1627
PubMedID: 22083278

Title : Postsynaptic glutamate receptor delta family contributes to presynaptic terminal differentiation and establishment of synaptic transmission - Kuroyanagi_2009_Proc.Natl.Acad.Sci.U.S.A_106_4912
Author(s) : Kuroyanagi T , Yokoyama M , Hirano T
Ref : Proc Natl Acad Sci U S A , 106 :4912 , 2009
Abstract : Synaptic adhesion molecules such as neuroligin are involved in synapse formation, whereas ionotropic transmitter receptors mediate fast synaptic transmission. In mutant mice deficient in the glutamate receptor delta2 subunit (delta2), the number of synapses between granule neurons (GNs) and a Purkinje neuron (PN) in the cerebellum is reduced. Here, we have examined the role of delta2 in synapse formation using culture preparations. First, we found that the size and number of GN presynaptic terminals on a PN in the primary culture prepared from knockout mice were smaller than those in control culture. Next we expressed delta2 in nonneuronal human embryonic kidney (HEK) cells and cocultured them with GNs. Punctate structures expressing marker proteins for glutamatergic presynaptic terminals were accumulated around the HEK cells. Furthermore, HEK cells expressing both delta2 and GluR1, a glutamate receptor subunit forming a functional glutamate-gated ion channel, showed postsynaptic current. Deletion of the extracellular leucine/isoleucine/valine binding protein (LIVBP) domain of delta2 abolished the induction ability, and the LIVBP domain directly fused to a transmembrane sequence was sufficient to induce presynaptic differentiation. Furthermore, a mutant GluR1 whose LIVBP domain was replaced with the delta2 LIVBP domain was sufficient by itself to establish synaptic transmission. Another member of delta glutamate receptor family delta1 also induced presynaptic differentiation. Thus, the delta glutamate receptor subfamily can induce the differentiation of glutamatergic presynaptic terminals and contribute to the establishment of synaptic transmission.
ESTHER : Kuroyanagi_2009_Proc.Natl.Acad.Sci.U.S.A_106_4912
PubMedSearch : Kuroyanagi_2009_Proc.Natl.Acad.Sci.U.S.A_106_4912
PubMedID: 19258455

Title : Thioesterase activity and subcellular localization of acylprotein thioesterase 1\/lysophospholipase 1 - Hirano_2009_Biochim.Biophys.Acta_1791_797
Author(s) : Hirano T , Kishi M , Sugimoto H , Taguchi R , Obinata H , Ohshima N , Tatei K , Izumi T
Ref : Biochimica & Biophysica Acta , 1791 :797 , 2009
Abstract : Acylprotein thioesterase 1 (APT1), also known as lysophospholipase 1, is an important enzyme responsible for depalmitoylation of palmitoyl proteins. To clarify the substrate selectivity and the intracellular function of APT1, we performed kinetic analyses and competition assays using a recombinant human APT1 (hAPT1) and investigated the subcellular localization. For this purpose, an assay for thioesterase activity against a synthetic palmitoyl peptide using liquid chromatography/mass spectrometry was established. The thioesterase activity of hAPT1 was most active at neutral pH, and did not require Ca(2+) for its maximum activity. The K(M) values for thioesterase and lysophospholipase (against lysophosphatidylcholine) activities were 3.49 and 27.3 microM, and the V(max) values were 27.3 and 1.62 micromol/min/mg, respectively. Thus, hAPT1 revealed much higher thioesterase activity than lysophospholipase activity. One activity was competitively inhibited by another substrate in the presence of both substrates. Immunocytochemical and Western blot analyses revealed that endogenous and overexpressed hAPT1 were mainly localized in the cytosol, while some signals were detected in the plasma membrane, the nuclear membrane and ER in HEK293 cells. These results suggest that eliminating palmitoylated proteins and lysophospholipids from cytosol is one of the functions of hAPT1.
ESTHER : Hirano_2009_Biochim.Biophys.Acta_1791_797
PubMedSearch : Hirano_2009_Biochim.Biophys.Acta_1791_797
PubMedID: 19439193

