References (2)

Title : Kuwanon G protects HT22 cells from advanced glycation end product-induced damage - Gan_2021_Exp.Ther.Med_21_425
Author(s) : Gan WJ , Gao CL , Zhang WQ , Gu JL , Zhao TT , Guo HL , Zhou H , Xu Y , Yu LL , Li LF , Gui DK , Xu YH
Ref : Exp Ther Med , 21 :425 , 2021
Abstract : The incidence of diabetic encephalopathy is increasing as the population ages. Evidence suggests that formation and accumulation of advanced glycation end products (AGEs) plays a pivotal role in disease progression, but limited research has been carried out in this area. A previous study demonstrated that Kuwanon G (KWG) had significant anti-oxidative stress and anti-inflammatory properties. As AGEs are oxidative products and inflammation is involved in their generation it is hypothesized that KWG may have effects against AGE-induced neuronal damage. In the present study, mouse hippocampal neuronal cell line HT22 was used. KWG was shown to significantly inhibit AGE-induced cell apoptosis in comparison with a control treatment, as determined by both MTT and flow cytometry. Compared with the AGEs group, expression of pro-apoptotic protein Bax was reduced and expression of anti-apoptotic protein Bcl-2 was increased in the AGEs + KWG group. Both intracellular and extracellular levels of acetylcholine and choline acetyltransferase were significantly elevated after KWG administration in comparison with controls whilethe level of acetylcholinesterase decreased. These changes in protein expression were accompanied by increased levels of superoxide dismutase and glutathione peroxidase synthesis and reduced production of malondialdehyde and reactive oxygen species. Intracellular signaling pathway protein levels were determined by western blot and immunocytochemistry. KWG administration was found to prevent AGE-induced changes to the phosphorylation levels of Akt, IkappaB-alpha, glycogen synthase kinase 3 (GSK3)-alpha and beta, p38 MAPK and NF-kappaB p65 suggesting a potential neuroprotective effect of KWG against AGE-induced damage was via the PI3K/Akt/GSK3alphabeta signaling pathway. The findings of the present study suggest that KWG may be a potential treatment for diabetic encephalopathy.
ESTHER : Gan_2021_Exp.Ther.Med_21_425
PubMedSearch : Gan_2021_Exp.Ther.Med_21_425
PubMedID: 33747164

Title : Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans - Lang_2010_BMB.Rep_43_573
Author(s) : Lang GJ , Zhang MY , Li BL , Yu LL , Lu XM , Zhang CX
Ref : BMB Rep , 43 :573 , 2010
Abstract : Several organophosphorus (OP) insecticides can selectively kill the silkworm maggot, Exorista sorbillans (Es) (Diptera: Tachinidae), while not obviously affecting the host (Bombyx mori) larvae, but the mechanism is not yet clear. In this study, the cDNA encoding an acetylcholinesterase (AChE) from the field Es was isolated. One point mutation (Gly353Ala) was identified. The Es-353G AChE and Es-353A AChE were expressed in baculovirus- insect cell system, respectively. The inhibition results showed that for eserine and Chlorpyrifos, Es-353A AChE was significantly less sensitive than Es-353G AChE. Meanwhile, comparison of the I(50) values of eserine, dichlorvos, Chlorpyrifos and omethoate of recombinant Es AChEs with its host (Bombyx mori) AChEs indicated that, both Es AChEs are more sensitive than B. mori AChEs. The results give an insight of the mechanism that some OP insecticides can selectively kills Es while without distinct effect on its host, B. mori.
ESTHER : Lang_2010_BMB.Rep_43_573
PubMedSearch : Lang_2010_BMB.Rep_43_573
PubMedID: 20797321
Gene_locus related to this paper: exoso-ACHE2