Title : Characterization of secretory intestinal transport of the lactone form of CPT-11 - Takemoto_2006_Cancer.Chemother.Pharmacol_57_129
Author(s) : Takemoto I , Itagaki S , Chiba M , Itoh T , Hirano T , Iseki K
Ref : Cancer Chemother Pharmacol , 57 :129 , 2006
Abstract : PURPOSE: It has been reported that a significant portion of the lactone form of 7-ethyl-10-(4-[1-piperidino]-1-piperidino)-carbonyloxy-camptothecin (CPT-11) is excreted into the gastrointestinal lumen via the intestinal membrane and that carboxylesterase activity, which converts CPT-11 to SN-38, was detected in the intestine. It is possible that a reduction in the excretion of CPT-11 lactone into the gastrointestinal lumen induces the gastrointestinal toxicity. The purpose of this study was to investigate the characteristics of transporter(s) that contribute to the jejunal efflux of the lactone form of CPT-11.
METHODS: The serosal-to-mucosal permeation rate of CPT-11 lactone was investigated in everted sac studies.
RESULTS: The secretory transport required metabolic energy and was diminished by sulfobromophthalein (BSP) and 1-naphthol, inhibitors of the ME3277 transport system. However, inhibitors of breast cancer resistance protein (Bcrp), multidrug resistance-associated protein 2 (Mrp2) and P-glycoprotein (P-gp) did not affect the secretion of CPT-11 lactone.
CONCLUSIONS: The results suggest that a specific transport system, which is identical to the ME3277 transport system, plays a major role in the secretion of CPT-11 lactone.
ESTHER : Takemoto_2006_Cancer.Chemother.Pharmacol_57_129
PubMedSearch : Takemoto_2006_Cancer.Chemother.Pharmacol_57_129
PubMedID: 16003561

Title : Measurement of the serum lipoprotein lipase concentration is useful for studying triglyceride metabolism: Comparison with postheparin plasma - Hirano_2004_Metabolism_53_526
Author(s) : Hirano T , Nishioka F , Murakami T
Ref : Metabolism , 53 :526 , 2004
Abstract : The catalytically inactive form of lipoprotein lipase (LPL) is detectable at high levels in serum, although its physiologic role remains unknown. The aim of this study was to elucidate the clinical significance of serum LPL compared with postheparin LPL or the net increment (Delta) of LPL (postheparin - preheparin LPL). We measured the LPL mass before and 15 minutes after the injection of heparin in 164 subjects with hyperlipidemia. LPL mass was measured by a sensitive sandwich enzyme-linked immunosorbent assay (ELISA). Serum LPL was one fifth of the postheparin LPL concentration. There was a weak correlation between the serum LPL and postheparin LPL concentrations (r =.225, P </=.005). The Delta LPL concentration was strongly related to the postheparin LPL concentration (r =.965, P </=.0001), but not to the preheparin LPL mass, suggesting that the weak correlation between serum LPL and postheparin LPL levels was attributable to contamination of postheparin plasma by pre-existing LPL (preheparin LPL). Both serum and postheparin LPL were significantly lower in diabetic patients and in subjects with high levels of triglyceride or low levels of high-density lipoprotein (HDL). Serum LPL was correlated negatively with triglyceride, remnants, and insulin resistance and was positively correlated with HDL cholesterol and low-density lipoprotein (LDL) size. Postheparin LPL was strongly correlated with HDL cholesterol, but not with other parameters, as was serum LPL. Delta LPL mass did not show a closer association with triglyceride metabolism than postheparin LPL or preheparin LPL. In conclusion, serum LPL measurement is simple and seems to be useful for studying triglyceride metabolism.
ESTHER : Hirano_2004_Metabolism_53_526
PubMedSearch : Hirano_2004_Metabolism_53_526
PubMedID: 15045703